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Showing: 1501 - 1550 of 1666 results

  5. pGG-GTS-TurboID-GFP

    Plasmid
    #209405
    Purpose
    Transiently expressing and visualizing the subcellular localization of TurboID fused with a Golgi targeting sequence (GTS) in planta
    Depositor
    Rosa Lozano-Durán
    Article
    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1
    Insert
    GTS-3XHA-TurboID
    Use
    Tags
    GFP
    Expression
    Plant
    Mutation
    TurboID gene sequence C249A
    Promoter
    Cauliflower mosaic virus (CaMV) 35S promoter
    Available since
    Jan. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    209405-plasmid-map-sequence-id-418457

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  6. pGG-mTP-TurboID-GFP

    Plasmid
    #209404
    Purpose
    Transiently expressing and visualizing the subcellular localization of TurboID fused with a Mitochondrial transit peptide (mTP) in planta
    Depositor
    Rosa Lozano-Durán
    Article
    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1
    Insert
    mTP-3XHA-TurboID
    Use
    Tags
    GFP
    Expression
    Plant
    Mutation
    TurboID gene sequence C249A
    Promoter
    Cauliflower mosaic virus (CaMV) 35S promoter
    Available since
    Jan. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    209404-plasmid-map-sequence-id-418443

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  7. pFsynW SYT1-PGM reporter

    Plasmid
    #197278
    Purpose
    Encodes for viral expression of Synaptotagmin 1 (SYT1) for use in the RUSH system; visualized with HaloTag. Reporter only (no hook). SYT1 is mutated to disrupt palmitoylation and glycosylation.
    Depositor
    Edwin Chapman
    Article
    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755
    Insert
    SYT1 and HaloTag (Syt1 Rat)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mutation
    T15A, T16A, N24Q, C74A, C75A, C77A, C79A, and C82A
    Promoter
    hSyn (human synapsin I promoter)
    Available since
    July 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197278-plasmid-map-sequence-id-389096

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  12. FUW mCherry-GFP-LC3

    Plasmid
    #110060
    Purpose
    To visualize free autophagosomes (GFP and mCherry fluorescence) and autophagosomes that have fused with the lysosome (autolyosomes; mCherry fluorescence only, due to acid sensitivity of GFP)
    Depositor
    Anne Brunet
    Article
    Leeman et al Science. 2018 Mar 16;359(6381):1277-1283
    Insert
    mCherry-GFP-LC3
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    110060-plasmid-map-sequence-id-438935

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  21. qTAG-N-Solo-mStayGold-MYO1C

    Plasmid
    #227306
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the MYO1C locus. For membrane visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MYO1C (Addgene #227305)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    MYO1C Homology Arms flanking a mStayGold Tag (MYO1C Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227306-plasmid-map-sequence-id-452076

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  22. pEGFP-ATF6-(S1P-)

    Plasmid
    #32956
    Purpose
    For visualization of ATF6 in mammalian cells with a mutation in ATF6 that blocks cleavage by protease S1P. Activated protein will remain in the Golgi.
    Depositor
    Ron Prywes
    Article
    Chen et al J Biol Chem. 2002 Apr 12. 277(15):13045-
    Insert
    ATF6 (ATF6 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    S1Protease site mutation: R415A/R416A
    Promoter
    CMV
    Available since
    Feb. 2, 2012
    Availability
    Academic Institutions and Nonprofits only
    32956-plasmid-map-sequence-id-226826

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  24. DHFR-mNeon-GluA1

    Plasmid
    #173009
    Purpose
    For light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Intracellular trafficking can be better visualized with mNeon.
    Depositor
    Matthew Kennedy
    Article
    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212
    Insert
    DHFR-mNeon-GluA1
    Use
    Tags
    DHFR and mNeon
    Expression
    Mammalian
    Mutation
    Promoter
    Chicken beta actin (CAG)
    Available since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    173009-plasmid-map-sequence-id-339113

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  25. qTAG-N-Solo-mScarlet-TUBA1B

    Plasmid
    #207764
    Purpose
    Donor template for mScarlet insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TUBA1B Homology Arms flanking a mScarlet Tag (TUBA1B Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207764-plasmid-map-sequence-id-413790

