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pTRE3G-human cGAS-HA

Plasmid

#186842

Purpose

Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Human cGAS with C-terminal HA tag (Adamts1 Human)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

TRE3G

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pHis-3xFLAG-CMV14-CYB5R3

Plasmid

#204551

Purpose

Expresses 3xFLAG-tagged CYB5R3 in mammalian cells

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

CYB5R3 (CYB5R3 Human)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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YA1627: pAAV_hSyn-Dio-paQuasAr3-s-P2A-CheRiff-s

Plasmid

#107704

Purpose

in vivo voltage imaging

Depositor

Adam Cohen

Article

Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

Insert

paQuasAr3-P2A-CheRiff

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N3-CYB5R3

Plasmid

#204567

Purpose

Expresses EGFP-tagged CYB5R3 in mammalian cells

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

CYB5R3 (CYB5R3 Human)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLEX307-mFzd6

Plasmid

#102868

Purpose

Expresses mouse Fzd6 in mammalian cells

Depositor

Henry Ho

Article

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Insert

Frizzled 6 (Fzd6 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pLEX307-mFzd1

Plasmid

#102864

Purpose

Expresses mouse Fzd1 in mammalian cells

Depositor

Henry Ho

Article

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Insert

Frizzled 1 (Fzd1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pLEX307-mFzd3

Plasmid

#102865

Purpose

Expresses mouse Fzd3 in mammalian cells

Depositor

Henry Ho

Article

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Insert

Frizzled 3 (Fzd3 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE3G-human cGAS-GFP

Plasmid

#186844

Purpose

Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Human cGAS with C-terminal GFP tag (Adamts1 Human)

Use

Retroviral

Tags

GFP

Expression

Mutation

Promoter

TRE3G

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTRE3G-FLAG-human cGAS-HA

Plasmid

#186849

Purpose

Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Human cGAS with N-terminal FLAG and C-terminal HA tag (Adamts1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mutation

Promoter

TRE3G

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTRE3G-HA-human cGAS

Plasmid

#186851

Purpose

Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Human cGAS with N-terminal HA tag (Adamts1 Human)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

TRE3G

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pTRE3G-human cGAS

Plasmid

#186853

Purpose

Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Human cGAS without tags (Adamts1 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

TRE3G

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX6p-1-UFBP1 M5

Plasmid

#204563

Purpose

Expresses GST-tagged UFBP1 M5 in E.coli

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

UFBP1 (DDRGK1 Human)

Use

Tags

Expression

Bacterial

Mutation

215-314aa

Promoter

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N3-CYB5R3 deltaN26

Plasmid

#204568

Purpose

Expresses EGFP-tagged CYB5R3 deltaN26 in mammalian cells

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

CYB5R3 (CYB5R3 Human)

Use

Tags

Expression

Mammalian

Mutation

delta2-26aa

Promoter

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX6p-1-UFBP1 M3

Plasmid

#204561

Purpose

Expresses GST-tagged UFBP1 M3 in E.coli

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

UFBP1 (DDRGK1 Human)

Use

Tags

Expression

Bacterial

Mutation

116-314aa

Promoter

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX6p-1-UFBP1 M4

Plasmid

#204562

Purpose

Expresses GST-tagged UFBP1 M4 in E.coli

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

UFBP1 (DDRGK1 Human)

Use

Tags

Expression

Bacterial

Mutation

116-214aa

Promoter

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX6p-1-UFBP1 F196A V198A

Plasmid

#204564

Purpose

Expresses GST-tagged UFBP1 F196A V198A in E.coli

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

UFBP1 (DDRGK1 Human)

Use

Tags

Expression

Bacterial

Mutation

F196A V198A

Promoter

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pmCherry-EGFP-N3-CYB5R3 K214R

Plasmid

#204570

Purpose

Expresses mCherry-EGFP-tagged CYB5R3 K214R in mammalian cells

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

CYB5R3 (CYB5R3 Human)

Use

Tags

Expression

Mammalian

Mutation

K214R

Promoter

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pmCherry-EGFP-N3-CYB5R3

Plasmid

#204569

Purpose

Expresses mCherry-EGFP-tagged CYB5R3 in mammalian cells

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

CYB5R3 (CYB5R3 Human)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAsh-MCL-GABARAP

Plasmid

#204574

Purpose

Expresses Ash-tagged GABARAP in mammalian cells

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

GABARAP (GABARAP Human)

Use

Tags

Expression

Mammalian

Mutation

deltaL117

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG1.1-Armc12 delta ARM/FLAG

Plasmid

#180496

Purpose

Expression vector of ARM domain deleted mouse Armc12 tagged with FLAG at C-terminus. See Fig.4D of Shimada, K. et al. Proc. Natl. Acad. Sci. USA. 118 (6): e2018355118, 2021.

Depositor

Masahito Ikawa

Article

Shimada et al Proc Natl Acad Sci U S A. 2021 Feb 9;118

Insert

armadillo repeat containing 12 (Armc12 Mouse)

Use

Tags

FLAG tag

Expression

Mammalian

Mutation

ARM domain of Armc12 is deleted

Promoter

CAG promoter

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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