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Plasmid#203798PurposeLentiviral vector expressing human PIK3CA H1047R under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralTagsExpressionMammalianMutationH1047RPromoterAvailable sinceJuly 21, 2023AvailabilityAcademic Institutions and Nonprofits only
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pcDNA5-FRT/TO-FLAG-SHLD3
Plasmid#114123PurposeExpresses N-terminally tagged FLAG-SHLD3 in mammalian cellsDepositorInsertSHLD3 (SHLD3 Human)
UseGenomic integration, tetracycline inducibleTagsFLAGExpressionMammalianMutationPromoterCMV/TetO2Available sinceAug. 16, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYX-N-Puro-mNeon-TSC22D4
Plasmid#222671Purposeendogenously tag TSC22D4 with N-terminal mNeonDepositorInsertTSC22D4 (TSC22D4 Human)
UseTagsmNeonExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
8024 GFP_Kras_G12C_2A_puro
Plasmid#64373PurposeThis a retroviral expression plasmid expressing GFP tagged G12C mutant Kras 2A oncogene along with puro resistance geneDepositorInsertmouse Kras2A (Kras Mouse)
UseRetroviralTagsGFPExpressionMutationchanged glycine 12 to cysteinePromoterAvailable sinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-FLAG-SHLD1
Plasmid#114115PurposeExpresses N-terminally tagged FLAG-SHLD1 in mammalian cellsDepositorInsertSHLD1 (SHLD1 Human)
UseGenomic integration, tetracycline inducibleTagsFLAGExpressionMammalianMutationPromoterCMV/TetO2Available sinceAug. 28, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
8027_GFP-KrasG12C_2B_puro
Plasmid#64372PurposeThis a retroviral expression plasmid expressing GFP tagged G12C mutant Kras 2B oncogene along with puro resistance geneDepositorInsertmouse Kras2B (Kras Mouse)
UseRetroviralTagsGFPExpressionMutationchanged glycine 12 to cysteinePromoterAvailable sinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC52A3_STOP
Plasmid#161347PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC52A3 (C20orf54 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT/TO-V5-SHLD3
Plasmid#114124PurposeExpresses N-terminally tagged V5-SHLD3 in mammalian cellsDepositorInsertSHLD3 (SHLD3 Human)
UseGenomic integration, tetracycline inducibleTagsV5ExpressionMammalianMutationPromoterCMV/TetO2Available sinceAug. 23, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
8798 GFP-KrasG12C_2B_hygro
Plasmid#64376PurposeThis a retroviral expression plasmid expressing GFP tagged G12C mutant Kras 2B oncogene along with hygro resistance geneDepositorInsertmouse Kras2B (Kras Mouse)
UseRetroviralTagsGFPExpressionMutationchanged glycine 12 to cysteinePromoterAvailable sinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
8047_GFP-Kras2B_ires_puro
Plasmid#64371PurposeThis a retroviral expression plasmid expressing GFP tagged wild type Kras 2B oncogene along with puro resistance geneDepositorInsertmouse Kras2B (Kras Mouse)
UseRetroviralTagsGFPExpressionMutationnonePromoterAvailable sinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV-2 NSP13_nostop
Plasmid#149315PurposeGateway-compatible Entry vector, with insert of NSP13 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.DepositorInsertNSP13 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceMay 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221-LETMD1
Plasmid#132294PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertLETMD1 (LETMD1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 11, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221-ANKH_STOP
Plasmid#161444PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertANKH (ANKH Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
PET.SUMO eIF4G1 1-200
Plasmid#158791PurposeBacterial expression vector for the expression of N-terminal 6xHIS-SUMO-tagged codon optimized eIF4G1 amino acids 1-200 isoform lacking the microexon.DepositorInserteIF4G1 (codon optimized)
UseTags6xHIS and SUMOExpressionBacterialMutationamino acids 1-200PromoterT7Available sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV-2 NSP12_nostop
