We narrowed to 9,184 results for: Pol;

1. pDONR223-RPS6KC1

   Plasmid

   #23460

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   RPS6KC1 (RPS6KC1 Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

2. pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q139

   Plasmid

   #111738

   Purpose

   Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

   Depositor

   Cheryl Arrowsmith

   Article

   Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

   Insert

   HTT (HTT Human)
   

   Use

   Baculovirus expression

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Q139 (polyQ repeat)

   Promoter

   CMV and P10

   Available Since

   June 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDONR223-PLK1

   Plasmid

   #23867

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   PLK1 (PLK1 Human)
   

   Use

   Gateway donor vector

   Mutation

   A3P

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pDONR223-PIK4CA

   Plasmid

   #23541

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   PIK4CA (PI4KA Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pAAV-hSyn-Htt171-18Q-myc-WPRE

   Plasmid

   #107936

   Purpose

   Adeno-Associated viral (AAV) vector plasmid expressing exon1 of wild type HTT (wtHTT, 18 polyQ repeats) in neurons

   Depositor

   Johan Jakobsson

   Article

   Pircs et al Cell Rep. 2018 Aug 7;24(6):1397-1406. do

   Insert

   Exon 1 of wild type HTT (HTT Human)
   

   Use

   AAV

   Promoter

   hSynapsin

   Available Since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q48

   Plasmid

   #111747

   Purpose

   Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

   Depositor

   Cheryl Arrowsmith

   Article

   Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

   Insert

   HTT (HTT Human)
   

   Use

   Baculovirus expression

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Q48 (polyQ repeat)

   Promoter

   CMV and P10

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q51

   Plasmid

   #111734

   Purpose

   Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

   Depositor

   Cheryl Arrowsmith

   Article

   Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

   Insert

   HTT (HTT Human)
   

   Use

   Baculovirus expression

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Q51 (polyQ repeat)

   Promoter

   CMV and P10

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-TEV-2xSTII-PSMB5-PSMB6-PSMB7

   Plasmid

   #214140

   Purpose

   Insect cell expression of human 20S CP beta-subunits with a c-terminal twin-strep-tag on beta7

   Depositor

   Brenda Schulman

   Article

   Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

   Inserts

   PSMB1 (PSMB1 Human)
   PSMB2 (PSMB2 Human)
   PSMB3 (PSMB3 Human)
   PSMB4 (PSMB4 Human)
   PSMB5 (PSMB5 Human)
   PSMB6 (PSMB6 Human)
   PSMB7 (PSMB7 Human)
   

   Tags

   2xSTII

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q66

   Plasmid

   #111750

   Purpose

   Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

   Depositor

   Cheryl Arrowsmith

   Article

   Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

   Insert

   HTT (HTT Human)
   

   Use

   Baculovirus expression

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Q66 (polyQ repeat)

   Promoter

   CMV and P10

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMXs-GNL

    Plasmid

    #188232

    Purpose

    Polycistronic human Glis1, LIN28 and NANOG were cloned into the pMXs vector

    Depositor

    Young Tang

    Article

    Wang et al Biol Open. 2019 Dec 5;8(12). pii: 8/12/b

    Insert

    Glis1, NANOG and LIN28A separated by 2A sequence (GLIS1, NANOG, LIN28A Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q73

    Plasmid

    #111724

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q73 (polyQ repeat)

    Promoter

    CMV and P10

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q73

    Plasmid

    #111728

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q73 (polyQ repeat)

    Promoter

    CMV and P10

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSBbi-moxGFP-P2A-T2A-Puro

    Plasmid

    #176893

    Purpose

    Sleeping Beauty transposon-based vector for mammalian polycistronic expression of moxGFP and two additional genes of interest

    Depositor

    Jorge Luis Arias-Arias

    Article

    Acon et al iScience. 2021 Nov 8;24(12):103407. doi:

    Insert

    moxGFP-P2A-T2A
    

    Use

    Transposon

    Tags

    moxGFP

    Expression

    Mammalian

    Promoter

    EF1a/RPBSA

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-PSMB5-PSMB6-PSMB7-TEV-2xSTII

    Plasmid

    #214139

    Purpose

    Insect cell expression of human 20S CP beta-subunits with a c-terminal twin-strep-tag on beta2

    Depositor

    Brenda Schulman

    Article

    Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

    Inserts

    PSMB1 (PSMB1 Human)
    PSMB2 (PSMB2 Human)
    PSMB3 (PSMB3 Human)
    PSMB4 (PSMB4 Human)
    PSMB5 (PSMB5 Human)
    PSMB6 (PSMB6 Human)
    PSMB7 (PSMB7 Human)
    

    Tags

    2xSTII

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLEX307 hDVL1

    Plasmid

    #102863

    Purpose

    Expresses human DVL1 in mammalian cells

    Depositor

    Henry Ho

    Article

    Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

    Insert

    human Dishevelled 1 (DVL1) (DVL1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Changed alanine 2 to glycine. Likely due to poly…

    Promoter

    EF1A

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. IMPT-6213

    Plasmid

    #99333

    Purpose

    AT2R N terminal BRIL, linker GSGS, delta aa 1-34, delta aa 336-363

    Depositor

    Raymond Stevens

    Article

    Zhang et al Nature. 2017 Apr 20;544(7650):327-332. d

    Insert

    AGTR2 (AGTR2 Human)
    

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q79

    Plasmid

    #111725

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q79 (polyQ repeat)

    Promoter

    CMV and P10

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-Lyso-INPP4B-mCherry

    Plasmid

    #184055

    Purpose

    Lysosome targeted inositol polyphosphate-4-phosphatase type II B; tagged with mCherry.

