We narrowed to 24,787 results for: nes

1. pSIN-hSNCA-NE

   Plasmid

   #102366

   Purpose

   Lentiviral overexpression of synuclein alpha

   Depositor

   Shu Leong Ho

   Article

   Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

   Insert

   synuclein alpha (SNCA Human)
   

   Use

   Lentiviral

   Tags

   NE

   Expression

   Mammalian

   Mutation

   A silent mutation (333bp downstream of ATG) was i…

   Promoter

   EF-1a

   Available Since

   April 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-hUCP4-NE

   Plasmid

   #102362

   Purpose

   Overexpression of solute carrier family 25 member 27 in mammalian cells

   Depositor

   Shu Leong Ho

   Insert

   solute carrier family 25 member 27 (SLC25A27 Human)
   

   Tags

   NE

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. FUW-hSNCA-NE

   Plasmid

   #215486

   Purpose

   Lentiviral expression of human alpha-synuclein in mammalian cells

   Depositor

   Ophir Shalem

   Article

   Santhosh Kumar et al Sci Adv. 2024 Feb 9;10(6):eadj4767. doi:

   Insert

   alpha-synuclein (SNCA Human)
   

   Use

   Lentiviral

   Tags

   NE tag

   Expression

   Mammalian

   Promoter

   Ubc

   Available Since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGFP-nE-mHP1alpha

   Plasmid

   #181895

   Purpose

   Fluorescently tagged HP1alpha, serines in N-terminal extension (NTE) replaced by glutamates

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   nE-mHP1alpha (Cbx5 Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   S11E, S12E, S13E, S14E

   Promoter

   CMV

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A

   Plasmid

   #103865

   Purpose

   Catalytically inactive PSPCas13b. RNase activity is inactivated by mutation of catalytic histidine residues in the HEPN domains (H133A/H1058A).

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   PspCas13b
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and HIV NES

   Expression

   Mammalian

   Mutation

   Contains the H133A and H1058A mutations which ina…

   Promoter

   EF1a

   Available Since

   Jan. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

   Plasmid

   #92392

   Purpose

   produces AAV expressing Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

   Insert

   TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
   

   Use

   AAV and Synthetic Biology

   Expression

   Mammalian

   Promoter

   Human Synapsin

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDV06_feedback_entry_TagBFP-2A-MCS-2A-mNeonGreen-2A-MCP-ADAR2dd(E488Q)-NES

   Plasmid

   #200402

   Purpose

   Entry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses mNeonGreen and MCP-ADAR upon target detection

   Depositor

   James Collins

   Article

   Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

   Insert

   Closed-loop DART VADAR sensor entry cassette
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAG385 bJun-NFAST-NLS-DEVDG-mCherry-NES

   Plasmid

   #130816

   Purpose

   Expresses bJun-NFAST-NLS-DEVDG-mCherry-NES in mammalian cells

   Depositor

   Arnaud Gautier

   Article

   Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

   Insert

   bJun-NFAST-NLS-DEVDG-mCherry-NES
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV_hsyn_NES-his-CaMPARI2-F391W-G395D-WPRE-SV40

   Plasmid

   #101063

   Purpose

   AAV vector expressing CaMPARI2_F391W-G395D (Kd = 530nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

   Depositor

   Eric Schreiter

   Article

   Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

   Insert

   CaMPARI2_F391W-G395D
   

   Use

   AAV

   Tags

   FLAG-HA-myc and NES_his

   Promoter

   hsyn (synapsin-1)

   Available Since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDV21_feedback_entry_TagBFP-2A-MCS-2A-NLuc-2A-MCP-ADAR2dd(E488Q)-NES

    Plasmid

    #200403

    Purpose

    Entry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses NanoLuc and MCP-ADAR upon target detection

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    Closed-loop DART VADAR sensor entry cassette
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

    Plasmid

    #92390

    Purpose

    expresses Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA component in mammalian cells

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

    Insert

    TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax

    Plasmid

    #173872

    Purpose

    Mammalian expression of mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax (opto-Cdc42)

    Depositor

    Takao Nakata

    Article

    Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

    Insert

    mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax
    

    Tags

    C-terminal CAAX-box and mCherry

    Expression

    Mammalian

    Available Since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax

    Plasmid

    #173871

    Purpose

    Mammalian expression of mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax (opto-Rac1)

    Depositor

    Takao Nakata

    Article

    Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

    Insert

    mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax
    

    Tags

    C-terminal CAAX-box and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. EF1a-Cas9-2XNES-2ERT2-GCN4-SV40-Zeo

    Plasmid

    #120555

    Purpose

    Express Cas9, 2xNES, 2 tandem ERT2 and GCN4 in mammalian cells

    Depositor

    Yu Wang

    Article

    Lu et al Nucleic Acids Res. 2018 Mar 16;46(5):e25

    Insert

    Cas9, NES, ERT2 and GCN4
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVEGFR2_TEV(N)_NES_TCS(Q'L)_HA-dCas9(N)_P2A_Puro-WPRE

    Plasmid

    #101104

    Purpose

    Encodes dCas9(N) fused to VEGFR2

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    VEGFR2-dCas9(N)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5/FRT/TO/FLAG-HA-SBP-TNRC6A-NES-mut

    Plasmid

    #42046

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Tags

    FLAG, HA, and SBP

    Expression

    Mammalian

    Mutation

    F951A, F955A, I958A, F960A

    Available Since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-gLuc sp-NanoLuc-HA-β2AR-NNES-AsLOV2(V416L)

    Plasmid

    #125225

    Purpose

    Expresses NanoLuc-β2AR-AsLOV2(V416L) in mammalian cells

    Depositor

    Alice Ting

    Article

    Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

    Insert

    gLuc sp-NanoLuc-HA-β2AR-NNES-AsLOV2(V416L)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pzk384-pmax-PUFc-FseI-RESCUE-SalI-NES

    Plasmid

    #196831

    Purpose

    Express PUFc-RESCUE-S

    Depositor

    Albert Cheng

    Article

    Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

    Insert

    PUFc-RESCUE
    

    Expression

    Mammalian

    Available Since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. mVenus-NES-SspB-RGL2-GEF-P2A-iLIDcaax

    Plasmid

    #173875

    Purpose

    Mammalian expression of mVenus-NES-SspB-RGL2-GEF-P2A-iLIDcaax (opto-Ral)

    Depositor

    Takao Nakata

    Article

    Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

    Insert

    mVenus-NES-SspB-RGL2GEF-P2A-iLIDcaax
    

    Tags

    C-terminal CAAX-box and mVenus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. mCherry-NES-SspB-RGL2-GEF-P2A-iLIDcaax

    Plasmid

    #173876

    Purpose

    Mammalian expression of mCherry-NES-SspB-RGL2-GEF-P2A-iLIDcaax (opto-Ral)

    Depositor

    Takao Nakata

    Article

    Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

    Insert

    mCherry-NES-SspB-RGL2GEF-P2A-iLIDcaax
    

    Tags

    C-terminal CAAX-box and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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