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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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162,309 results

Showing: 9271 - 9300 of 162309 results

  1. BPK764

    Plasmid
    #65767
    Purpose
    Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Mutation
    Promoter
    T7 (x2)
    Available since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65767-plasmid-map-sequence-id-471631

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  5. GABA BR2-flag/pRK5

    Plasmid
    #197323
    Purpose
    Plasmid expressing an antigen targeting the GABA(B)R2 metabotropic GABA receptor
    Depositor
    Stephen Moss
    Insert
    GABA B R2 (Gabbr2 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    197323-plasmid-map-sequence-id-392892

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  8. pDR123

    Plasmid
    #37149
    Depositor
    Dale Ramsden
    Insert
    XRCC5 (XRCC5 Human)
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    July 18, 2012
    Availability
    Academic Institutions and Nonprofits only

  9. eGFP-proα2(I)-L

    Plasmid
    #120160
    Purpose
    Expresses mouse Type I procollagen α2 chain (Col1a2) with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site
    Depositor
    Sergey Leikin
    Article
    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11
    Insert
    Type I procollagen α2 chain (Col1a2 Mouse)
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Col1a2 exons 2-3 replaced by fluorescent tag
    Promoter
    CMV
    Available since
    Jan. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    120160-plasmid-map-sequence-id-233870

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  20. mCherry-Rab7a-7

    Plasmid
    #55127
    Purpose
    Localization: Ras GTPase, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Rab7a (RAB7A Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    N117S in NM_004637.5
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55127-plasmid-map-sequence-id-311825

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  23. mRFP-Rab7

    Plasmid
    #14436
    Purpose
    Mammalian expression of Rab7 fused to mRFP. Used for localization to the late endosome, and other early endosomal vacuoles.
    Depositor
    Ari Helenius
    Article
    Vonderheit et al PLoS Biol. 2005 Jul . 3(7):e233.
    Insert
    Rab 7 (RAB7A Canis familialis)
    Use
    Tags
    mRFP
    Expression
    Mammalian
    Mutation
    3 silent mutations near the C-terminus
    Promoter
    Available since
    March 23, 2007
    Availability
    Academic Institutions and Nonprofits only
    14436-plasmid-map-sequence-id-229535

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Showing: 9271 - 9300 of 162309 results