We narrowed to 23,594 results for: promoter

1. pCDNA3-myc3-FBW5 delta F

   Plasmid

   #19912

   Depositor

   Yue Xiong

   Article

   Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

   Insert

   F-box and WD repeat domain containing 5 delta F (FBXW5 Human)
   

   Tags

   myc3

   Expression

   Mammalian

   Mutation

   deleted aa1-42 (F-box)

   Available Since

   May 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. NSP3C-Flag

   Plasmid

   #210349

   Purpose

   Expresses the C terminal of SARS-CoV-2 NSP3 fused with Flag in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   C terminal of SARS-CoV-2 NSP3 (ORF1ab )
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST-Tol2(Chien)-Krt4:Gal4VP16:tdTomato:5xUAS:HyPer:pA

   Plasmid

   #105643

   Purpose

   Co-expression of tdTomato and HyPer in differentiated keratinocytes of the periderm layer and ealy on also in the basal layer of the epidermis

   Depositor

   Sandra Rieger

   Article

   Lisse et al Proc Natl Acad Sci U S A. 2016 Apr 12;11

   Insert

   Krt4:Gal4VP16:tdTomato:5xUAS:HyPer:pA
   

   Expression

   Mammalian

   Available Since

   Feb. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

4. pDN-D2irC6kwh

   Plasmid

   #44725

   Depositor

   Gabor Balazsi

   Article

   Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1

   Inserts

   pCMV-D2i promoter
   mCherry
   

   Use

   Synthetic Biology; Expression regulator/reporter

   Tags

   Rabbit β-globin intron II and WPRE

   Expression

   Mammalian

   Mutation

   Initiator motif (Inr) displaced relative to pCMV-…

   Promoter

   pCMV-D2i

   Available Since

   April 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFP in pDBEcR-pIND(SP1)-DD-W/Bla

   Plasmid

   #201560

   Purpose

   Ecdysone-inducible vector with SB ITRs for expressing FLAG- EGFP-DD (WPRE in 3' UTR) and DBEcR (3xmyc-tagged); blasticidin resistance.

   Depositor

   Randy Poon

   Article

   Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754

   Insert

   DBEcR and EGFP
   

   Use

   Unspecified; Sleeping beauty transposon

   Tags

   DBEcR-myc, FLAG-EGFP, and FLAG-EGFP-DD

   Expression

   Mammalian

   Promoter

   EF1a and pIND(SP1)

   Available Since

   July 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. psiCHECK-2-TGFBR2-wt_3'UTR

   Plasmid

   #31882

   Depositor

   Robert Blelloch

   Article

   Subramanyam et al Nat Biotechnol. 2011 May;29(5):443-8. Ep

   Insert

   TGFBR2 wild-type 3'UTR (TGFBR2 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Sept. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

7. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_deltaC55G-hutRNApro_SV40polyA

   Plasmid

   #124542

   Purpose

   Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

   Depositor

   Tudor Fulga

   Article

   Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

   Insert

   deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_deltaC55G-hutRNApro
   

   Expression

   Mammalian

   Mutation

   d11-41 C55G, d11-41 C55G

   Promoter

   Minimal ADE

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pgRGFP

   Plasmid

   #82695

   Purpose

   Expresses gRNA of interest under U6 promoter (cloning by BpiI) and GFP under PGK promoter.

   Depositor

   Alan Mullen

   Article

   Daneshvar et al Cell Rep. 2016 Oct 4;17(2):353-365. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   PGK, U6

   Available Since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSCV-SMYD2 F184A

   Plasmid

   #85659

   Purpose

   retroviral expression of human SMYD2 F184A

   Depositor

   Julien Sage

   Article

   Reynoird et al Genes Dev. 2016 Apr 1;30(7):772-85. doi:

   Insert

   SMYD2 (SMYD2 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   F184A

   Promoter

   LTR

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. AYBEv0.1

    Plasmid

    #193966

    Purpose

    A wildtype adenine transversion base editor (AYBEv0.1, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    ecTadA8e-nSpCas9(D10A)-MPG(wt)
    

    Expression

    Mammalian

    Promoter

    hU6, EF1a, CBh

    Available Since

    Feb. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL4.24-Cd36-enhancer4

    Plasmid

    #138576

    Purpose

    Firefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17876439_17877752

    Depositor

    Lora Hooper

    Article

    Kuang et al Science. 2019 Sep 27;365(6460):1428-1434

    Insert

    Cd36 Promoter (Cd36 Mouse)
    

    Use

    Luciferase

    Promoter

    Minimal promoter

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFAB3677

    Plasmid

    #47833

    Purpose

    BIOFAB RFP reporter plasmid for measuring promoter 2 + BCD1 efficiency.

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    promoter 2 and BCD1
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    see insert

    Available Since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR RPS27A K107/113R-3xFlag_IRES-GFP

    Plasmid

    #198392

    Purpose

    RPS27A K107/113R (ubiquitin/ribosomal protein eS31 fusion) expression

    Depositor

    Jack Taunton

    Article

    Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

    Insert

    RPS27A (RPS27A Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    ubiquitination site double mutant (K107/113R)

    Promoter

    EF1A

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. ATP1A1_G6_T804N_Dual_pegRNA

    Plasmid

    #173200

    Purpose

    Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 T804N-G6 pegRNA with a user-specified pegRNA from two independent U6 promoters. pU6-pegRNA-GG-acceptor-like plasmid

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G6 T804N pegRNA
    

    Use

    CRISPR; Prime editing

    Expression

    Mammalian

    Promoter

    Tandem U6 promoters

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFRTpsiCHECK

    Plasmid

    #154454

    Purpose

    Expression of renilla luciferase and firefly luciferase

    Depositor

    Markus Landthaler

    Article

    Zinnall et al Nat Commun. 2022 May 18;13(1):2727. doi:

    Insert

    Renilla luciferase, firefly luciferase
    

    Available Since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

    Plasmid

    #124543

    Purpose

    Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
    

    Expression

    Mammalian

    Mutation

    d11-41 C55G, d11-41

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-shNCLX-2

    Plasmid

    #181871

    Purpose

    Expresses NCLX-targeted shRNA under control of the U6 promoter and mCherry under control of the CaMKIIa promoter

    Depositor

    Hilmar Bading

    Article

    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

    Insert

    solute carrier 8 family member B1 (Slc8b1 Mouse)
    

    Use

    AAV, Adenoviral, and Mouse Targeting

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-shNCLX-1

    Plasmid

    #181870

    Purpose

    Expresses NCLX-targeted shRNA under control of the U6 promoter and mCherry under control of the CaMKIIa promoter

    Depositor

    Hilmar Bading

    Article

    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

    Insert

    solute carrier 8 family member B1 (Slc8b1 Mouse)
    

    Use

    AAV, Adenoviral, and Mouse Targeting

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFAB3833

    Plasmid

    #47839

    Purpose

    BIOFAB RFP reporter plasmid for measuring promoter 8 + BCD1 efficiency.

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    promoter 8 and BCD1
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    see insert

    Available Since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL2 p15 (-756/+68) SBR2 Mut 3' box

    Plasmid

    #15711

    Depositor

    Joan Massague

    Article

    Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

    Insert

    p15 promoter (CDKN2B Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Mut 3' box in SBR2. The 3' box contains…

    Available Since

    Sept. 7, 2007

    Availability

    Academic Institutions and Nonprofits only