We narrowed to 11,748 results for: pcdna

1. pcDNA3.1(+)-DnaJB1(H33Q)-PKAcat-mCherry

   Plasmid

   #181854

   Purpose

   mCherry-tagged DnaJB1-PKA catalytic subunit fusion with H33Q DnaJB1 mutation to block Hsp70 binding; for mammalian expression.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Histidine 33 in DnaJB1 changed to glutamine.

   Promoter

   CMV

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-MCS-BirA(R118G)-HA-TARG1

   Plasmid

   #172583

   Purpose

   For transient expression of C-terminal BirA-tagged TARG1

   Depositor

   Karla Feijs

   Article

   Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

   Insert

   TARG1 (OARD1 Human)
   

   Use

   Tags

   BirA(R118G)-HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-MCS-BirA(R118G)-HA-MACROD2

   Plasmid

   #172582

   Purpose

   For transient expression of C-terminal BirA-tagged MACROD2

   Depositor

   Karla Feijs

   Article

   Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

   Insert

   MACROD2 (MACROD2 Human)
   

   Use

   Tags

   BirA(R118G)-HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-MS2-HA-HsNanos3-del4-20_X

   Plasmid

   #147945

   Purpose

   Mammalian Expression of HsNanos3-del4-20

   Depositor

   Elisa Izaurralde

   Article

   Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

   Insert

   HsNanos3-del4-20 (NANOS3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-MS2-HA-HsRck_296-472-10xmut_AC

   Plasmid

   #148420

   Purpose

   Mammalian Expression of HsRck_296-472-10xmut

   Depositor

   Elisa Izaurralde

   Article

   Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

   Insert

   HsRck_296-472-10xmut (DDX6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Q309A, H312A, T316A, R320A, Q322A, N324A, R335A, …

   Promoter

   Available since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-kappa-myc-dK-2xG4S-TMst

   Plasmid

   #145766

   Purpose

   Expresses myc-dL5(E52K, L91S) low affinity Fluorogen Activating Protein (FAP) on the extracellular surface anchored to the mammalian cell plasma membrane with the PDGFR transmembrane domain.

   Depositor

   Marcel Bruchez

   Article

   Carpenter et al ACS Chem Biol. 2020 Aug 17. doi: 10.1021

   Insert

   kappa-myc-dK-2xG4S-TMst
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   The dK FAP is dL5 with mutations at E50K, L89S, a…

   Promoter

   CMV

   Available since

   Jan. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-Y478C-WLS-HA-IRES-GFP

   Plasmid

   #178063

   Purpose

   Expression of WLS-HA protein (Y478C mutant)

   Depositor

   Joseph Gleeson

   Article

   Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

   Insert

   WLS (WLS Human)
   

   Use

   Tags

   HA and IRES-GFP

   Expression

   Mammalian

   Mutation

   Y478C

   Promoter

   Available since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Y392C-WLS-HA-IRES-GFP

   Plasmid

   #178062

   Purpose

   Expression of WLS-HA protein (Y392C mutant)

   Depositor

   Joseph Gleeson

   Article

   Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

   Insert

   WLS (WLS Human)
   

   Use

   Tags

   HA and IRES-GFP

   Expression

   Mammalian

   Mutation

   Y392C

   Promoter

   Available since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-I531T-WLS-HA-IRES-GFP

   Plasmid

   #178064

   Purpose

   Expression of WLS-HA protein (I531T mutant)

   Depositor

   Joseph Gleeson

   Article

   Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

   Insert

   WLS (WLS Human)
   

   Use

   Tags

   HA and IRES-GFP

   Expression

   Mammalian

   Mutation

   I531T

   Promoter

   Available since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA4 3xFLAG Halo G-Linker nsp1

    Plasmid

    #175426

    Purpose

    Expresses G-linker CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    G-linker nsp1
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    G-linker in place of the RNase destabilization do…

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA4TO-3XFLAG Halo R99A nsp1

    Plasmid

    #175422

    Purpose

    Expresses R99A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV2 R99A nsp1
    

    Use

    Tags

    3XFLAG Halo

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1 LIC 6A CARD8-FLAG F405G

    Plasmid

    #169956

    Purpose

    Mammalian expression of CARD8-FLAG with a site III mutation (F405G)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    F405G

    Promoter

    CMV

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-2xFLAG-2xSTREP-CCND1(P287S)

    Plasmid

    #172644

    Purpose

    Expresses 2xFLAG-2xSTREP-tagged cyclin D1(P287S) in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Pro287Ser

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-2xFLAG-2xSTREP-CCND1(T286I)

    Plasmid

    #172645

    Purpose

    Expresses 2xFLAG-2xSTREP-tagged cyclin D1(T286I) in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Thr286Ile

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-2xFLAG-2xSTREP-CCND1(P287L)

    Plasmid

    #172646

    Purpose

    Expresses 2xFLAG-2xSTREP-tagged cyclin D1(P287L) in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Pro287Leu

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3HA-AMOT p130∆PPxY1(L106P)

    Plasmid

    #166714

    Purpose

    expresses AMOT(L106P) in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    Angiomotin p130 L106P (AMOT Human)
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    changed leucine 106 to proline

    Promoter

    CMV promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3HA-AMOT p130∆PPxY1(A112K)

    Plasmid

    #166715

    Purpose

    expresses AMOT(A112K) in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    Angiomotin p130 A112K (AMOT Human)
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    changed alanine 112 to lysine

    Promoter

    CMV promoter

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3_shRNA resistant PKA-Cα(E12,14K)-mEGFP

    Plasmid

    #168491

    Purpose

    Mammalian expression of the E12,14K mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.

    Depositor

    Haining Zhong

    Article

    Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

    Insert

    the E12,14K mutant of the mouse PKA catalytic subunit α C-terminally tagged by mEGFP. Resistant to shRNA knock-down.
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 LIC 6A CARD8-FLAG R394E

    Plasmid

    #169954

    Purpose

    Mammalian expression of CARD8-FLAG with a site III mutation (R394E)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    R394E

    Promoter

    CMV

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG

    Plasmid

    #169958

    Purpose

    Mammalian expression of the CARD8 C-terminus fused to ubiquitin (co-translationally processed)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG and Ub

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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