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pMIA3-Pansio

Plasmid

#214445

Purpose

Expresses eSpCas9 and gRNA targeting Pansio-1 GSH

Depositor

Roger Foo

Article

Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

Insert

eSpCas9-P2A-mRuby2-P2A-tp53dn

Use

CRISPR

Expression

Mammalian

Promoter

EF1a

Available Since

May 16, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac1_GI.1_WT

Plasmid

#162579

Purpose

Expresses norovirus GI.1 VP1 protein in Sf9 insect cells

Depositor

Peter Kwong

Article

Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

Insert

VP1 protein from human norovirus GI.1

Expression

Insect

Promoter

polyhedrin

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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PB-CAG-DDdCas9VPH-T2A-GFP-IRES-Neo

Plasmid

#102886

Purpose

PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CAG

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pJJiaDEST-APEX2-GAL3

Plasmid

#149518

Purpose

Express APEX2-Galectin-3 in mammalian cells

Depositor

Vojo Deretic

Article

Jia et al Mol Cell. 2018 Apr 5;70(1):120-135.e8. d

Insert

LGALS3 (LGALS3 Human)

Tags

APEX2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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M3-dCAS9-DNMT3A-DNMT3L (dCas9-3A-3L)

Plasmid

#218777

Purpose

Expresses dCAS9-DNMT3A-DNMT3L fusion protein and double marker GFP-T2A-Puro under independent promoters

Depositor

Maja Jagodic

Article

Kakhki et al bioRxiv 2025.03.15.641804; doi: 10.1101/

Inserts

M3-dCAS9-DNMT3A-DNMT3L (dCas9-3A-3L)
GFP-T2A-Puro

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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PT3.5/CMV-EGFRvIII

Plasmid

#20280

Depositor

John Ohlfest

Article

Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

Insert

EGFRvII (EGFR Human)

Use

Transposon

Mutation

See depositor comments below.

Available Since

Dec. 3, 2009

Availability

Academic Institutions and Nonprofits only

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SpyCas9-E566-LOV-C450A-D432V(M519)

Plasmid

#250779

Purpose

Potent temperature-deactivated SpyCas9

Depositor

Dominik Niopek

Article

Kroell et al Nat Chem Biol. 2026 Feb 12. doi: 10.1038

Insert

SpyCas9 with an AsLOV2 insertion behind E566 and C450A and D432V mutation

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

C450A, D432V

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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Lentiviral-TOP-dGFP-reporter

Plasmid

#14715

Purpose

Beta-catenin reporter - 3rd generation self-inactivating lentiviral plasmid LEF-1/TCF-responsive promoter driven d2-eGFP (destabilized, half-life of 2h).

Depositor

Tannishtha Reya

Article

Reya et al Nature. 2003 May 22. 423(6938):409-14.

Insert

LEF-1/TCF-responsive promoter (Ctnnb1 )

Use

Lentiviral

Tags

d2 eGFP

Expression

Mammalian

Available Since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only

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M3-dCAS9-M.SssI (dCas9-M.SssI)

Plasmid

#218782

Purpose

Expresses dCAS9-M.SssI fusion protein and double marker GFP-T2A-Puro under independent promoters

Depositor

Maja Jagodic

Article

Kakhki et al bioRxiv 2025.03.15.641804; doi: 10.1101/

Inserts

M3-dCAS9-M.SssI (dCas9-M.SssI)
GFP-T2A-Puro

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNAFLAG-ATM-kd

Plasmid

#43908

Depositor

Stephen Elledge

Article

Cortez et al Science. 1999 Nov 5. 286(5442):1162-6.

Insert

ataxia telangiectasia mutated (ATM Human)

Tags

FLAG and His

Expression

Mammalian

Mutation

kinase dead (D2870A and N2875K)

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pCAGGS-4493NES

Plasmid

#138373

Purpose

Booster-PKA, a Red-Shifted Genetically Encoded FRET Biosensor for monitoring PKA activity.

Depositor

Michiyuki Matsuda

Article

Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

Insert

Booster-PKA

Expression

Mammalian

Promoter

CAG

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pJJiaDEST-APEX2-GAL8

Plasmid

#149519

Purpose

Express APEX2-Galectin-8 in mammalian cells

Depositor

Vojo Deretic

Article

Jia et al Mol Cell. 2018 Apr 5;70(1):120-135.e8. d

Insert

LGALS8 (LGALS8 Human)

Tags

APEX2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pC-FLAG-ZCD2

Plasmid

#79274

Purpose

Mammalian expression of ZCD2 with a C-terminal FLAG tag

Depositor

Rita Shiang

Article

Amr et al Am J Hum Genet. 2007 Oct;81(4):673-83. E

Insert

ZCD2 (CISD2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits
pAAV-pCALM1-SpCas9-miniU6-sgRNAShank3

Plasmid

#213973

Purpose

AAV vector to express SpCas9 driven by pCALM1 promoter for targeting Shank3 locus

Depositor

Zhonghua Lu

Article

Wang et al Cell Rep. 2023 Nov 28;42(11):113348. doi

Insert

Shank3 sgRNA

Use

AAV and CRISPR

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST-DHFR F[3]-N (LEU2)

Plasmid

#177798

Purpose

Gateway destination vector to insert genes of interest having a N-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

Type

Empty backbone

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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M3-dCAS9-SunTag (dCas9-SunTag)

Plasmid

#218783

Purpose

Expresses dCAS9-SunTag fusion protein and double marker GFP-T2A-Puro under independent promoters

Depositor

Maja Jagodic

Article

Kakhki et al bioRxiv 2025.03.15.641804; doi: 10.1101/

Inserts

M3-dCAS9-SunTag (dCas9-SunTag)
GFP-T2A-Puro

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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px-458-sgRNA-scramble

Plasmid

#206924

Purpose

Plasmid expressing Cas9, GFP and non-targeting guides for using as a control in CRISPR experiments

Depositor

José Cuezva

Article

Romero-Carraminana et al Commun Biol. 2023 Aug 12;6(1):836. doi:

Insert

non-targeting human sgRNA guides

Use

CRISPR

Promoter

U6

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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FHRE-Luc

Plasmid

#1789

Purpose

Reporter plasmid for FOXO3a. Forkhead responsive element with luciferase readout.

Depositor

Michael Greenberg

Article

Brunet et al Cell 1999 Mar 19;96(6):857-68.

Insert

FHRE (FOXO3 Human)

Use

Luciferase

Expression

Mammalian

Mutation

3 copies of FRE

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pFastbac1-3FLAG-TOP2A

Plasmid

#247921

Purpose

Insect expression construct for N-terminal 3xFLAG-tagged TOP2A

Depositor

Junjie Chen, Huimin Zhang

Article

Zhang et al Sci Adv. 2023 Dec 8;9(49):eadi6681. doi:

Insert

TOP2A (TOP2A Human)

Tags

3xFLAG

Expression

Insect

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pSF4 TetCMV intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA

Plasmid

#119945

Purpose

Expresses SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats

Depositor

Jeffrey Chao

Article

Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

Renilla luciferase

Tags

24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …

Expression

Bacterial and Mammalian

Promoter

TetCMV

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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