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  26. qTAG-C-moxGFP-Puro-H2BC11

    Plasmid
    #207758
    Purpose
    Donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 17, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207758-plasmid-map-sequence-id-415316

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  27. pTol2-CreLite

    Plasmid
    #131783
    Purpose
    Tol2 destination vector carrying Ubi:CreLite with mTagBFP2 as a visual marker.
    Depositor
    George Eisenhoffer
    Article
    Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi
    Insert
    PhyBCreC, PIF6CreN, and mTagBFP2
    Use
    Cre/Lox and Synthetic Biology; Zebrafish tol2 tra…
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    Ubi
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    131783-plasmid-map-sequence-id-268078

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  28. phage - ubc - nls - 2xmcp - egfp - BirA*

    Plasmid
    #131132
    Purpose
    BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.
    Depositor
    Ralf Jansen
    Article
    Mukherjee et al Proc Natl Acad Sci U S A. 2019 Jun 25;11
    Insert
    NLS-HA-2xMCP-EGFP-BirA*
    Use
    Lentiviral
    Tags
    NLS, HA
    Expression
    Mammalian
    Mutation
    BirA* = BIrA >R118G
    Promoter
    human ubiquitin C (UBC)
    Available since
    Aug. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    131132-plasmid-map-sequence-id-255500

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  29. qTAG-C-miRFP670nano3-Blast-H2BC11

    Plasmid
    #207762
    Purpose
    Donor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Homology Arms flanking a miRFP670nano3-Blast Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207762-plasmid-map-sequence-id-416207

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  31. qTAG-C-mScarlet-Puro-H2BC11

    Plasmid
    #207761
    Purpose
    Donor template for mScarlet-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Homology Arms flanking a mScarlet-Puro Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 17, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207761-plasmid-map-sequence-id-415325

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  32. qTAG-C-moxGFP-Blast-H2BC11

    Plasmid
    #207757
    Purpose
    Donor template for moxGFP-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Homology Arms flanking a moxGFP-Blast Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 16, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207757-plasmid-map-sequence-id-415158

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  33. qTAG-C-mNeon-Blast-TUBB4B

    Plasmid
    #207786
    Purpose
    Donor template for mNeon-2A-Blast insertion into the C-terminus of the TUBB4B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBB4B Addgene #207785
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TUBB4B Homology Arms flanking a mNeon-Blast Cassette (TUBB4B Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    207786-plasmid-map-sequence-id-413796

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  35. qTAG-C-moxGFP-Puro-H2BC11-MMEJ

    Plasmid
    #207760
    Purpose
    MMEJ donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Short Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207760-plasmid-map-sequence-id-413789

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  36. pONSY-CoNMM:mCherry

    Plasmid
    #111878
    Purpose
    This plasmid expresses mCherry fluorescent protein fused to an N-Myristoylation motif (NMM) from the endogenous Src2 gene (CAOG_06360). It can be used to visualize the membrane and filopodia.
    Depositor
    Inaki Ruiz-Trillo
    Article
    Parra-Acero et al Development. 2018 May 11. pii: dev.16210
    Insert
    Capsaspora N-Myristoylation motif (CoNMM) from the Src2 gene fused to mCherry
    Use
    Capsaspora owczarzaki
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Elongation Factor 1 alpha (EF1a) from Capsaspora
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    111878-plasmid-map-sequence-id-213490

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  37. pRD398

    Plasmid
    #163105
    Purpose
    Expression of mCherry_H-NSdbd in eukaryotes for visualisation of nuclear DNA
    Depositor
    Remus Dame
    Article
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Insert
    NLS_mCherry_H-NSdbd_NLS
    Use
    Eukaryotic expression
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter; T7 promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    163105-plasmid-map-sequence-id-321846

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  38. pONSY-CoH2B:Venus

    Plasmid
    #111877
    Purpose
    This plasmid expresses Venus fluorescent protein fused to endogenous Histone H2B (H2B) gene of Capsaspora (CAOG_01818). It can be used to transfect Capsaspora cells and visualize nucleus in vivo.
    Depositor
    Inaki Ruiz-Trillo
    Article
    Parra-Acero et al Development. 2018 May 11. pii: dev.16210
    Insert
    Capsaspora Histone H2B (CoH2B) fused to Venus
    Use
    Capsaspora owczarzaki
    Tags
    Venus
    Expression
    Mutation
    Promoter
    Elongation Factor 1 alpha (EF1a) from Capsaspora
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    111877-plasmid-map-sequence-id-213489

