We narrowed to 3,245 results for: Streptococcus

Showing: 601 - 620 of 3245 results

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pRRL-gRNA(anti-cGAS)-Cas9-Puro

Plasmid

#130908

Purpose

gRNA targeting human cGAS; Cas9 insert; Puromycin selection marker

Depositor

Jonathan Kagan

Publication

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9

Expression

Mammalian

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pET28b-NmCas9-Flag

Plasmid

#109030

Purpose

Wild type Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

Depositor

Yan Zhang

Publication

Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

Insert

CRISPR-associated endonuclease Cas9

Tags

Flag Tag

Expression

Bacterial

Promoter

T7

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-1BPNLS-Cas9-1BPNLS

Plasmid

#87108

Purpose

BPNLS-Cas9-BPNLS expression plasmid

Depositor

Juan Belmonte

Publication

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

1BPNLS-Cas9-1BPNLS

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CAG

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pJSC090 - Bacterial expression plasmid for SpCas9 + K855A variant

Plasmid

#101213

Purpose

Bacterial expression plasmid for SpCas9 + K855A variant

Depositor

Jennifer Doudna, Keith Joung

Publication

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant K855A

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

K855A

Promoter

T7

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA™5/FRT/CMV_promoter-Cas9-E2A-mRFP

Plasmid

#164131

Purpose

Expresses SpCas9 and mRFP in mammalian cells (an insertion vector to generate Cas9-knockin cell lines)

Depositor

Hyongbum Kim

Publication

Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SpCas9 (cas9 Synthetic)

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pX330A_D10A-1x4

Plasmid

#58774

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Publication

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV_CK8e_Cas9_miniPA

Plasmid

#226141

Purpose

AAV construct for HITI insert with CK8e promoter and SpCas9

Depositor

Christopher Nelson

Publication

Padmaswari et al Mol Ther Nucleic Acids. 2024 Sep 2;35(4)

Insert

Cas9

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CK8e

Available since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-MP-84-spCas9-U6-gRNA

Plasmid

#206935

Purpose

Expression of spCas9 endonuclease and gRNA in an AAV vector allowing packaging of viral genome smaller than 5 kb.

Depositor

Markus Grompe

Insert

spCas9

Use

AAV

Tags

SV40 NLS

Expression

Mammalian

Promoter

MP-84

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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YN2_1_LT58_X2site

Plasmid

#177705

Purpose

Expresses Cas9 and sgRNA cassette for targeting site X2 in yeast Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Publication

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Insert

Cas9

Use

CRISPR

Expression

Yeast

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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px330 EZH2 - pDY052

Plasmid

#171107

Purpose

Expresses Cas9 and an sgRNA targeting EZH2

Depositor

Thomas Cech

Publication

Youmans et al Genes Dev. 2018 Jun 1;32(11-12):794-805.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9

Expression

Mammalian

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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GluN2A (px330:2-guideCas9)

Plasmid

#169428

Purpose

Expression of Cas9 and 2 guides

Depositor

Richard Huganir

Publication

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

spCas9

Use

CRISPR

Expression

Mammalian

Available since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pRDA_085

Plasmid

#158583

Purpose

Lentiviral expression of SpCas9; P2A site provides mKate2

Depositor

John Doench, David Root

Publication

Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

Insert

SpCas9

Use

Lentiviral

Expression

Mammalian

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDB4279

Plasmid

#98698

Purpose

A control plasmid containing the intact bsdMX marker

Depositor

Li-Lin Du

Publication

Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

Insert

Cas9

Use

CRISPR

Expression

Yeast

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pSHS212

Plasmid

#87346

Purpose

Cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid

Depositor

Jennifer Doudna

Publication

Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

Insert

Cas9

Tags

His-MBP

Expression

Bacterial

Mutation

C80S/C574S

Promoter

T7

Available since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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pICH47742:FCP:Cas9YFP

Plasmid

#85986

Purpose

Golden Gate Level 1 cassette encoding a Cas9-YFP fusion under the FCP promoter

Depositor

Thomas Mock

Publication

Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cas9

Use

CRISPR; Golden gate assembly

Tags

SV40 NLS, YFP

Promoter

FCP

Available since

Jan. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pBLO1806_Cas9_2xNLS_human

Plasmid

#74493

Purpose

Human codon optimized plasmid to express Cas9 t2a mCherry w/2xNLS and a sgRNA

Depositor

David Savage

Publication

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

Cas9

Tags

t2a mCherry

Expression

Mammalian

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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Lenti_PGK-Cas9-P2A-HygR

Plasmid

#164135

Purpose

Lentiviral construct for the expression of SpCas9 driven by PGK promoter in mammalian cells

Depositor

Hyongbum Kim

Publication

Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

Insert

SpCas9 (cas9 Synthetic)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

PGK

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Lenti_Efs_hSpCas9_NLS_FLAG-WPRE

Plasmid

#97313

Purpose

Lentiviral vector for encoding a human codon-optimized SpCas9 driven by EFs promoter.

Depositor

Hui Yang

Publication

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

humanized S. pyogenes Cas9

Use

Lentiviral and Mouse Targeting

Tags

Flag

Expression

Mammalian

Promoter

EFS

Available since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV_LP1B_St1Cas9_LMD-9_SpA_U6_sgRNA

Plasmid

#110624

Purpose

A single vector AAV-Cas9 system containing a liver-specific promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA

Depositor

Yannick Doyon

Publication

Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

Inserts

St1Cas9 LMD-9
sgRNA for St1Cas9

Use

AAV, CRISPR, and Mouse Targeting

Tags

SV40 NLS

Expression

Mammalian

Promoter

LP1B and hU6

Available since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-SpCas9

Plasmid

#242844

Purpose

Entry vector for Cas9 gateway cloning into a PiggyBac plasmid

Depositor

Siyuan Zhang

Publication

Aleksandrovic et al Nat Commun. 2026 Sep 3;17(1):10497. doi:

Insert

Cas9 (cas9 S. pyogenes)

Use

CRISPR, Gateway Cloning, and Synthetic Biology

Tags

Flag

Expression

Bacterial

Available since

Oct. 7, 2026

Availability

Academic Institutions and Nonprofits only

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