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TLS 3: TLS.pRSET.C

Plasmid

#21829

Depositor

David Ron

Article

Crozat et al Nature. 1993 Jun 17;363(6430):640-4.

Insert

TLS (FUS Human)

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pET28b-pchA WT

Plasmid

#87102

Purpose

expresses PchA WT, the isochorismate synthase from Pseudomonas aeruginosa

Depositor

Audrey Lamb

Article

Meneely et al Arch Biochem Biophys. 2013 Oct 1;538(1):

Insert

pchA WT

Tags

his-tag

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pTXG-hTDG-K330A

Plasmid

#52282

Purpose

Bacterial expression of human TDG-K330A C-terminally fused to GyrA intein and GST

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

hTDG (TDG Human)

Tags

GyrA-intein-GST

Expression

Bacterial

Mutation

K330A, SUMO acceptor site mutation

Promoter

T7

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pBN79

Plasmid

#86717

Purpose

DamID plasmid for C. elegans

Depositor

Peter Askjaer

Article

Gonzalez-Aguilera et al Genome Biol. 2014 Feb 3;15(2):R21. doi:

Insert

Phsp16.41::Dam::MYC::Emr-1

Expression

Worm

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pUCBB-lacZa

Plasmid

#32563

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

LacZ-alpha

Use

Synthetic Biology

Tags

His

Expression

Bacterial

Promoter

Consitutive lac

Available Since

Nov. 8, 2011

Availability

Academic Institutions and Nonprofits only

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EE-SP!gIII

Plasmid

#48667

Purpose

Bacterial SP Cas9 targeting filamentous phage gene III at five protospacers

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9
anti-gIII tracrRNA

Use

CRISPR

Promoter

proC and tracrRNA promoter

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pBluescript_VEGFA-TS3_SaCas9_sgRNA2

Plasmid

#107330

Purpose

U6 driven SaCas9 sgRNA expression for VEGFA site 3

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

VEGFA-TS3 sgRNA

Use

CRISPR

Promoter

U6

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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EZH2-TALEN_Left_No_FLAG

Plasmid

#79899

Purpose

Untagged EZH2-TALEN_Left to target the N-terminus of human EZH2

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Insert

EZH2-TALEN_Left_No_FLAG

Use

TALEN

Expression

Mammalian

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pPIC-mouseKir3.2-mC-S

Plasmid

#140745

Purpose

Expression of mouse Kir3.2 fused to mCherry and Streptag II

Depositor

Arthur Laganowsky

Article

Qiao et al Biochemistry. 2020 May 6. doi: 10.1021/a

Insert

Kir3.2 (Kcnj6 Mouse)

Tags

mCherry with Streptag II

Expression

Yeast

Promoter

AOX1

Available Since

May 14, 2020

Availability

Academic Institutions and Nonprofits only

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S7_SwaI_Zeo_PacI

Plasmid

#128471

Purpose

Zeo Resistance gene

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

Zeocin Resistance gene

Expression

Bacterial

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pHaloTag-PS(RA)

Plasmid

#86627

Purpose

Expresses Akt pseudosubstrate (P-3 RA) mutant tagged with a HaloTag

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

HaloTag-PS(RA)

Expression

Mammalian

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pYC64

Plasmid

#63928

Purpose

Recipient replicative vector to construct FLAG tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

FLAG

Expression

Yeast

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pYC62

Plasmid

#63927

Purpose

Recipient replicative vector to construct HA tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

HA

Expression

Yeast

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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ROZA

Plasmid

#64198

Purpose

"Reporter of Zap-70 Activity" Fluorescent reporter of ZAP-70 tyrosine kinase activity

Depositor

Annemarie Lellouch

Article

Randriamampita et al PLoS One. 2008 Jan 30;3(1):e1521. doi: 1

Insert

ROZA

Expression

Mammalian

Promoter

CMV

Available Since

April 23, 2015

Availability

Academic Institutions and Nonprofits only
pCAGGS-Hoxc4

Plasmid

#45550

Depositor

Jeremy Dasen

Article

Lacombe et al PLoS Genet. 2013 Jan;9(1):e1003184. doi:

Insert

Hoxc4 (Hoxc4 Mouse)

Promoter

CMV / b-actin

Available Since

Aug. 5, 2013

Availability

Academic Institutions and Nonprofits only
pFA3

Plasmid

#46790

Purpose

To express ATPase inactive Fun30 in budding yeast

Depositor

Patrick Varga-Weisz

Article

Neves-Costa et al PLoS One. 2009 Dec 1;4(12):e8111. doi: 1

Insert

FUN30 (FUN30 Budding Yeast)

Tags

V5

Expression

Yeast

Mutation

ATP-binding site mutated replaced lysin 603 by ar…

Promoter

PGAL1

Available Since

Aug. 7, 2013

Availability

Academic Institutions and Nonprofits only
p064 pCS mTRPS1 P1

Plasmid

#11035

Depositor

Ramesh Shivdasani

Article

Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

Insert

TRPS1 P1 (Trps1 Mouse)

Use

Xenopus, mammalian, avian, and zebrafish

Mutation

Truncation at aa338 to mimic C338X mutation found…

Available Since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only
GST-Smad3-MH1

Plasmid

#16493

Depositor

Bert Vogelstein

Article

Zawel et al Mol Cell. 1998 Mar . 1(4):611-7.

Insert

Smad3 (SMAD3 Human)

Tags

GST

Expression

Bacterial

Mutation

amino acids 1-200 of Smad3

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pSG 413

Plasmid

#63859

Purpose

Detect translation initiation from CUG start codon (E. coli lacZ)

Depositor

Albert Dahlberg

Article

O'Connor et al Nucleic Acids Res. 1997 Mar 15;25(6):118

Insert

lacZ (lacZ Escherichia coli)

Expression

Bacterial

Mutation

CUG start codon

Promoter

PtacI

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
p3E-mCherry-HA no-pA

Plasmid

#82592

Purpose

3' entry vector with HA epitope fused to C-terminus of mCherry without pA for imaging, epitope labeling/purification

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

mCherry-HA (no pA)

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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