Plasmid#149314PurposeGateway-compatible Entry vector, with insert of NSP12 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.DepositorInsertNSP12 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceMay 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
lentiCRISPRv2_AMBRA1#2
Plasmid#174153PurposeLentiviral vector expressing Cas9 and a sgRNA against the human AMBRA1 geneDepositorInsertAMBRA (AMBRA1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-LETMD1_STOP
Plasmid#161460PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertLETMD1 (LETMD1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
lentiCRISPRv2_AMBRA1#1
Plasmid#174152PurposeLentiviral vector expressing Cas9 and a sgRNA against the human AMBRA1 geneDepositorInsertAMBRA (AMBRA1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
PET.SUMO eIF4G1 +MIC 1-200
Plasmid#158792PurposeBacterial expression vector for the expression of N-terminal 6xHIS-SUMO-tagged codon optimized eIF4G1 amino acids 1-200 isoform including the microexon.DepositorInserteIF4G1 (with microexon) codon optimized
UseTags6xHIS and SUMOExpressionBacterialMutationamino acids 1-200PromoterT7Available sinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 SARS-CoV-2 S 24nt-del
Plasmid#153952PurposeGateway-compatible Entry vector, with insert of S CDS bearing a 24nt deletion from SARS-CoV-2 genomic analysis in Davidson et al. 2020DepositorInsertS (S SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJune 23, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 SARS-CoV-2 S 24nt-del_nostop
Plasmid#153953PurposeGateway-compatible Entry vector, with insert of S CDS bearing a 24nt deletion from SARS-CoV-2 genomic analysis in Davidson et al. 2020DepositorInsertS (S SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJune 9, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 SARS-CoV-2 ORF7B C-trunc_nostop
Plasmid#153956PurposeGateway-compatible Entry vector, with insert of truncated ORF7B CDS from SARS-CoV-2 genomic analysis in Kim et al. 2020DepositorInsertORF7B (ORF7b SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJune 11, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223 SARS-CoV-2 ORF7B C-trunc
Plasmid#153957PurposeGateway-compatible Entry vector, with insert of truncated ORF7B CDS from SARS-CoV-2 genomic analysis in Kim et al. 2020DepositorInsertORF7B (ORF7b SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceJune 9, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYX-N-Puro-mNeon-TSC22D1
Plasmid#222667Purposeendogenously tag TSC22D1 with N-terminal mNeonDepositorInsertTSC22D1 (TSC22D1 Human)
UseTagsmNeonExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYX-N-Blast-miniTurbo-TSC22D2
Plasmid#222669Purposeendogenously tag TSC22D2 with N-terminal miniTurboDepositorInsertTSC22D2 (TSC22D2 Human)
UseTagsminiTurboExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-NRBP1-FL-N-FLAG
Plasmid#222675Purposeoverexpressing full length NRBP1 with N-terminal FLAGDepositorInsertNRBP1 (NRBP1 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-TSC22D2-FL-N-FLAG
Plasmid#222680Purposeoverexpressing full length TSC22D2 with N-terminal FLAGDepositorInsertTSC22D2 (TSC22D2 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-TSC22D2-FL-C-FLAG
Plasmid#222681Purposeoverexpressing full length TSC22D2 with C-terminal FLAGDepositorInsertTSC22D2 (TSC22D2 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pInducer-myc-ERK1-R84S/D338N
Plasmid#213594PurposeDoxycycline inducible expression of myc-tagged ERK1-R84S/D338N cDNA in mammilian cellsDepositorInsertMAPK3 (MAPK3 Human)
UseLentiviralTagsMYCExpressionMammalianMutationR84S, D338NPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pInducer-myc-ERK1-R84S/S170D/D338N
Plasmid#213595PurposeDoxycycline inducible expression of myc-tagged ERK1-R84S/S170D/D338N cDNA in mammilian cellsDepositorInsertMAPK3 (MAPK3 Human)
UseLentiviralTagsMYCExpressionMammalianMutationR84S, S170D, D338NPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only