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EF1A_Hygro_P2A_RUNX1_R118G_Barcode

Plasmid

#170970

Purpose

Overexpression of RUNX1 R118G mutant protein.

Depositor

Prashant Mali

Article

Ozturk et al Cell Rep. 2024 Jul 4;43(7):114436. doi:

Insert

RUNX1 (RUNX1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

R118G

Promoter

Ef1A

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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EF1A_Hygro_P2A_RUNX1_G100V_Barcode

Plasmid

#170955

Purpose

Overexpression of RUNX1 G100V mutant protein.

Depositor

Prashant Mali

Article

Ozturk et al Cell Rep. 2024 Jul 4;43(7):114436. doi:

Insert

RUNX1 (RUNX1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

G100V

Promoter

Ef1A

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-AIF

Plasmid

#16182

Depositor

Huda Zoghbi

Article

Lim et al Cell. 2006 May 19. 125(4):801-14.

Insert

Apoptosis-inducing factor (AIFM1 Human)

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only

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NP-11-63

Plasmid

#234358

Purpose

Expressing and purifying R.rubrum rubisco using a his-sumo tag

Depositor

David Savage

Article

Prywes et al Nature. 2025 Jan 22. doi: 10.1038/s41586

Insert

R.rubrum rubisco

Use

Synthetic Biology

Tags

N-term SUMO tag

Promoter

T7

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pB-MultiplexedReporter-MOR170-1

Plasmid

#129461

Purpose

piggyBac transposon vector encoding the receptor MOR170-1 and the barcoded CRE reporter gene

Depositor

Sriram Kosuri

Article

Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

Insert

MOR170-1 (Olfr895 Mouse)

Use

Synthetic Biology

Tags

LUCY tag, Rho tag

Expression

Mammalian

Promoter

TRE (Tet-On)

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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FUW-Gata2

Plasmid

#193087

Purpose

constitutive expression of mouse Gata2 in mammalian cells

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

Gata2 (Gata2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Ubc

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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LCV2 IRF3 KO

Plasmid

#217446

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human IRF3

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

sgRNA targeting IRF3 (IRF3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pPapi

Plasmid

#96921

Purpose

expression of S aureus SaCas9 (SauCas9) and two pol III promoters for an Sa gRNA and an Sp gRNA. Intended to be used in SpCas9-expressing cell lines for dual Cas9 "Big Papi" screens. aka pXPR207.

Depositor

John Doench, David Root

Article

Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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STK11 gRNA (BRDN0001146880)

Plasmid

#75912

Purpose

3rd generation lentiviral gRNA plasmid targeting human STK11

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

STK11 (STK11 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PKD1 gRNA (BRDN0001148399)

Plasmid

#76842

Purpose

3rd generation lentiviral gRNA plasmid targeting human PKD1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PKD1 (PKD1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-SFXN1

Plasmid

#110634

Purpose

Retroviral expression of Flag-SFXN1 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Science. 2018 Nov 16;362(6416). pii: 362

Insert

SFXN1 (SFXN1 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

codon-optimized cDNA (no amino acid mutations)

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pGLOW79

Plasmid

#177339

Purpose

C. elegans mNeonGreen co-injection marker

Depositor

Ryan Doonan

Article

Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

Insert

Pmyo-3::mNeonGreen

Tags

mNeonGreen

Expression

Worm

Promoter

myo-3

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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PKT2/CLP-AKT

Plasmid

#20281

Depositor

John Ohlfest

Article

Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

Insert

AKT (AKT1 Human)

Use

Transposon

Available Since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_4D5-Low_CAR-mCherry_pGK_4D5-Medium_SynNotch_RHL144

Plasmid

#164828

Purpose

Lentiviral vector for Gal4 inducible AntiHER2 4D5-Low CAR-mCherry and constitutive expression of antiHER2 4D5-Medium Gal4VP64 Synthetic Notch

Depositor

Wendell Lim

Article

Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

Inserts

AntiHER2 4D5-Low Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
AntiHER2 4D5-Medium Gal4VP64 synthetic Notch receptor

Use

Lentiviral

Tags

mCherry

Promoter

miniCMV and pGK

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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TVBB N-term-mNeongreen

Plasmid

#169226

Purpose

Targeting vector backbone to support a knock-in of mNeongreen-Linker at the N-terminus of a target locus

Depositor

Hugo Snippert

Article

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mNeongreen

Use

Targeting vector backbone

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pTET2-lucCP

Plasmid

#198353

Purpose

Part of Renilla reporter system, encodes Firefly luciferase

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

Firefly Luciferase

Use

Luciferase

Expression

Mammalian

Promoter

pTET2

Available Since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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EYFP-p65

Plasmid

#111192

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Schmid et al J Biol Chem. 2000 Jun 2;275(22):17035-42

Insert

RELA (RELA Human)

Tags

EYFP

Expression

Mammalian

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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NSUN2

Plasmid

#155793

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

NSUN2 (NSUN2 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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SPHK2 gRNA (BRDN0001148465)

Plasmid

#77093

Purpose

3rd generation lentiviral gRNA plasmid targeting human SPHK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

SPHK2 (SPHK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pHLmMBP-11

Plasmid

#72349

Purpose

Mammalian expression of secreted mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

Depositor

Luca Jovine

Article

Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

Type

Empty backbone

Tags

6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…

Expression

Mammalian

Promoter

CMV enhancer + chicken beta-actin promoter

Available Since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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