We narrowed to 6,639 results for: itch

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pPKm-105

Plasmid

#104853

Purpose

pcDNA3 - pCMV - PhyB NT - GBD, expressing Phytochrome B (PhyB) with Gal Binding Domain (GBD), under CMV promoter

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

PhyB NT GBD

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCS2-CRY2

Plasmid

#140572

Purpose

For use in light-induced protein inactivation through the interaction with CIB1

Depositor

Heidi Hehnly

Article

Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi:

Insert

CRY2 (CRY2 Mustard Weed)

Expression

Mammalian

Promoter

CMV IE94

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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PHR-GFP-p65

Plasmid

#183929

Purpose

Optogenetic PHR domain coupled to GFP and p65 activation domain; binds to CIBN upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

PHR-GFP-p65 (CRY2 Mustard Weed, Synthetic)

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV gfaABC1D 2xLyn-ERex-Venus-bPAC(F198Y)

Plasmid

#171117

Purpose

Astrocyte expression of PACmn, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity

Depositor

Thomas Oertner

Article

Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

Insert

2xLyn-ERex-Venus-bPAC(F198Y)

Use

AAV

Tags

Venus

Expression

Mammalian

Promoter

gfaABC1D

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pHR-TAF15N-miRFP670-Cry2WT

Plasmid

#122440

Purpose

Expresses disordered protein TAF15(1-208) fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

TAF15 (TAF15 Human)

Use

Lentiviral

Tags

Cry2WT and miRFP670

Expression

Mammalian

Mutation

Contains only amino acids 1-208

Promoter

SFFV

Available Since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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pCIBN-TetR

Plasmid

#103813

Purpose

BLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-TetR (CIB1, tetR Mustard Weed, E. coli)

Expression

Mammalian

Mutation

TetR: A4G (M2V)

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-PHR-iSH-T2A-CIBNcaax

Plasmid

#173860

Purpose

Mammalian expression of mCherry-PHR-iSH-T2A-CIBNcaax (opto-PI3K)

Depositor

Takao Nakata

Article

Kakumoto et al PLoS One. 2013 Aug 7;8(8):e70861. doi: 1

Insert

mCherry-PHR-iSH-T2A-CIBNcaax

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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LIC-Z

Plasmid

#153543

Purpose

perform light induced optogenetic clustering of TCR ζ-Chain as described in the associated publication

Depositor

Katharina Gaus

Article

Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

Insert

human TCR ζ-Chain (CD247 Human)

Tags

mCherry

Expression

Mammalian

Mutation

NA

Promoter

CMV

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pROF141

Plasmid

#155329

Purpose

Binary vector for expression of the PULSE system under the control of PAtUbi10 promoter

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

LB_PAtUbi10-SRDX-NLS-EL222-Tnos_SF_PAtUbi10-E-PIF6-NLS-Tnos_PAtUbi10-PhyB-VP16-NLS-Tnos_RB

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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qTAG-C-mNeon-Blast-TUBB4B

Plasmid

#207786

Purpose

Donor template for mNeon-2A-Blast insertion into the C-terminus of the TUBB4B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBB4B Addgene #207785

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

TUBB4B Homology Arms flanking a mNeon-Blast Cassette (TUBB4B Human)

Use

CRISPR; Donor template

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-gLuc sp-GS linker-HiBit-Flag-oβ2AR-TS-NNES-eLOV-TEVcs-Flag-Gal4-V5

Plasmid

#125234

Purpose

Expresses SPARK oβ2AR transmembrane component in mammalian cells

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

gLuc sp-GS linker-HiBit-Flag-oβ2AR-TS-NNES-eLOV-TEVcs-Flag-Gal4-V5

Use

AAV

Tags

Flag, V5

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-6p-hDcp2 E147/8Q

Plasmid

#72217

Purpose

bacterial expression of GST-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)

Depositor

Megerditch Kiledjian

Article

Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

Insert

Dcp2 (DCP2 Human)

Tags

GST

Expression

Bacterial

Mutation

E147Q, E148Q and F404L (please see depositor'…

Promoter

tac

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
Lyn-iTrkA-mCh-CRY2

Plasmid

#106169

Purpose

mammalian expression of Lyn-tagged intracellular domain of TrkA (aa 450-799) fused to mCherry and CRY2PHR (aa 1-498)

Depositor

Bianxiao Cui

Article

Duan et al ACS Synth Biol. 2018 Jul 20;7(7):1685-16

Insert

Lyn iTrkA mCherry CRY2PHR (CRY2 Rat, Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pB80-iLID-mCherry-RAB11

Plasmid

#174623

Purpose

Optogenetic coupling to recycling endosomes (RAB11) via iLID

Depositor

Lukas Kapitein

Article

Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

iLID-mCherry-RAB11 (RAB11A Human, Synthetic)

Tags

iLID-mCherry

Expression

Mammalian

Mutation

mCherry: Met1Del; RAB11: Met1Del

Promoter

Chicken beta-actin

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-CRY2–mPKCε-CAT-HA

Plasmid

#190483

Purpose

Expresses a fluorescently-tagged, optogenetically activated PKC-epsilon. Used in tandem with CIBN-CAAX

Depositor

Diomedes Logothetis

Article

Gada et al J Biol Chem. 2022 May;298(5):101893. doi

Insert

mCherry-CRY2–mPKCε-CAT-HA (Prkce Mouse, Synthetic)

Tags

Fused to CRY2, HA, and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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qTAG-C-moxGFP-Puro-H2BC11-MMEJ

Plasmid

#207760

Purpose

MMEJ donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

H2BC11 Short Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Cry2 mCherry PGK-1 in pcDNA3.1

Plasmid

#64214

Purpose

mCherry fused between Cry2 and PGK-1 to study cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

Inserts

Cry2 PHR (CRY2 Mustard Weed)
PGK1 (PGK1 Human)

Tags

mcherry

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-TM-Kv2.1-CaM-NES-TevN-AsLOV2-TEVseq-tTA

Plasmid

#171616

Purpose

Soma-targeted Cal-Light with Kv2.1

Depositor

Hyungbae Kwon

Article

Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

Insert

TM-Kv2.1-CaM-NES-TevN-AsLOV2-TEVseq-tTA

Use

Synthetic Biology

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax

Plasmid

#173866

Purpose

Mammalian expression of mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax (opto-Cdc42)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax

Tags

C-terminal CAAX-box and mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCLASP_Crz1(19A)_CLASP_RGS2membrane

Plasmid

#133085

Purpose

Plasmid contains Crz1* with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-Crz1*-yeLANS). CLASP modulates nuclear localization of Crz1* in response to blue light.

Depositor

Hana El-Samad

Article

Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

Insert

Crz1*-CLASP

Expression

Bacterial and Yeast

Promoter

pADH1

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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