173,841 results

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CL20_mEGFP_TMPRSS2_ERG

Plasmid

#205943

Purpose

Express mEGFP-tagged fusion protein, TMPRSS2_ERG from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

TMPRSS2_ERG (TMPRSS2, ERG Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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PB-tetO-hNIL

Plasmid

#197089

Purpose

PiggyBac plasmid with tet-inducible expression of transcription factors for motor neuron differentiation

Depositor

Brian Wainger

Article

Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

Insert

NGN2-P2A-ISL1-T2A-LHX3 (NEUROG2, ISL1, LHX3 Human)

Expression

Mammalian

Promoter

TRE3G

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-PKCdelta-EGFP

Plasmid

#178856

Purpose

Mammalian expression of PKCδ fused to EGFP

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

PKCδ (PRKCD Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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hSyn-PreSynBar-hSyn-PreRNA barcode landing pad

Plasmid

#247717

Purpose

The set of SynBar constructs used in the optimized connectome-seq experiments

Depositor

Boxuan Zhao

Article

Chen et al Nat Methods. 2026 Mar 12. doi: 10.1038/s

Insert

PreSynBar and PreRNA

Use

AAV

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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hSyn-Cre-hSyn-PreRNA barcode landing pad (PPIB)

Plasmid

#247718

Purpose

The set of RNA constructs used to produce barcode library in the optimized connectome-seq experiments. Also used for Basescope experiment.

Depositor

Boxuan Zhao

Article

Chen et al Nat Methods. 2026 Mar 12. doi: 10.1038/s

Insert

Cre and PreRNA

Use

AAV

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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hSyn-DIO-PostSynBar-P2A-mCherry

Plasmid

#247719

Purpose

The set of SynBar constructs used in the optimized connectome-seq experiments.

Depositor

Boxuan Zhao

Article

Chen et al Nat Methods. 2026 Mar 12. doi: 10.1038/s

Insert

PostSynBar and mCherry

Use

AAV

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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hSyn-Cre-hSyn-PostRNA barcode landing pad (dapB)

Plasmid

#247720

Purpose

The set of RNA constructs used to produce barcode library in the optimized connectome-seq experiments. Also used for Basescope experiment.

Depositor

Boxuan Zhao

Article

Chen et al Nat Methods. 2026 Mar 12. doi: 10.1038/s

Insert

Cre and PostRNA

Use

AAV

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pHS0570 pET45b(+) T7/LacO His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

Plasmid

#176534

Purpose

Bacterial expression of His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

CRISPR-associated IscB (Delaware Bay metagenome)

Tags

14xHis-MBP-bdSUMO and TwinStrep

Expression

Bacterial

Promoter

T7

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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NMUR1-Tango

Plasmid

#66446

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

NMUR1 (NMUR1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-D50 lamin A

Plasmid

#17653

Depositor

Tom Misteli

Article

Scaffidi et al Nat Med. 2005 Apr . 11(4):440-5.

Insert

D50 lamin A (LMNA Human)

Tags

EGFP

Expression

Mammalian

Mutation

Delta 50 (50 aa internal deletion in globular tai…

Promoter

CMV

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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7b sgRNA for EJ7-GFP reporter

Plasmid

#113624

Purpose

sgRNA/CAS9 expression plasmid to induce the 3’ double-strand break in the EJ7-GFP reporter

Depositor

Jeremy Stark

Article

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

7b sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only
pFUW-tetO-Irf8

Plasmid

#139838

Purpose

doxycycline-inducible expression of mouse Irf8 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Irf8 (Irf8 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag C/EBPb LAP

Plasmid

#66979

Purpose

Expression plasmid for mouse C/EBPb LAP; adds Flag tag to the N-terminus of protein

Depositor

Christopher Vakoc

Article

Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

Insert

C/EBP beta (Cebpb Mouse)

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

July 15, 2015

Availability

Academic Institutions and Nonprofits only
pCG-TPMV Fd32

Plasmid

#163964

Purpose

Expression of TPMV F glycoprotein in mammalian cells

Depositor

Jakob Reiser

Article

Argaw et al Mol Ther Methods Clin Dev. 2021 Apr 24;2

Insert

TPMV F glycoprotein

Expression

Mammalian

Mutation

Lacking 32 amino acids at C-terminus

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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ATP1A1_G6_T804N_v1_Dual_epegRNA_tevopreQ1

Plasmid

#187458

Purpose

Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 T804N-G6 epegRNA (tevopreQ1) with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmid

