We narrowed to 13,291 results for: cDNA

1. pcDNA-ELYS-emerin-EGFP-polyA

   Plasmid

   #59745

   Purpose

   Express ELYS-emerin-EGFP fusion protein in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   ELYS-emerin-EGFP
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5 FRT TO Myc ArC Long WT

   Plasmid

   #59809

   Purpose

   Allows the integration of Myc ArC Long in the genome and Tet-inducible expression.

   Depositor

   Stephen Taylor

   Article

   Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

   Insert

   Aurora C (AURKC Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available Since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA 3.1 (+) FLAG-Ago2 S387E

   Plasmid

   #60653

   Purpose

   Expresses CMV-FLAG-Ago2 (N-term tag) S387E mutant in mammalian cells

   Depositor

   Carl Novina

   Article

   Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

   Insert

   Argonaute 2 (AGO2 Human)
   

   Use

   Tags

   CMV-FLAG-Ago2 (N-term tag)

   Expression

   Mammalian

   Mutation

   S387E mutant

   Promoter

   CMV

   Available Since

   Dec. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-CD16.7-hPC1-eGFP-3HA

   Plasmid

   #108401

   Purpose

   Express cytoplasmic tail human PC1 from amino acid 4077 to 4302 with a membrane anchor

   Depositor

   Gregory Germino

   Article

   Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

   Insert

   PKD1 (PKD1 Human)
   

   Use

   Tags

   eGFP-3HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1 FLIPglu-30uDelta13V ECFP-W67G

   Plasmid

   #18017

   Depositor

   Wolf Frommer

   Article

   Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

   Insert

   FLIPglu D154A Delta13V ECFP-W67G
   

   Use

   Tags

   ECFP-W67G and Venus

   Expression

   Mammalian

   Mutation

   mglB-D154A; non-functional ECFP

   Promoter

   Available Since

   Feb. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-bJun-C-mSOG-IRES-mCherry

   Plasmid

   #136600

   Purpose

   Encodes bJun domain tagged with the C-terminal fragment of split-miniSOG (mSOG-Ja95-140).

   Depositor

   John Ngo

   Article

   Boassa et al Cell Chem Biol. 2019 Oct 17;26(10):1407-

   Insert

   bJun-C-mSOG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-R536C-WLS-HA-IRES-GFP

   Plasmid

   #178065

   Purpose

   Expression of WLS-HA protein (R536C mutant)

   Depositor

   Joseph Gleeson

   Article

   Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

   Insert

   WLS (WLS Human)
   

   Use

   Tags

   HA and IRES-GFP

   Expression

   Mammalian

   Mutation

   R536C

   Promoter

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(-) Full length Her2(ERBB2)-Fc

   Plasmid

   #194330

   Purpose

   Mammalian expression of full length Human epidermal growth factor (Her)-2-Fc fusion protein

   Depositor

   Alexander McLellan

   Insert

   Her2-Fc (ERBB2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBABE-neo largeTcDNA delta434-444

   Plasmid

   #8582

   Depositor

   Bob Weinberg

   Article

   Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

   Insert

   large T antigen (SV40gp6 simian virus)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   deletion in part of the bipartite p53 binding dom…

   Promoter

   Available Since

   Sept. 26, 2005

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.1/Myc-His(-)-HSulf-2 deltaCC

    Plasmid

    #13006

    Depositor

    Steven Rosen

    Article

    Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

    Insert

    human sulfatase 2 deltaCC (SULF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Cysteine 88 to Alanine

    Promoter

    Available Since

    Oct. 30, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA-TO-HA-SH3BP1 R312A (RNAi-res)

    Plasmid

    #136646

    Purpose

    Expression of SH3BP1R12A in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUC

    Depositor

    Karl Matter, Maria Balda

    Article

    Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

    Insert

    SH3BP1R312A (RNAi resistant) (SH3BP1 Human)
    

    Use

    Tags

    HA (MQDLPGNDNSTAG)

    Expression

    Mammalian

    Mutation

    GATGACAGCCACCCACTTC changed to AATGACAGCAACACACTT…

    Promoter

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5 FRT TO myc Separase C2029A

    Plasmid

    #59822

    Purpose

    Allows the integration of myc Separase C2029A in the genome and Tet-inducible expression

    Depositor

    Stephen Taylor

    Article

    Holland et al J Cell Sci. 2006 Aug 15;119(Pt 16):3325-

    Insert

    Separase (ESPL1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    C2029A

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. Cyan Nano-lantern(Ca2+)/pcDNA3

    Plasmid

    #71344

    Purpose

    Express Cyan Nano-lantern-based luminescent Ca2+ indicator in mammalian cell

    Depositor

    Takeharu Nagai

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Cyan Nano-lantern(Ca2+)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3.Flag.HERC2-HECT-C4762A.6xHis

    Plasmid

    #39228

    Depositor

    Aziz Sancar

    Article

    Kang et al Nucleic Acids Res. 2011 Apr;39(8):3176-8

    Insert

    HERC2 HECT Domain (HERC2 Human)
    

    Use

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Mutation

    C4762A

    Promoter

    T7

    Available Since

    July 31, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA4TO-24xGCN4_v4-kif18b-Xbp1-24xPP7

    Plasmid

    #74931

    Purpose

    Translation reporter with Xbp1 pause site

    Depositor

    Marvin Tanenbaum

    Article

    Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

    Insert

    24xGCN4_v4-kif18b-Xbp1-24xPP7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5-FRT-TO-Nsp8-Nsp7-HF_GC3opt

    Plasmid

    #157702

    Purpose

    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp8-Nsp7-HF_GC3opt (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized; gene expression was optimized by…

    Promoter

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. XE257 pCDNA3.1(zeo)-hDsh3-truncDEP

    Plasmid

    #16752

    Depositor

    Randall Moon

    Article

    Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

    Insert

    FLAG-hDsh3-trunc before DEP (DVL3 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    truncated before DEP

    Promoter

    Available Since

    March 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. XE177 Xenopus Wnt-8-myc-pcDNA3

    Plasmid

    #16863

    Depositor

    Randall Moon

    Insert

    Wnt-8 (wnt8a.L Frog)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 14, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA5FRT/FLAG-Ino80 ΔN (267-1556)

    Plasmid

    #29440

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

    Insert

    INO80 (INO80 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    INO80 267-1556 (a.a.)

    Promoter

    Available Since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA 3.1 (+) FLAG-Ago2 S387A

    Plasmid

    #60652

    Purpose

    Expresses CMV-FLAG-Ago2 (N-term tag) S387A mutant in mammalian cells

    Depositor

    Carl Novina

    Article

    Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

    Insert

    Argonaute 2 (AGO2 Human)
    

    Use

    Tags

    CMV-FLAG-Ago2 (N-term tag)

    Expression

    Mutation

    S387A mutant

    Promoter

    CMV

    Available Since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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