We narrowed to 23,942 results for: nes

1. pLentiRNACRISPR_006 - hU6-DR_BsmBI-EFS-RfxCas13d-NES-2A-Puro-WPRE

   Plasmid

   #138148

   Purpose

   Expresses RfxCas13d in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   CasRx
   

   Use

   Lentiviral

   Tags

   NES

   Expression

   Mutation

   Promoter

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLV hU6-sgRNA Nestin-dCas9-KRAB-T2a-GFP

   Plasmid

   #196988

   Purpose

   Derived from pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP, custom sgRNA cloning vector

   Depositor

   Elizabeth Kirby

   Article

   Dause et al Mol Neurobiol. 2025 Mar 25. doi: 10.1007

   Insert

   Nestin-dCas9-KRAB-T2a-GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLentiRNACRISPR_004 - hU6-DR_BsmBI-EFS-PspCas13b-NES-2A-Puro-WPRE

   Plasmid

   #138146

   Purpose

   Expresses PspCas13b in mammalian cells, localized in the cytoplasm. For cloning guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang acac. R overhang caac

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   PspCas13b
   

   Use

   Lentiviral

   Tags

   NES

   Expression

   Mutation

   Promoter

   Available since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLV-miRFP680-NES-p2A-ezrin-mRuby3-IRES-Neo

   Plasmid

   #222636

   Purpose

   Ezrin activation reporter

   Depositor

   Arnold Hayer

   Article

   Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

   Insert

   miRFP680-NES-p2A-ezrin-mRuby3 (EZR Human)
   

   Use

   Lentiviral

   Tags

   mRuby3

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV-TM-KA2-CaM-NES-TevN-AsLOV2-TEVseq-tTA

   Plasmid

   #171615

   Purpose

   Soma-targeted Cal-Light with KA2

   Depositor

   Hyungbae Kwon

   Article

   Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

   Insert

   TM-KA2-CaM-NES-TevN-AsLOV2-TEVseq-tTA
   

   Use

   Synthetic Biology

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-HA-β2AR-NNES-NanoLuc-15aa linker-AsLOV2(V416L)

   Plasmid

   #125224

   Purpose

   Expresses β2AR-NanoLuc-AsLOV2(V416L) in mammalian cells

   Depositor

   Alice Ting

   Article

   Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

   Insert

   HA-β2AR-NNES-NanoLuc-15aa linker-AsLOV2(V416L)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CMV:CD4-HA-CIBN-MKII-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4

   Plasmid

   #135460

   Purpose

   Mammalian expression of uFLARE TF component

   Depositor

   Alice Ting

   Article

   Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

   Insert

   CMV-CD4-HA-CIBN-MKII-NNES-hLOV-TEVcs( ENLYFQM)-GAL4.ape
   

   Use

   Tags

   HA, flag,

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLentiRNACRISPR_002 - hU6-DR_BsmBI-EFS-PguCas13b-NES-2A-Puro-WPRE

   Plasmid

   #138144

   Purpose

   Expresses PguCas13b in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   PguCas13b
   

   Use

   Lentiviral

   Tags

   NES

   Expression

   Mutation

   Promoter

   Available since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-β2AR-NNES-NanoLuc-eLOV-TEVcs-Flag-Gal4-V5

   Plasmid

   #125236

   Purpose

   Expresses modified SPARK β2AR-NanoLuc transmembrane component in mammalian cells

   Depositor

   Alice Ting

   Article

   Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

   Insert

   β2AR-NNES-NanoLuc-eLOV-TEVcs-Flag-Gal4-V5
   

   Use

   AAV

   Tags

   Flag, V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry

    Plasmid

    #210504

    Purpose

    expresses NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry component in mammalian cells

    Depositor

    Dongmin Lee

    Article

    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

    Insert

    NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-TM-Kv2.1-CaM-NES-TevN-AsLOV2-TEVseq-tTA

    Plasmid

    #171616

    Purpose

    Soma-targeted Cal-Light with Kv2.1

    Depositor

    Hyungbae Kwon

    Article

    Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

    Insert

    TM-Kv2.1-CaM-NES-TevN-AsLOV2-TEVseq-tTA
    

    Use

    Synthetic Biology

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-IgK sp-HA-CD4-10aa linker-CIBN-NNES-NanoLuc

    Plasmid

    #125229

    Purpose

    Expresses transmembrane intracellular NanoLuc in mammalian cells

    Depositor

    Alice Ting

    Article

    Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

    Insert

    IgK sp-HA-CD4-10aa linker-CIBN-NNES-NanoLuc
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pzk490-pmax-NES-dpspCas13b(AAAA)-FseI[ADAR2-DD-splitC]

    Plasmid

    #196843

    Purpose

    Express NES-dpspCas13b(AAAA)-FseI[ADAR2-DD-splitC]

    Depositor

    Albert Cheng

    Article

    Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

    Insert

    NES-dpspCas13b(AAAA)-FseI[ADAR2-DD-splitC]
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AAV-Synapsin-NLS-mTurquoise2dC11-408LOV2-Ja(delta)-NES21-WPRE

    Plasmid

    #159961

    Purpose

    optoNES construct with modified mTurquoise2-LOV2 RET

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    NLS-mTurquoise2dC11-408LOV2-Ja(delta)-NES21
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAV-CMV-NLS-mTurquoise2dC11-408LOV2-Ja(delta)-NES21-WPRE

    Plasmid

    #159965

    Purpose

    optoNES construct with modified mTurquoise2-LOV2 RET

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    NLS-mTurquoise2dC11-408LOV2-Ja(delta)-NES21
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    CMV-IE

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAV-CMV-NLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21-WPRE

    Plasmid

    #159964

    Purpose

    optoNES construct with modified mTurquoise2-LOV2 RET

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    NLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    CMV-IE

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAV-Synapsin-NLS-mTurquoise2dC8-408LOV2-Ja(delta)-NES21-WPRE

    Plasmid

    #159958

    Purpose

    optoNES construct with modified mTurquoise2-LOV2 RET

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    NLS-mTurquoise2dC8-408LOV2-Ja(delta)-NES21
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV-Synapsin-NLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21-WPRE

    Plasmid

    #159957

    Purpose

    optoNES construct with modified mTurquoise2-LOV2 RET

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    NLS-mTurquoise2dC7-408LOV2-Ja(delta)-NES21
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV-Synapsin-NLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21-WPRE

    Plasmid

    #159960

    Purpose

    optoNES construct with modified mTurquoise2-LOV2 RET

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    NLS-mTurquoise2dC10-408LOV2-Ja(delta)-NES21
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAV-Synapsin-NLS-mTurquoise2dC9-408LOV2-Ja(delta)-NES21-WPRE

    Plasmid

    #159959

    Purpose

    optoNES construct with modified mTurquoise2-LOV2 RET

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    NLS-mTurquoise2dC9-408LOV2-Ja(delta)-NES21
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge