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3396 pBM272 Bax

Plasmid

#8770

Depositor

Stanley Korsmeyer

Article

Gross et al Mol Cell Biol 2000 May;20(9):3125-36.

Insert

Bax (Bax Mouse)

Expression

Yeast

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(-) FLIPQTV3.0 2m

Plasmid

#63731

Purpose

FLIPQTV3.0 glutamine sensor with R75MW220A mutations

Depositor

Sakiko Okumoto

Article

Gruenwald et al PLoS One. 2012;7(6):e38591. doi: 10.1371

Insert

FLIPQTV3.0 sensor, 2mM affinity

Expression

Mammalian

Available Since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pAG244 iFAST

Plasmid

#130809

Purpose

Expresses iFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Biochemistry. 2018 Oct 2;57(39):5648-565

Insert

iFAST

Tags

His-tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1-HiBiT

Plasmid

#198310

Purpose

Loads HiBiT-tagged Pdp1 into PVCs; for payload loading assays; also contains Pnf

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1-HiBiT

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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Rtn2b-mCherry

Plasmid

#186600

Purpose

Encodes human full-length Reticulon 2 isoform B fused to mCherry

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B (RTN2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pJB166

Plasmid

#86998

Purpose

CRISPR/Cas9 in fission yeast using fluoride selection

Depositor

Julien Berro

Article

Fernandez et al Yeast. 2016 Oct;33(10):549-557. doi: 10.

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-Fon/ConN2cG

Plasmid

#234709

Purpose

pAAV transfer plasmid with Ef1a promoter driving Cre- and Flp-dependent expression of G protein for rabies N2c delta G

Depositor

David Ng

Article

Mendelsohn et al bioRxiv [Preprint]. 2024 Sep 18:2024.08.

Insert

Fon Con N2cG

Use

AAV

Expression

Mammalian

Promoter

Ef1a

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5-AP-Ub-dGG

Plasmid

#232581

Purpose

Express Acceptor Peptide (AP) tagged Ubiquitin lacking the two terminal glycines

Depositor

Sagar Bhogaraju

Article

Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

Insert

Ubiquitin

Tags

AviTag

Expression

Mammalian

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-hRabin8 WT

Plasmid

#26726

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

Rabin8 (RAB3IP Human)

Tags

EGFP

Expression

Mammalian

Mutation

WT

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
pBSV2G_PflaB-mCherryBb

Plasmid

#118230

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mCherry

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven mCherry

Expression

Bacterial

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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RV-mCD4-HH5-1

Plasmid

#124961

Purpose

Express the Helicobacter hepaticus specific TCRa/b HH5-1 in T cell hybridoma cells

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH5-1

Use

retroviral

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pEX-A-U6-gRNA

Plasmid

#65626

Purpose

Empty vector for the expression U6 driven gRNA

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

U6-gRNA

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pFN18A-HaloTag-SpyCatcher

Plasmid

#206041

Purpose

HaloTag-Spy0128-(Protein of Interest)-Spy0128-SpyCatcher-8xHisTag

Depositor

Sergi Garcia-Manyes

Article

Tapia-Rojo et al Nat Protoc. 2024 Mar 11. doi: 10.1038/s4

Type

Empty backbone

Tags

HaloTag and SpyCatcher-8His

Expression

Bacterial

Promoter

T7

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pWS5321-CRISPRai-Markerless

Plasmid

#182828

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - markerless

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pFBP_promoter_acceptor

Plasmid

#189920

Purpose

Binary vector to accept a custom promoter sequence to drive fungal luciferase expression by Agrobacterium-mediated transient transformation

Depositor

Adam Steinbrenner

Article

Garcia et al Mol Plant Microbe Interact. 2023 Mar;36(

Type

Empty backbone

Expression

Plant

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCR8-attL5-pegRNA-attR3

Plasmid

#213054

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attR3

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Expression

Bacterial

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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EcpH-TM

Plasmid

#85389

Purpose

Expresses ecliptic pHluorin–ICAM-1 (intercellular adhesion molecule 1) in mammalian cells. Incorporates into viral membrane to label pseudoviruses

Depositor

Gregory Melikyan

Article

Miyauchi et al Biochem J. 2011 Mar 15;434(3):559-69. do

Insert

ecliptic pHluorin (EcpH) fused to N-terminus of human ICAM-1 transmembrane domain

Tags

3x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 23, 2017

Availability

Academic Institutions and Nonprofits only

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MET-HYGRO

Plasmid

#218113

Purpose

Integration backbone for the MET15 integration site of S. cerevisiae. Includes a HygromycinR gene and a sfGFP drop-out.

Depositor

Carl Denard

Article

Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pHEA

Plasmid

#168297

Purpose

Bacterial surface display of polypeptides (nanobodies) in E. coli

Depositor

Luis Ángel Fernández

Article

Marin et al J Bacteriol. 2010 Nov;192(21):5588-602.

Insert

Enterohemorraghic E. coli autotransporter EhaA C-terminal fragment (C-EhaA)

Tags

6xHis, E-tag, and PelB signal peptide

Expression

Bacterial

Promoter

LacI-Plac

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v2 sgATG7

Plasmid

#211534

Purpose

Deletes ATG7

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgATG7

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pMUT2-pFF-41.5-mCherry

Plasmid

#192863

Purpose

pMUT2 based vector for sensing oxygen in E. coli Nissle 1917

Depositor

Morten Sommer

Article

Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

Insert

Oxygen inducible promoter

Expression

Bacterial

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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GpACR1-pcDNA3.1-mScarlet

Plasmid

#204964

Purpose

Expression of GpACR1 in fusion with mScarlet in mammalian cells

Depositor

Peter Hegemann

Article

Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

Insert

GpACR1

Tags

mScarlet

Expression

Mammalian

Promoter

CMV (+ enhancer)

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pET28 Set9 wt

Plasmid

#24082

Depositor

Danny Reinberg

Article

Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

Insert

Set9 (SETD7 Human)

Tags

FLAG and His

Expression

Bacterial

Available Since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA Set9 mutant

Plasmid

#24085

Depositor

Danny Reinberg

Article

Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

Insert

Set9 (SETD7 Human)

Tags

FLAG

Expression

Mammalian

Mutation

H297A

Available Since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
pFlagCMV2-UbcH13

Plasmid

#12460

Depositor

Dong-Er Zhang

Article

Zou et al Biochem Biophys Res Commun. 2005 Oct 14.

Insert

Ubc13 (UBE2N Human)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GluA2-scFv-mNeon-KDEL

Plasmid

#230057

Purpose

AAV plasmid that expresses a a single chain variable fragment (scFv) for anti- GluA2 with a C-terminal mNeon. Encodes an ER retrieval motif (KDEL) at C-terminus of mNeon.

Depositor

Matthew Kennedy

Insert

Anti-GluA2 single chain variable fragment (scFv)

Use

AAV

Tags

ER retrieval motif (KDEL) and mNeon

Expression

Mammalian

Promoter

human synapsin

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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scFv-GCN4-sfGFP-deadTET1CD

Plasmid

#184442

Purpose

Catalytically DEAD TET1CD with scFv against GCN4

Depositor

Rhys Allan

Article

Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

Insert

Catalytically DEAD TET1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pRS423-CUP1-His-Ubi

Plasmid

#99541

Purpose

Yeast episomal vector for expression of His-tagged ubiquitin; HIS3 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

UBI3(1-76) (RPL40A Budding Yeast)

Tags

7xHis

Expression

Yeast

Promoter

CUP1

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV5-HA-mTmod3-WT

Plasmid

#70752

Purpose

N-terminal-HA-tagged mouse Tropomodulin3, WT

Depositor

Weiping Han

Article

Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1

Insert

Tropomodulin3 (Tmod3 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pYZ1389

Plasmid

#215348

Purpose

Nla CRISPRi Delivery Vector

Depositor

Hank Seifert

Article

Geslewitz et al mBio. 2023 Dec 21:e0302523. doi: 10.1128

Insert

Nla CRISPRi Genes (Cas5, Cas8c, Cas7)

Use

CRISPR; Nla i delivery vector

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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