-
Plasmid#10831DepositorInsertArf6 T27N (ARF6 Human)
UseTagsHAExpressionMammalianMutationT27N. Defective in GTP binding. Dominant negati…PromoterAvailable sinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Alpha-5-Integrin-12
Plasmid#54970PurposeLocalization: Focal Adhesions, Excitation: 587, Emission: 610DepositorInsertAlpha-5 Integrin (ITGA5 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAC-EHER
Plasmid#53262PurposeContains a 7 gene carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin diglucoside in Escherichia coliDepositorInsertcrtE, idi, crtX, crtY, crtI, crtB, crtZ
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRH030
Plasmid#159633PurposeCRISPR‐Cas9 tool for the genetic engineering of Bacillus phagesDepositorTypeEmpty backboneUseCRISPR; Shuttle vectorTagsExpressionMutationPromoterConstitutive tracrRNAAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 Raptor GFP
Plasmid#112744Purposeexpress Raptor-GFPDepositorInsertRaptor-GFP (RPTOR Human)
UseLentiviralTagseGFPExpressionMutationPromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 polylinker
Plasmid#164143PurposeLentiviral expression vector pLJM1 with extended polylinkerDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.Flex.GCaMP6f.WPRE.SV40
Plasmid#100833PurposeAAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from Synapsin promoterDepositorHas ServiceAAV1, AAV5, and AAV9InsertGCaMP6f
UseAAVTagsExpressionMammalianMutationGCaMP3-T302L R303P A317E D380Y T381R S383T R392GPromoterSynapsinAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB-CMV-MCS-Puro cPLA2-EGFP
Plasmid#162568PurposeUsed as a measure of cPLA2 activation in live cells with an EGFP tag.DepositorInsertcytosolic phospholipase a2 (pla2g4aa Zebrafish)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHSP02
Plasmid#117259PurposeGenome editing for Lactobacillus plantarum WCSF1DepositorInsertCas9, promotor P11-guide-RNA, homologous arms of Lp_0537
UseOtherTagsExpressionMutationPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
Sth1Cas9n cytosine base editor
Plasmid#194209PurposeSth1Cas9-D9A-nickase_rAPOBEC1_cytosine base editor for RNA recordingDepositorInsertSth1Cas9-D9A nickase
UseCRISPRTagsExpressionBacterialMutationChanged Aspartate 9 to Alanine.PromoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
HIP-flash (HOP-flash mutant)
Plasmid#83466PurposeNegative control for HOP-flash. 7xmut TEAD binding sites with minimal promoter plus luciferase reporter geneDepositorInsertMultimerized mutated TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-LIC
Plasmid#26094PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneUseTags6xHis and Thrombin cleavage siteExpressionBacterialMutationPromoterT7-lacO (lactose/IPTG inducible)Available sinceAug. 20, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLv-EF1a-Parkin-A92mKO2
Plasmid#213559PurposeLentiviral vector for stable expression of Parkin, internally tagged with mKO2DepositorInsertParkin (PRKN Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYLCRISPR/Cas9P35S-N
Plasmid#66191Purposeexpression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), Neo selectionDepositorInsertCas9
UseTagsExpressionPlantMutationplant-codon optimized with higher GC contents (62…Promotercauliflower mosaic virus 35S promoter (P35S)Available sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.GCaMP6s.WPRE.SV40
Plasmid#100843PurposeAAV expression of ultrasensitive protein calcium sensor from Synapsin promoterDepositorHas ServiceAAV1, AAV5, and AAV9InsertGCaMP6s
UseAAVTagsExpressionMammalianMutationGCaMP3-K78H T302L R303P D380Y T381R S383T R392GPromoterSynapsinAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 EGFP-3XNLS
Plasmid#58468PurposeExpresses a nuclear-targeted eGFP on a CMV promoterDepositorInsert3X NLS with 3X stop codons
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-LK03
Plasmid#206512PurposeAAV packaging vector containing AAV2 rep and LK03 cap and partial AAV2 p5 promoterDepositorInsertLK03 capsid
UseAAVTagsExpressionMutationPromoterp5Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-nCas9n
Plasmid#46757DepositorInsertCas9
UseCRISPRTagsSV40 NLSExpressionBacterialMutationPromoterT3Available sinceAug. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGL004
Plasmid#215336PurposeamyE integration vector for IPTG-inducible expression in Bacillus subtilis (Pspank-Hy promoter)DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterhyper-pSpankAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21a-GlnRS-His
Plasmid#124108PurposeBacterial expression plasmid for PURE system componentsDepositorInsertGlutaminyl-tRNA ligase (glnS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only