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G6 T804N epegRNA (tevopreQ1) (ATP1A1 Human)

Use

CRISPR; Prime editing

Expression

Mammalian

Promoter

Tandem U6 promoters

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLifeact-mScarlet3-H (mYongHong)

Plasmid

#239415

Purpose

Expresses Lifeact-mScarlet3-H (aka mYongHong) in mammalian cells

Depositor

Zhifei Fu

Article

Xiong et al Nat Methods. 2025 Apr 17. doi: 10.1038/s

Insert

Lifeact-mScarlet3-H

Expression

Mammalian

Mutation

M163H in mScarlet3

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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MLV Gag-CFP

Plasmid

#1812

Depositor

Walther Mothes

Article

Sherer et al Traffic 2003 Nov;4(11):785-801.

Insert

Gag (gag MuLV)

Tags

CFP

Expression

Mammalian

Available Since

May 19, 2005

Availability

Academic Institutions and Nonprofits only

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BASU RaPID plasmid

Plasmid

#107250

Purpose

Expresses the BASU RaPID labeling protein

Depositor

Paul Khavari

Article

Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

Insert

BASU

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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CROPseq-Guide-EFS-SpCas9-P2A-EGFP

Plasmid

#99248

Purpose

single-vector system with EGFP reporter for use in CROPseq knockout screens

Depositor

Alex Hewitt

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EFS

Available Since

Nov. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pX330-gRNA

Plasmid

#158973

Purpose

A human codon-optimized SpCas9 and chimeric guide RNA (gRNA3) expression plasmid.

Depositor

Charles P. Lai

Article

Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

Insert

gRNA3

Expression

Mammalian

Promoter

U6

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCR8-attL5-pegRNA-attR3

Plasmid

#213054

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attR3

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Expression

Bacterial

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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2HA-pDEST-C

Plasmid

#118374

Purpose

Gateway compatible mammalian expression vector with C-terminal 2HA to tag proteins of interest.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

2HA

Expression

Mammalian

Available Since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-ChR2(H134R)-eYFP

Plasmid

#127090

Purpose

Cre-dependent AAV expression of humanized ChR2 (with H134R mutation) fused to eYFP for optogenetic activation

Depositor

Viviana Gradinaru

Article

Rajendran et al Nat Commun. 2019 Apr 26;10(1):1944. doi:

Has Service

AAV PHP.eB

Insert

Channelrhodopsin-2

Use

AAV

Tags

eYFP (C terminal on insert)

Expression

Mammalian

Mutation

Humanized ChR2 gene with histidine 134 changed to…

Promoter

CAG

Available Since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-Dcp1a

Plasmid

#153972

Purpose

Fluorescent protein fused to mRNA decapping enzyme 1a

Depositor

Gia Voeltz

Article

Lee et al Science. 2020 Jan 31;367(6477). pii: 367

Insert

mRNA decapping enzyme 1a (DCP1A Human)

Tags

pAcGFP-C1

Expression

Mammalian

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCR8-attL1-pegRNA-attR5

Plasmid

#213053

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR5

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Expression

Bacterial

Promoter

35S-CmYCLV-AtU6

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pET21a-GluRS-His

Plasmid

#124109

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Glutamyl-tRNA ligase (gltX Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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mGLDC

Plasmid

#72881

Purpose

overexpression

Depositor

David Sabatini

Article

Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

Insert

Gldc (Gldc Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pQTEV-PSMD10

Plasmid

#31332

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

PSMD10, proteasome (prosome, macropain) 26S subunit, non-ATPase, 10 (gankyrin) (PSMD10 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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mCherry-7aa-ActA-pEGFP-C1

Plasmid

#177406

Purpose

To express mCherry fused to mitochondria outer membrane targeting sequence "ActA". mCherry faces the cytosol.

Depositor

David Andrews

Article

Aranovich et al Mol Cell. 2012 Mar 30;45(6):754-63. doi:

Insert

ActA tail anchor (actA Listeria monocytogenes)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pH2B-mScarlet3-H (aka mYongHong)

Plasmid

#239404

Purpose

Expresses H2B-mScarlet3-H in mammalian cells

Depositor

Zhifei Fu

Article

Xiong et al Nat Methods. 2025 Apr 17. doi: 10.1038/s

Insert

H2B (H2BC21 Human)

Tags

mScarlet3-H (aka mYongHong)

Expression

Mammalian

Mutation

M163H in mScarlet3

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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