We narrowed to 10,398 results for: plasmids 101

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pJ02B2Rm_AE

Plasmid

#66065

Purpose

MoClo Transcriptional Unit: FACS Standard Color Controls - High RFP expression cassette - Kanamycin plasmid [A:J23102:B:BCD2:C:E1010m:D:B0015:E]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

FACS Controls

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pJ02B2Rm_EF

Plasmid

#66066

Purpose

MoClo Transcriptional Unit: FACS Standard Color Controls - High RFP expression cassette - Kanamycin plasmid [E:J23102:B:BCD2:C:E1010m:D:B0015:F]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

FACS Controls

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pJ02B2Rm_AE

Plasmid

#66059

Purpose

MoClo Transcriptional Unit: FACS Standard Color Controls - High RFP expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E1010m:D:B0015:E]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

FACS Controls

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-OCT4-KLF4-cMYC

Plasmid

#216175

Purpose

Generate lentiviruses encoding for OCT4, KLF4 and c-MYC (polycistronic vector); used in conjunction with SOX vectors for pluripotency reprogramming

Depositor

Ralf Jauch

Article

Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

Insert

OCT4 (POU5F1, KLF4, MYC Human)

Use

Lentiviral

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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Sox2-t2A-GFP

Plasmid

#127537

Purpose

Expresses human SOX2 and eGFP via t2A linker.

Depositor

Jennifer Mitchell

Article

Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

Insert

SOX2 (SOX2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-double floxed-Gad67-WPRE-HGHpA

Plasmid

#184632

Purpose

Expresses Gad67, driven by the Ef1a promoter, in a Cre-dependent fashion

Depositor

Nicolas Tritsch

Article

Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

Insert

Gad67 (Gad1 Mouse)

Use

AAV

Promoter

EF1a

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-mir451 EF1Alpha-puro-T2A-BFP

Plasmid

#164792

Purpose

Express miR-451 under the U6 promoter in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

microRNA 451a (MIR451A Human)
puro-T2A-BFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1Alpha and U6

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pRluc-LC3G120A

Plasmid

#105003

Purpose

Mammalian expression of Rluc-LC3G120A. Can be used for measuring autophagic flux.

Depositor

Marja Jããttelã

Article

Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

Insert

LC3B (Map1lc3b Rat)

Use

Luciferase

Tags

Rluc with C124A substitution

Expression

Mammalian

Mutation

Glycine 120 mutated to alanine, prevents lipidati…

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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AID-mTagBFP2-loxP_myo2_neoR-loxP

Plasmid

#194055

Purpose

Dual-selection cassette plasmid for knocking-in AID-mTagBFP2 into the C. elegans genome

Depositor

Kota Mizumoto

Article

Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

Insert

IAA17 (AXR3 Mustard Weed)

Tags

codon optimized mTagBFP2

Expression

Worm

Promoter

None

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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Sox2-t2A-mCherry

Plasmid

#127538

Purpose

Expresses human SOX2 and mCherry via t2A linker.

Depositor

Jennifer Mitchell

Article

Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

Insert

SOX2 (SOX2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-memRFP-3xPax7gRNA

Plasmid

#224569

Purpose

Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.

Depositor

Erica Hutchins

Article

Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

Insert

mRFP1

Use

CRISPR

Tags

Membrane localization signal

Mutation

Codon optimized

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_CaMKII_NES-his-CaMPARI2-F391W-WPRE-SV40

Plasmid

#163698

Purpose

AAV vector expressing CaMPARI2_F391W primarily in excitatory neurons in rodent cortex

Depositor

Gina Turrigiano

Article

Trojanowski et al Neuron. 2021 Feb 17;109(4):663-676.e5. d

Insert

CaMKII_NES-his-CaMPARI2-F391W-WPRE-SV40

Use

AAV

Tags

FLAG-HA-myc and NES_his

Expression

Mammalian

Promoter

CaMKII fragment

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-NES-tdTomato-LINuS-WPRE

Plasmid

#159894

Purpose

AAV expression of tdTomato fused to LINuS, a light-inducible nuclear localization signal

Depositor

Tatsushi Onaka

Article

Inutsuka et al Biochem Biophys Res Commun. 2020 Jan 29;

Insert

NES-tdTomato-LINuS

Use

AAV

Expression

Mammalian

Promoter

EF1α promoter

Available Since

Sept. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pAM90

Plasmid

#226682

Purpose

Rpn9S111TAG lid (Rpn5, MBP-HRV-Rpn6, Rpn8, Rpn9S111TAG, Rpn11)

Depositor

Andreas Martin

Article

Bard et al Cell. 2019 Apr 4;177(2):286-298.e15. doi

Inserts

Rpn5 (RPN5 Budding Yeast)
Rpn6 (RPN6 Budding Yeast)
Rpn8 (RPN8 Budding Yeast)
Rpn9 (RPN9 Budding Yeast)
Rpn11 (RPN11 Budding Yeast)

Tags

MBP-HRV

Expression

Bacterial

Mutation

S111TAG

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-GRAB_ATP1.0

Plasmid

#167579

Purpose

Express the ATP sensor GRAB_ATP1.0 in astrocytes

Depositor

Yulong Li

Article

Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

Insert

ATP sensor GRAB_ATP1.0 (P2RY1 Human)

Use

AAV

Promoter

GfaABC1D

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_ATP1.0

Plasmid

#167577

Purpose

Express the ATP sensor GRAB_ATP1.0 in neurons

Depositor

Yulong Li

Article

Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

Insert

ATP sensor GRAB_ATP1.0 (P2RY1 Human)

Use

AAV

Promoter

hSyn

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-L7-6-DD-T6B-WT-YFP

Plasmid

#235145

Purpose

Expresses the inducible T6B peptide fused with DHFR and YFP under the Purkinje cell-specific L7-6 promoter

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

DHFR-fused T6B peptide

Use

AAV

Promoter

L7-6

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-L7-6-FLAG-HA-T6B-WT-YFP

Plasmid

#235141

Purpose

Expresses the T6B peptide fused with YFP under the Purkinje cell-specific L7-6 promoter

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

T6B peptide

Use

AAV

Tags

FLAG/HA

Promoter

L7-6

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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HDM-Hgpm2

Plasmid

#204152

Purpose

Lentivirus helper plasmid coding for Gag/Pol

Depositor

Jesse Bloom

Article

Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

Insert

HIV-1 gag/pol

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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HDM-tat1b

Plasmid

#204154

Purpose

Lentivirus helper plasmid coding for Tat

Depositor

Jesse Bloom

Article

Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

Insert

tat

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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