    Depositor

    Jin Zhang

    Article

    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

    Insert

    LGP120 Lyso-INPP4B-mCherry (LAMP1, INPP4B Human)
    

    Tags

    Lysosome-associated membrane protein 1 (LAMP1). a…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDONR223-RPS6KA4

    Plasmid

    #23641

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    RPS6KA4 (RPS6KA4 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pFastbac-Flag-BRCA1

    Plasmid

    #223228

    Purpose

    Insect expression construct for Flag-tagged BRCA1 protein production and purification

    Depositor

    Weixing Zhao

    Article

    Wang et al Mol Cell. 2023 Oct 19;83(20):3679-3691.e

    Insert

    Breast Cancer 1 (BRCA1 Human)
    

    Tags

    Flag

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCS2-Prom1-TEVp-TwinStrep-His / IRES2 / Pcdh21-TEVp-Myc-Flag

    Plasmid

    #210820

    Purpose

    Mammalian overexpression vector for polycistronic co-expression of C-terminally Strep and His tagged human Prom1s1 (WT) and C-terminally Myc and Flag tagged human Pchd21 (WT)

    Depositor

    Luke Chao

    Article

    Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

    Inserts

    Prominin 1 (PROM1 Human)
    Protocadherin 21 (CDHR1 Human)
    

    Tags

    Myc, 3xFlag and TwinStrep, 10xHis

    Expression

    Mammalian

    Promoter

    CMV and CMV / IRES2

    Available Since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSBbi-moxGFP-P2A-T2A-cMyc-Puro

    Plasmid

    #176892

    Purpose

    Sleeping Beauty transposon-based vector for mammalian polycistronic expression of moxGFP, c-Myc and an additional gene of interest

    Depositor

    Jorge Luis Arias-Arias

    Article

    Acon et al iScience. 2021 Nov 8;24(12):103407. doi:

    Insert

    moxGFP-P2A-T2A-c-Myc
    

    Use

    Transposon

    Tags

    Myc and moxGFP

    Expression

    Mammalian

    Promoter

    EF1a/RPBSA

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLIB_3xFlag-His6-nsp13 (SARS-CoV-2)

    Plasmid

    #169189

    Purpose

    Baculoviral transfer vector to express 3xFlag-His6-nsp13 (SARS-CoV-2) in insect cells

    Depositor

    John Diffley

    Article

    Zeng et al Biochem J. 2021 Jul 16;478(13):2405-2423

    Insert

    3xFlag-His6-nsp13 (ORF1ab Synthetic, SARS-CoV-2)
    

    Tags

    3xFlag-His6

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLKO.puro_shGLS

    Plasmid

    #110335

    Purpose

    shGLS (Target CAACTGGCCAAATTCAGTC), silence human GLS gene, puromycin selection

    Depositor

    Sandra Martha Gomes Dias

    Article

    Dias et al Oncogene. 2020 Jan;39(3):690-702. doi: 1

    Insert

    GLS glutaminase (GLS Synthetic, Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6 (RNA PolIII)

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q52

    Plasmid

    #111748

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q52 (polyQ repeat)

    Promoter

    CMV and P10

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q24

    Plasmid

    #111742

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q24 (polyQ repeat)

    Promoter

    CMV and P10

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHAGE2-TetOminiCMV-SK

    Plasmid

    #136577

    Purpose

    Dox-inducible polycistronic lentiviral vector expressing mouse Sox2, Klf4

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Sox2-T2A-Klf4 (Klf4, Sox2 Mouse)
    

    Use

    Lentiviral

    Expression

    Bacterial

    Promoter

    tetO-miniCMV (dox-inducible)

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDONR223-RPS6KA6

    Plasmid

    #23540

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    RPS6KA6 (RPS6KA6 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

29. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q25

    Plasmid

    #111743

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q25 (polyQ repeat)

    Promoter

    CMV and P10

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBIG2ab_nsp14/nsp10-6His-3xFlag (SARS-CoV-2)

    Plasmid

    #169164

    Purpose

    Baculoviral transfer vector to co-express SARS-CoV-2 nsp14 and nsp10 in insect cells

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    nsp14/nsp10-6His-3xFlag (ORF1ab Synthetic, SARS-CoV-2)
    

    Tags

    6His-3xFlag (nsp10 C-terminus)

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge