We narrowed to 16,118 results for: form

1. RAB14 Q70L

   Plasmid

   #61494

   Purpose

   to overexpress constitutively active RAB14. Vector includes GFP driven by hUbC promoter

   Depositor

   Curt Civin

   Article

   Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

   Insert

   RAB14 (RAB14 Human)
   

   Use

   Lentiviral

   Mutation

   Q70L, active form

   Promoter

   EF1a

   Available Since

   Jan. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pET15-MTFmt

   Plasmid

   #31174

   Depositor

   Linda Spremulli

   Article

   Takeuchi et al J Biol Chem. 1998 Jun 12. 273(24):15085-

   Insert

   Mature form bovine mitochondrial methionyl-tRNA transformylase (MTFMT Bovine)
   

   Tags

   His and thrombin cleavage

   Expression

   Bacterial

   Available Since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pLV-SFFV-RAB10-WPRE-UbC-Emerald

   Plasmid

   #203804

   Purpose

   Lentiviral vector plasmid expressing human RAB10 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Bart Nieuwenhuis

   Inserts

   RAB10 (RAB10 Human)
   Emerald
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSP64T tBMPR (DM#135)

   Plasmid

   #15067

   Depositor

   Douglas Melton

   Article

   Graff et al Cell. 1994 Oct 7. 79(1):169-79.

   Insert

   BMP receptor (bmpr1a Frog)
   

   Expression

   Bacterial

   Mutation

   Truncated dominant negative form

   Available Since

   Oct. 18, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pAPM sh2 DNMT1

   Plasmid

   #88885

   Purpose

   Lentiviral vector expressing shRNA against murine DNMT1

   Depositor

   Silvia Monticelli

   Article

   Leoni et al Proc Natl Acad Sci U S A. 2017 Feb 6. pi

   Insert

   shRNA no.2 DNMT1
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV (spleen focus forming virus)

   Available Since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pT-35

   Plasmid

   #16092

   Depositor

   Yoshiko Kikuchi

   Article

   Takahashi et al J Biol Chem. 2001 Dec 28. 276(52):48973-

   Insert

   SMT3 (SMT3 Budding Yeast)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pAPM sh1 DNMT1

   Plasmid

   #88884

   Purpose

   Lentiviral vector expressing shRNA against murine DNMT1

   Depositor

   Silvia Monticelli

   Article

   Leoni et al Proc Natl Acad Sci U S A. 2017 Feb 6. pi

   Insert

   shRNA no.1 DNMT1
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV (spleen focus forming virus)

   Available Since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. WT-short-UBASH3A-V5-6xHis

   Plasmid

   #192102

   Purpose

   Mammalian expression of human wild-type short-form UBASH3A with V5-6xHis tag

   Depositor

   Patrick Concannon

   Article

   Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

   Insert

   UBASH3A (UBASH3A Human)
   

   Tags

   V5, 6xHis

   Expression

   Mammalian

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDEST-Hemmar-FLP(D)

   Plasmid

   #119895

   Purpose

   FLP(D) with a hsp70 promoter

   Depositor

   Rita Sousa-Nunes

   Article

   Chai et al Elife. 2018 Nov 27;7. pii: 38393. doi: 1

   Insert

   FLP(D)
   

   Use

   Drosophila expression

   Mutation

   mutated form of FLP, which at position 5 contains…

   Available Since

   Feb. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. XAC1 S3E

    Plasmid

    #245947

    Purpose

    Expressing non-actin binding form of XAC1 (phosphomimetic)

    Depositor

    James Bamburg

    Article

    Dawe et al Curr Biol. 2003 Feb 4;13(3):252-7. doi:

    Insert

    cofilin 1 (cfl1) (cfl1.L Frog)
    

    Use

    Frog expression

    Mutation

    S3E

    Promoter

    CMV

    Available Since

    Oct. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLV-SFFV-ZFYVE27 S14D S25D S32D T261D-WPRE-UbC-Emerald

    Plasmid

    #214468

    Purpose

    Lentiviral vector plasmid expressing human ZFYVE27 mutations S14D S25D S32D T261D under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Inserts

    ZFYVE27 (ZFYVE27 Human)
    Emerald
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    S14D S25D S32D T261D

    Available Since

    July 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-MF3958 Heavy chain-SpyTag003

    Plasmid

    #216303

    Purpose

    Expresses anti-HER2 MF3958 Heavy chain-SpyTag003 in mammalian cells. To be paired with MF3958 light chain to form the anti-HER2 MF3958 Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    MF3958 Heavy chain-SpyTag003
    

    Use

    Affinity Reagent/ Antibody

    Tags

    His6 tag, Signal peptide, and SpyTag003

    Expression

    Mammalian

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1-H2-18 Light chain

    Plasmid

    #216308

    Purpose

    Expresses anti-HER2 H2-18 Light chain in mammalian cells. To be paired with H2-18 heavy chain to form the anti-HER2 H2-18 Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    H2-18 Light chain
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Signal peptide

    Expression

    Mammalian

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-39S Heavy chain-SpyTag003

    Plasmid

    #216296

    Purpose

    Expresses anti-HER2 39S Heavy chain-SpyTag003 in mammalian cells. To be paired with 39S light chain to form the anti-HER2 39S Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    39S Heavy chain-SpyTag003
    

    Use

    Affinity Reagent/ Antibody

    Tags

    His6 tag, Signal peptide, and SpyTag003

    Expression

    Mammalian

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDEST14-Cys-SpyCatcher (Min)

    Plasmid

    #216713

    Purpose

    Expresses truncated minimal form of SpyCatcher with free cysteine for labeling in bacteria. See also Li...Howarth, J Mol. Biol., 2014

    Depositor

    Alex Dunn

    Article

    Zhong et al Cell Rep Methods. 2022 Sep 2;2(9):100278

    Insert

    Cys-SpyCatcher (Min)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    See Li...Howarth, J. Mol. Biol., 2014

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDGB3_alpha2-p35S:HR1bR181/185A -GFP-gRHD6-UTR-Ter

    Plasmid

    #211847

    Purpose

    Plant transient expression cassette for RHD6 fused with GFP and a mutant form of the HR1b degron

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    HR1b
    

    Use

    Synthetic Biology

    Tags

    GFP

    Expression

    Plant

    Mutation

    R181A R185A

    Promoter

    35S

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLV-SFFV-ZFYVE27 S14D S25D S32D T261D-WPRE-UbC-mCherry

    Plasmid

    #214471

    Purpose

    Lentiviral vector plasmid expressing human ZFYVE27 mutations S14D S25D S32D T261D under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Inserts

    ZFYVE27 (ZFYVE27 Human)
    mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    S14D S25D S32D T261D

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV-SFFV-Llgl1-WPRE-UbC-Emerald

    Plasmid

    #203805

    Purpose

    Lentiviral vector plasmid expressing mouse Llgl1 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Inserts

    Llgl1 (Llgl1 Mouse)
    Emerald
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDONR221-W279A-short-UBASH3A-Ctag

    Plasmid

    #195402

    Purpose

    Human W279A short-form UBASH3A in Gateway pDONR221 (for C-terminal tag)

    Depositor

    Patrick Concannon

    Article

    Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

    Insert

    UBASH3A (UBASH3A Human)
    

    Use

    Gateway donor vector

    Mutation

    W279A

    Available Since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR221-K202R-short-UBASH3A-Ctag

    Plasmid

    #195399

    Purpose

    Human K202R short-form UBASH3A in Gateway pDONR221 (for C-terminal tag)

    Depositor

    Patrick Concannon

    Article

    Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

    Insert

    UBASH3A (UBASH3A Human)
    

    Use

    Gateway donor vector

    Mutation

    K202R

    Available Since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. K202R-short-UBASH3A-V5-6xHis

    Plasmid

    #195406

    Purpose

    Mammalian expression of human K202R short-form UBASH3A with V5-6xHis tag

    Depositor

    Patrick Concannon

    Article

    Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

    Insert

    UBASH3A (UBASH3A Human)
    

    Tags

    V5-6xHis

    Expression

    Mammalian

    Mutation

    K202R

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. W279A-short-UBASH3A-V5-6xHis

    Plasmid

    #195409

    Purpose

    Mammalian expression of human W279A short-form UBASH3A with V5-6xHis tag

    Depositor

    Patrick Concannon

    Article

    Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

    Insert

    UBASH3A (UBASH3A Human)
    

    Tags

    V5-6xHis

    Expression

    Mammalian

    Mutation

    W279A

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. HJP-178-IVS-Syn21-CreAB-NrdJ-1N-WPREw

    Plasmid

    #162473

    Purpose

    This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

    Depositor

    Benjamin White

    Article

    Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

    Insert

    CreAB
    

    Use

    Cre/Lox

    Available Since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. HJP-208-IVS-Syn21-CreAB-NrdJ-1N-WPREUw

    Plasmid

    #162478

    Purpose

    This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

    Depositor

    Benjamin White

    Article

    Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

    Insert

    CreAB
    

    Use

    Cre/Lox

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. HJP-195-IVS-Syn21-gp41-1C-CreB-NrdJ-1N-WPREUw

    Plasmid

    #162476

    Purpose

    This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

    Depositor

    Benjamin White

    Article

    Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

    Insert

    CreB
    

    Use

    Cre/Lox

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. HJP-194-IVS-Syn21-CreA-gp41-1N-WPREUw

    Plasmid

    #162475

    Purpose

    This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

    Depositor

    Benjamin White

    Article

    Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

    Insert

    CreA
    

    Use

    Cre/Lox

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. HJP-179-IVS-Syn21-NrdJ-1C-CreC-WPREw

    Plasmid

    #162472

    Purpose

    This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

    Depositor

    Benjamin White

    Article

    Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

    Insert

    CreC
    

    Use

    Cre/Lox

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. HJP-176-IVS-Syn21-CreA-gp41-1N-WPREw

    Plasmid

    #162470

    Purpose

    This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

    Depositor

    Benjamin White

    Article

    Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

    Insert

    CreA
    

    Use

    Cre/Lox

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. HJP-196-IVS-Syn21-NrdJ-1C-CreC-WPREUw

    Plasmid

    #162477

    Purpose

    This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

    Depositor

    Benjamin White

    Article

    Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

    Insert

    CreC
    

    Use

    Cre/Lox

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. HJP-180-IVS-Syn21-gp41-1C-CreB-NrdJ-1N-WPREw

    Plasmid

    #162471

    Purpose

    This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

    Depositor

    Benjamin White

    Article

    Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

    Insert

    CreB
    

    Use

    Cre/Lox

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. HJP-207-IVS-Syn21-gp41-1C-CreBC-WPREUw

    Plasmid

    #162479

    Purpose

    This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

    Depositor

    Benjamin White

    Article

    Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

    Insert

    CreBC
    

    Use

    Cre/Lox

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. HJP-177-IVS-Syn21-gp41-1C-CreBC-WPREw

    Plasmid

    #162474

    Purpose

    This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

    Depositor

    Benjamin White

    Article

    Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

    Insert

    CreBC
    

    Use

    Cre/Lox

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. HJP-473-DNE>FLP-T2A-CreAB-NrdJ-1N

    Plasmid

    #162480

    Purpose

    This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

    Depositor

    Benjamin White

    Article

    Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

    Insert

    DNE-Frterminator
    

    Use

    Cre/Lox

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBITE-CD19-CD3

    Plasmid

    #190677

    Purpose

    Encodes a modularized form of HD37-OKT3 bispecific antibody

    Depositor

    Scott McComb

    Article

    Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

    Insert

    Modularized-HD37-modularized-OKT3-P2A-NeonGreen
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pUC57-mitoTPNOX

    Plasmid

    #87854

    Purpose

    This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.

    Depositor

    Vamsi Mootha

    Article

    Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/

    Insert

    mitoTPNOX
    

    Tags

    C-terminal FLAG tag with a linker and mitochondri…

    Expression

    Bacterial

    Available Since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3.1-H2-18 Heavy chain-SpyTag003

    Plasmid

    #216307

    Purpose

    Expresses anti-HER2 H2-18 Heavy chain-SpyTag003 in mammalian cells. To be paired with H2-18 light chain to form the anti-HER2 H2-18 Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    H2-18 Heavy chain-SpyTag003
    

    Use

    Affinity Reagent/ Antibody

    Tags

    His6 tag, Signal peptide, and SpyTag003

    Expression

    Mammalian

    Promoter

    T7 promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET28a-SnoopCatcher

    Plasmid

    #72322

    Purpose

    Bacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.

    Depositor

    Mark Howarth

    Article

    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

    Insert

    pET28a-SnoopCatcher
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    changed G842T and D848G of S. pneumoniae adhesin …

    Promoter

    T7

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET15bpATnp

    Plasmid

    #121137

    Purpose

    This vector encode a His6-tagged proteinA-Tnp(hyperactive form) fusion protein.

    Depositor

    Keji Zhao

    Article

    Carter et al Nat Commun. 2019 Aug 20;10(1):3747. doi:

    Insert

    Protein A-Tnp
    

    Tags

    his

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3.1 MCS-BirA(R118G)-HA

    Plasmid

    #36047

    Purpose

    To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tag

    Depositor

    Kyle Roux

    Article

    Roux et al J Cell Biol. 2012 Mar 12.

    Has Service

    Cloning Grade DNA

    Insert

    BirA(R118G)-HA
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    R118G (highly promiscuous form)

    Available Since

    April 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET28a SnoopTag-MBP

    Plasmid

    #72323

    Purpose

    Bacterial expression of Maltose Binding Protein linked to SnoopTag, a peptide tag forming a spontaneous covalent bond with its protein partner Snoopcatcher.

    Depositor

    Mark Howarth

    Article

    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

    Insert

    pET28a SnoopTag-MBP
    

    Tags

    N-terminal His6 tag. MBP is fused at the C-termin…

    Expression

    Bacterial

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. scFLARE1

    Plasmid

    #158738

    Purpose

    Mammalian expression of scFLARE1 component

    Depositor

    Alice Ting

    Article

    Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

    Insert

    scFLARE1
    

    Use

    AAV

    Tags

    Flag, V5

    Expression

    Mammalian

    Mutation

    Truncated form of TEV protease (220-242 amino aci…

    Promoter

    CMV

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCX-mutSpiCee

    Plasmid

    #140903

    Purpose

    mammalian expression of a mutated form of SpiCee, that does not affect calcium signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

    Insert

    mutSpiCee
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pHR_Gal4UAS_human_leucinezipperTRAIL _IRES_mCherry_PGK_tBFP

    Plasmid

    #85433

    Purpose

    Lentiviral vector for Gal4 inducible leucinezipper TRAIL with mCherry reporter and constitutive tBFP expression

    Depositor

    Wendell Lim

    Article

    Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

    Insert

    leucine zipper human TRAIL secreted form
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

44. MBP-TEVcs(ENLYFQ/G)-His6-uTEV2Δ(220-242)-R5

    Plasmid

    #135456

    Purpose

    Bacterial expression of uTEV2Δ protease

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5
    

    Tags

    MBP, His6

    Expression

    Bacterial

    Mutation

    S219V mutation improves stability, T30A/S153N mut…

    Promoter

    tac promoter

    Available Since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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45. pMSCVhyg-3xT7-nSREBP1a

    Plasmid

    #171030

    Purpose

    Expresses 3xT7-tagged nuclear form of SREBP1a in mammalian cells

    Depositor

    Kai Ge

    Article

    Jang et al Genes Dev. 2021 Apr 22. pii: gad.347583.

    Insert

    nSREBP1a (Srebf1 Mouse)
    

    Use

    Retroviral

    Tags

    3xT7

    Expression

    Mammalian

    Mutation

    amino acids 1- 449

    Promoter

    5' LTR

    Available Since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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46. MBP-TEVcs(ENLYFQ/G)-His6-uTEV1Δ(220-242)-R5

    Plasmid

    #135455

    Purpose

    Bacterial expression of uTEV1Δ protease

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    MBP-TEVcs(ENLYFQ/G)-His6-TEV1Δ(220-242)-R5
    

    Tags

    MBP, His6

    Expression

    Bacterial

    Mutation

    S219V mutation improves stability, S153N mutation…

    Promoter

    tac promoter

    Available Since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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47. pcDNA3-myc NES-mCE NLS

    Plasmid

    #82466

    Purpose

    Expresses cytoplasmic restricted form of myc tagged mRNA capping enzyme

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    NES mRNA capping enzyme (Rngtt HIV, Mouse)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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48. GFP-Rab7Q67L

    Plasmid

    #169038

    Purpose

    Express human constitutively active form of Rab7a(Q67L) in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

    Insert

    Rab7a (RAB7A Human)
    

    Tags

    acGFP1

    Expression

    Mammalian

    Mutation

    Q67L

    Promoter

    CMV

    Available Since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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49. pcDNA-Flag-Kdm6b(1025-End)-pA

    Plasmid

    #100278

    Purpose

    In vitro transcription template (T7 promoter) for Kdm6b catalytic domain active form.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nature. 2017 Jul 19. doi: 10.1038/nature

    Insert

    Kdm6b (Kdm6b Mouse)
    

    Tags

    Flag epitope tag

    Expression

    Mammalian

    Mutation

    catalytic domain (1025aa-End)

    Promoter

    T7

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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50. pMT-Hrp48-ADARcd-E488Q-V5

    Plasmid

    #139685

    Purpose

    Expresses the hyperactive form of the catalytic domain of the ADAR protein fused to HRP-48 for use in "TRIBE" assays

    Depositor

    Michael Rosbash

    Article

    Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126

    Insert

    Hrp48, ADAR (Adar Fly)
    

    Tags

    V5

    Expression

    Bacterial and Insect

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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