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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,467 results for: PUC19

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Showing: 601 - 620 of 2467 results

  3. AICSDP-28:TUBA1B-mTagRFP-T

    Plasmid
    #101785
    Purpose
    Homology arms and mTagRFPT-linker sequence for N-terminus (second exon) tagging of human TUBA1B
    Depositor
    Allen Institute for Cell Science
    Insert
    TUBA1B Homology Arms with mTagRFPT-linker (TUBA1B Human)
    Use
    CRISPR; Donor template
    Tags
    mTagRFPT-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    Oct. 31, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101785-plasmid-map-sequence-id-197676

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  4. pSCW01

    Plasmid
    #72300
    Purpose
    Starting substrate for in vitro DNA mismatch repair substrate monitored by PstI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.
    Depositor
    Peggy Hsieh
    Article
    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.
    Type
    Empty backbone
    Use
    Derivative of puc19
    Expression
    Bacterial
    Promoter
    lac
    Available Since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    72300-plasmid-map-sequence-id-255196

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  9. pADH110

    Plasmid
    #90982
    Purpose
    NAT-marked pSNR52 promoter fragment for use in gRNA expression cassette stitching PCR; for use with pADH119, 139, or 147 to generate target-specific gRNA expression cassette.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Inserts
    NAT 2 of 2
    pSNR52
    Use
    CRISPR
    Available Since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90982-plasmid-map-sequence-id-180510

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  17. pTO149_RFP_CauNEO

    Plasmid
    #177277
    Purpose
    Encodes a G418 (NEOr) selectable marker with codon-optimization for expression in CUG clade fungi. Also encodes RFP tagging of Candida auris ENO1 to measure transformation efficiency.
    Depositor
    Teresa O'Meara
    Article
    Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:
    Insert
    RFP, CauNEO
    Tags
    ENO1-RFP
    Expression
    Bacterial
    Mutation
    All CUG Leucine codons were replaced with the syn…
    Promoter
    AgTEF2
    Available Since
    Nov. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    177277-plasmid-map-sequence-id-347241

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  18. pUA97

    Plasmid
    #69340
    Purpose
    It encodes a fluorescent protein that can be fused to a gene of interest using SEC CRISPR strategy
    Depositor
    Oliver Hobert
    Insert
    ceBFP SEC 3xFlag
    Tags
    3xFlag and ceBFP (mTagBFP)
    Expression
    Worm
    Mutation
    sqt-1(e1350)
    Available Since
    Dec. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    69340-plasmid-map-sequence-id-133241

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Showing: 601 - 620 of 2467 results