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Showing: 9061 - 9090 of 13159 results

  3. 1a. pETM11-SUMO-SNCA-GFP

    Plasmid
    #107292
    Purpose
    Plasmid for the expression of the construct alpha-Synuclein-GFP, which contains N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility
    Depositor
    Dmytro Yushchenko
    Article
    Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191
    Insert
    SNCA-GFP (SNCA Human)
    Use
    Tags
    His-tag and SUMO-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    107292-plasmid-map-sequence-id-209472

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  8. K785R Smarca4-V5 IRES-puro

    Plasmid
    #107059
    Purpose
    Expression of dominant-negative K785R Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.
    Depositor
    Courtney Hodges, Jerry Crabtree
    Article
    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7
    Insert
    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    Use
    Lentiviral
    Tags
    IRES-puroR and V5
    Expression
    Mammalian
    Mutation
    K785R
    Promoter
    CAG
    Available since
    April 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    107059-plasmid-map-sequence-id-201961

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  9. pPbcas13b

    Plasmid
    #89906
    Purpose
    Bacterial expression for pbCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.
    Depositor
    Feng Zhang
    Article
    Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.
    Insert
    Cas13b
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Lac
    Available since
    May 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    89906-plasmid-map-sequence-id-178224

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  15. pAWG-dCas9-VPR

    Plasmid
    #63802
    Purpose
    SP-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; drosophila vector
    Depositor
    George Church
    Article
    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm
    Insert
    SP-dCas9-VPR
    Use
    CRISPR and Synthetic Biology
    Tags
    VPR (VP64-p65-Rta)
    Expression
    Insect
    Mutation
    Promoter
    act5c
    Available since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only

  21. pCX0393

    Plasmid
    #205984
    Purpose
    Ga0116245_10002768 T7/LacO-Cas6 + Cas14-TwinStrep + Cas7 + His14-Cas5::pJ23119-truncated DR-truncated Fn spacer
    Depositor
    Feng Zhang
    Article
    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.
    Inserts
    Ga0116245_10002768 candidate type VII proteins
    Cas14
    Cas5
    truncated CRISPR array
    Use
    Tags
    14xHis and TwinStrep-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 (proteins), pJ23119 (crRNA)
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    205984-plasmid-map-sequence-id-416238

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  28. pHRIG-PRAS40

    Plasmid
    #53599
    Purpose
    lentiviral expression of constitutively active PRAS40 and EGFP
    Depositor
    Heng Zhao
    Article
    Xiong et al Neurobiol Dis. 2014 Jun;66:43-52. doi: 1
    Insert
    PRAS40 (AKT1S1 Human)
    Use
    Lentiviral
    Tags
    IRES-EGFP and myc
    Expression
    Mammalian
    Mutation
    constitutively active (myristoylated version)
    Promoter
    CMV
    Available since
    Sept. 8, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 9061 - 9090 of 13159 results