We narrowed to 10,006 results for: Gnas

Showing: 6101 - 6120 of 10006 results

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LIC-Z-YFP

Plasmid

#153544

Purpose

perform light induced optogenetic clustering of TCR ζ-Chain tagged with yellow fluorescent protein as described in the associated publication

Depositor

Katharina Gaus

Article

Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

Insert

human TCR ζ-Chain (CD247 Human)

Tags

Venus YFP

Expression

Mammalian

Mutation

with mCherry in LIC-Z replaced by YFP

Promoter

CMV

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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3xNLS-NLP-cMyc-cMyc AspCas12a

Plasmid

#182122

Purpose

pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc AspCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

3xNLS-NLP-cMyc-cMyc AspCas12a

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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RCAS-sgATG7

Plasmid

#75228

Purpose

An adaption on the RCAS/tv-a somatic cell gene transfer system, for use in combination with an existing Cas9 background in the cell/mouse of interest. Depositors: Jane Fraser/Noor Gammoh

Depositor

Noor Gammoh

Article

Fraser et al EMBO Rep. 2019 Oct 4;20(10):e47734. doi:

Insert

Autophagy Related 7 (Atg7 Mouse)

Use

Retroviral

Promoter

U6

Available Since

June 2, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)-RIα(D/D+linker)-EGFP

Plasmid

#181842

Purpose

EGFP-tagged docking/dimerization (D/D) and linker region of PKA RIα; for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

RIα-(D/D+linker)EGFP (PRKAR1A Human)

Tags

EGFP

Expression

Mammalian

Mutation

Contains only residues 12-112 of PKA RIα.

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHA_RBPMS

Plasmid

#59388

Purpose

Destination vector with RBPMS for stable cell line generation

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Tags

FLAG HA

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

Feb. 9, 2015

Availability

Academic Institutions and Nonprofits only

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CRAF_S29A_pcDNA3.1

Plasmid

#86506

Purpose

Expresses CRAF_S29A in mammalian cells [Edit]

Depositor

Dustin Maly

Article

Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

Insert

CRAF (RAF1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S29A

Promoter

CMV

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-FLCN (C terminus)

Plasmid

#72296

Purpose

overexpression

Depositor

David Sabatini

Article

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FLCN C-terminus (FLCN Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains only C terminus of FLCN as defined in PM…

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
His-GINA3

Plasmid

#74980

Purpose

Expresses His tagged GINA3 protein in bacteria.

Depositor

Pradipta Ghosh

Article

Lin et al Mol Biol Cell. 2014 Nov 5;25(22):3654-71

Insert

Rattus guanine nucleotide binding protein alpha inhibiting 3 (Gnai3) (Gnai3 )

Tags

His

Expression

Bacterial

Available Since

May 26, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-HA-MEK1 C121S

Plasmid

#53164

Purpose

Expresses GST-HA tagged human MEK1 C121S from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (MAP2K1 Human)

Tags

GST and HA

Expression

Bacterial

Mutation

C121S

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
pPGK-T7/2-CD44v8-10 (-cyt)

Plasmid

#137822

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44v8-10 (-cyt) (CD44 Human)

Tags

GFP

Expression

Mammalian

Mutation

without cytoplasmic region

Promoter

PGK

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli2 P5-6D

Plasmid

#51254

Purpose

Encodes N-3xHA-TEV-mouseGli2-C; amino acids 923,956 mutated to Asp

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2 (Gli2 Mouse)

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Mutation

amino acids 923,956 mutated to Asp

Available Since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only
pET28A-p53 (1-73) (P to A)

Plasmid

#62069

Purpose

Expression of human p53 (1-73) (all P to A) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Contains TAD residues 1-73; all Prolines changed …

Promoter

T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-hDIXDC1 S592A

Plasmid

#61223

Purpose

Mammalian expression vector encoding long isoform of human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

human DIXDC1 (DIXDC1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Changed Serine 592 to Alanine

Promoter

CMV

Available Since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pmirGLO-Calb2-3'UTR

Plasmid

#68363

Purpose

Luciferase miRNA Target Expression Vector

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Front Genet. 2017 May 29;8:70. doi: 10.3

Insert

Calb2 3'UTR (CALB2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

C to T change in UTR

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only
pSUPER-retro-puro-tetO-Mek1-shRNA

Plasmid

#53179

Purpose

Expresses shRNA against mouse Mek1 from puromycin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (Map2k1 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

June 19, 2014

Availability

Academic Institutions and Nonprofits only
His 3C Kinase dead MKK6 1-334

Plasmid

#87882

Purpose

E. coli expression of MKK6 kinase dead

Depositor

Peter Mace

Article

Weijman et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

MKK6 (MAP2K6 Human)

Tags

3C protease site and 6xHis

Expression

Bacterial

Mutation

K82A, kinase dead mutant

Promoter

T7

Available Since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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pAN19-3xFLAG-TIR1-NOSt

Plasmid

#108545

Purpose

Cloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 [Wild type] and NOS terminator

Depositor

Keiko Torii

Article

Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d

Insert

TRANSPORT INHIBITOR RESPONSE 1 fused with 3xFLAG and NOS terminator (TIR1 Mustard Weed)

Use

Cloning vector

Tags

3xFLAG

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pmirGlo-CALB2-3'UTR mir-30 mut2

Plasmid

#74429

Purpose

Calb2 UTR with mutated mir-30 site 1 and 2

Depositor

Emanuela Felley-Bosco, Rolf Stahel

Article

Kresoja-Rakic et al Front Genet. 2017 May 29;8:70. doi: 10.3

Insert

CALB2 3'UTR (CALB2 Human)

Use

Luciferase

Expression

Mammalian

Available Since

April 21, 2016

Availability

Academic Institutions and Nonprofits only

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pSico shMAPKAPK3 human

Plasmid

#85661

Purpose

expression of shRNA targeting human MAPKAPK3

Depositor

Julien Sage

Article

Reynoird et al Genes Dev. 2016 Apr 1;30(7):772-85. doi:

Insert

MAPKAPK3 (MAPKAPK3 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pECE beta1 R760A

Plasmid

#50511

Depositor

Kenneth Yamada

Article

Pankov et al J Biol Chem. 2003 May 16;278(20):18671-8

Insert

integrin beta1 (ITGB1 Human)

Expression

Mammalian

Mutation

R760A

Promoter

SV40

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only