We narrowed to 7,184 results for: RAP

1. pTet-O-Ngn2-puro

   Plasmid

   #52047

   Purpose

   3rd generation lentiviral vector, expresses Ngn2 and puromycin resistent gene under control of TetON promoter

   Depositor

   Marius Wernig

   Article

   Zhang et al Neuron. 2013 Jun 5;78(5):785-98. doi: 10

   Insert

   Ngn2 (Neurog2 Mouse)
   

   Use

   Lentiviral

   Tags

   T2A-and puromycin resistant cassette

   Expression

   Mammalian

   Mutation

   Promoter

   tet-on

   Available since

   April 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDH-EF1a-eFFly-mCherry

   Plasmid

   #104833

   Purpose

   3rd generation lentiviral vector. eFFly luciferase for in vivo Imaging, mCherry for fluorescent detection

   Depositor

   Irmela Jeremias

   Article

   Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

   Inserts

   eFFly
   mCherry
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-TetO-hNGN2-eGFP-Puro

   Plasmid

   #79823

   Purpose

   Expresses human NEUROGENIN2 (hNGN2), eGFP and puromycin resistance gene under control of TetON promoter. This 3rd generation lentiviral vector is used to generate NGN2-iNs from hiPSCs and hiPSC-NPCs.

   Depositor

   Kristen Brennand

   Article

   Ho et al Methods. 2015 Nov 25. pii: S1046-2023(15

   Insert

   hNGN2-P2A-eGFP-T2A-PuroR (NEUROG2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   TetO

   Available since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLY092b-lenti-EFS-BCMA-BBz-T2APRODH2-T2APuro-WPRE

   Plasmid

   #192193

   Purpose

   Lenti-BCMA-CAR-PRODH2

   Depositor

   Sidi Chen

   Article

   Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

   Insert

   Lenti-BCMA-CAR-PRODH2 (TNFRSF17 Synthetic)
   

   Use

   Lentiviral; Mammalian expression

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EF1a-iDreV

   Plasmid

   #140136

   Purpose

   can be used to generate AAV virus that will express light-inducible site-specific iDreV recombinase

   Depositor

   Hongkui Zeng

   Article

   Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

   Has Service

   AAV PHP.eB and AAV1

   Insert

   iDreV
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIG-828_HA-GD2-28z_CAR_BATF_Retroviral

   Plasmid

   #207505

   Purpose

   This plasmid can be used to generate retrovirus.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   BATF, HA-GD2-28z_CAR (BATF Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIG-830_HA-GD2-28z_CAR_tNGFR_Retroviral

   Plasmid

   #207507

   Purpose

   This plasmid can be used to generate retrovirus.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   tNGFR, HA-GD2-28z_CAR (NGFR Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETM6-G6-vioABE-4A6-vioC

   Plasmid

   #73439

   Purpose

   Deoxyviolacein pathway (vioABEC) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE) and 4A6 (vioC) for orthogonal flux redirection.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   PG6-vioABE-P4A6-vioC
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PG6 and P4A6 (orthogonal T7-lac variants)

   Available since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pStreptagII 3C EGFR EABR

   Plasmid

   #234994

   Purpose

   For production of Extracellular Vesicles (EVs), with Epithelial Growth Factor Receptor on their surface, Strep-tag II labeled on its N-terminus

   Depositor

   Monica Gonzalez-Magaldi, Daniel Leahy

   Article

   Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

   Insert

   EGFR-EABR (EGFR Human)
   

   Use

   Tags

   Streptag-II, HRV 3C site

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHA StreptagII 3C NIS EABR

    Plasmid

    #234988

    Purpose

    For production of Extracellular Vesicles (EVs), with a Strep-tag II labeled Sodium-iodide symporter (NIS), and an HA epitope at its N-terminus on their surface

    Depositor

    Monica Gonzalez-Magaldi, Daniel Leahy

    Article

    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

    Insert

    NIS-EABR (SLC5A5 Human)
    

    Use

    Tags

    HA, Streptag-II, HRV 3C site

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-EF1a-CCreV

    Plasmid

    #140132

    Purpose

    can be used to generate AAV virus that will express fusion protein of split Cre (C-terminal) and fungal photoreceptor Vivid (VVD) monomer

    Depositor

    Hongkui Zeng

    Article

    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

    Insert

    CCreV
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-EF1a-NCreV

    Plasmid

    #140131

    Purpose

    can be used to generate AAV virus that will express fusion protein of split Cre (N-terminal) and fungal photoreceptor Vivid (VVD) monomer

    Depositor

    Hongkui Zeng

    Article

    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

    Insert

    NCreV
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W

    Plasmid

    #67980

    Purpose

    Cas9 activity reporter with BFP and GFP.

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKBFP2AGFP cassette, WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAVS1-Puro CAG CLTX-NKG2D-2B4-CD3z CAR

    Plasmid

    #157744

    Purpose

    knock CLTX-NKG2D-2B4-CD3z CAR into AAVS1 safe harbor locus for constitutive expression

    Depositor

    Xiaoping Bao

    Article

    Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

    Insert

    chlorotoxin containing chimeric antigen receptor targeting glioblastoma
    

    Use

    CRISPR, Synthetic Biology, and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-MH6-Bsu

    Plasmid

    #163911

    Purpose

    Expresses Bsu large fragment for affinity purification

    Depositor

    Paul Freemont

    Article

    Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

    Insert

    Bsu polymerase
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-MH6-uvsY

    Plasmid

    #163914

    Purpose

    Expresses uvsY for bacterial expression and affinity purification

    Depositor

    Paul Freemont

    Article

    Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

    Insert

    uvsY (uvsY Enterobacteria phage T4)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. SARS-CoV-2 Nucleocapsid-3xHA-IRES-mcherry

    Plasmid

    #173030

    Purpose

    To express SARS-CoV-2 Nucleocapsid protein tagged with 3xHA and in frame with mcherry (NCP-IRES-mcherry) in mammalian cells

    Depositor

    Mohamed Fareh

    Article

    Fareh et al Nat Commun. 2021 Jul 13;12(1):4270. doi:

    Insert

    SARS-CoV-2 Nucleocapsid phosphoprotein
    

    Use

    Lentiviral

    Tags

    3xHA and IRES-mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    Gag (truncated)

    Available since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-hATG7CS

    Plasmid

    #87868

    Purpose

    Express a active-site mutant human Atg7/Apg7 C572S in mammalian cells

    Depositor

    Isei Tanida, Eiki Kominami

    Article

    Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

    Insert

    ATG7 C572S mutant (ATG7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C572S mutant

    Promoter

    CMV

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBK1314-AAV-EFSNC-dSaCas9-KRAB-MECP2

    Plasmid

    #223159

    Purpose

    Expression of KRAB and truncated MECP2 with dSaCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB-MECP2 (MECP2 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 204-310 (MECP2)

    Promoter

    EF1a

    Available since

    Aug. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. Ef1a-pspCas13b-NES-3xFLAG-T2A-BFP

    Plasmid

    #173029

    Purpose

    For expression of cytoplasmic pspCas13b protein tagged with 3xHA-T2A-BFP for gene silencing in mamallian cells.

    Depositor

    Mohamed Fareh

    Article

    Fareh et al Nat Commun. 2021 Jul 13;12(1):4270. doi:

    Insert

    pspCas13b
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG, BFP, HIV NES, and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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