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  39. pRD406

    Plasmid
    #163111
    Purpose
    Expression of mNeonGreen_H-NSdbd in eukaryotes for visualisation of nuclear DNA
    Depositor
    Remus Dame
    Article
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Insert
    NLS_mNeonGreen_H-NSdbd_NLS
    Use
    Eukaryotic expression
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter; T7 promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    163111-plasmid-map-sequence-id-321832

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  40. pRD399

    Plasmid
    #163106
    Purpose
    Expression of mEGFP_H-NSdbd in eukaryotes for visualisation of nuclear DNA
    Depositor
    Remus Dame
    Article
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Insert
    NLS_mEGFP_H-NSdbd_NLS
    Use
    Eukaryotic expression
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter; T7 promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    163106-plasmid-map-sequence-id-321847

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  41. pRD397

    Plasmid
    #163104
    Purpose
    Expression of mTurquoise2_H-NSdbd in eukaryotes for visualisation of nuclear DNA
    Depositor
    Remus Dame
    Article
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Insert
    NLS_mTurquoise2_H-NSdbd_NLS
    Use
    Eukaryotic expression
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter; T7 promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    163104-plasmid-map-sequence-id-321845

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  43. qTAG-C-mStayGold-Puro-H2BC11-MMEJ

    Plasmid
    #227333
    Purpose
    MMEJ Donor template for mStayGold-2A-Puro insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Short Homology Arms flanking a mStayGold-2A-Puro Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227333-plasmid-map-sequence-id-452137

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  44. qTAG-C-Solo-miRFP670nano3-H2BC11

    Plasmid
    #227331
    Purpose
    Donor template for miRFP670nano3 insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Homology Arms flanking a miRFP670nano3 Tag (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227331-plasmid-map-sequence-id-452128

    Enlarge

  45. qTAG-C-mStayGold-Puro-H2BC11

    Plasmid
    #227332
    Purpose
    Donor template for mStayGold-2A-Puro insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Homology Arms flanking a mStayGold-2A-Puro Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227332-plasmid-map-sequence-id-452132

    Enlarge

  46. qTAG-N-Solo-mStayGold-TUBA1B

    Plasmid
    #227327
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TUBA1B Homology Arms flanking a mStayGold Tag (TUBA1B Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227327-plasmid-map-sequence-id-452117

    Enlarge

  47. qTAG-C-Solo-mStayGold-H2BC11

    Plasmid
    #227330
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Homology Arms flanking a mStayGold Tag (H2BC11 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227330-plasmid-map-sequence-id-452124

    Enlarge

  48. qTAG-C-mStayGold-Puro-PXN

    Plasmid
    #227317
    Purpose
    Donor template for mStayGold-2A-Puro insertion into the C-terminus of the PXN locus. For focal adhesion visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PXN (Addgene #227315)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    PXN Homology Arms flanking a mStayGold-2A-Puro Cassette (PXN Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227317-plasmid-map-sequence-id-452087

    Enlarge

  49. qTAG-C-mStayGold-EFS-Puro-PLK4

    Plasmid
    #227311
    Purpose
    Donor template for mStayGold-EFS-Puro insertion into the C-terminus of the PLK4 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px458-PLK4 (Addgene #227310)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    PLK4 Homology Arms flanking a mStayGold-EFS-Puro Cassette (PLK4 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227311-plasmid-map-sequence-id-452079

    Enlarge

  50. qTAG-N-Solo-mStayGold-PCNT

    Plasmid
    #227285
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the PCNT locus. For pericentriolar material visualization. To be co-transfected with sgRNA plasmid px330-PCNT (Addgene #227284)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    PCNT Homology Arms flanking a mStayGold Tag (PCNT Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227285-plasmid-map-sequence-id-452054

    Enlarge

Showing: 1501 - 1550 of 1666 results