We narrowed to 7,715 results for: ski
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Plasmid#179081PurposeNOTE: An improved version of this plasmid is now available. See Addgene plasmid #242072. All-in-one prime editor piggyBac transposon, SpG variantDepositorInsertSpCas9 SpG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
UseCRISPR and Synthetic Biology; Piggybac transposonTagsSV40 NLSExpressionMammalianMutationD1135L, S1136W, G1218K, E1219Q, R1335Q, T1337RPromoterCAGAvailable SinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3-pSMANTIS_1kb_KLF2mut 449,548,732,899,884 (All)
Plasmid#194190PurposeIncludes the promoter (1kb) of SMTS with 5 mutated KLF binding sites (positions 449,548,732,899,884)DepositorInsertSMANTIS promoter (1kb) (SMANTIS Human)
UseLuciferaseMutation5 mutated KLF binding sites (positions 449,548,73…PromoterSMANTIS promoter (1kb), mutatedAvailable SinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-pSMANTIS_1kb_KLF2mut 899
Plasmid#194193PurposeIncludes the promoter (1kb) of SMTS with mutated KLF binding sites (position 899)DepositorInsertSMANTIS promoter (1kb) (SMANTIS Human)
UseLuciferaseMutationmutated KLF binding sites (position 899)PromoterSMANTIS promoter (1kb), mutatedAvailable SinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMBP-parallel1-GK5_v2
Plasmid#134288PurposeExpresses GK5 (isoform 2) in bacteriaDepositorInsertGk5 (Gk5 Mouse)
TagsMBPExpressionBacterialMutationmouse Gk5 isoform 2; 534 amino acidsPromoterT7Available SinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCEFL GAL4dbd-KLF4 1-329
Plasmid#140146PurposeGAL4-DNA binding domain fusion protein with the 329 N-terminal amino acids of human KLF4DepositorInsertKLF4 Kruppel factor (KLF4 Human)
TagsGAL4dbdExpressionMammalianMutationDeleted amino acids 330 to 479PromoterEF1aAvailable SinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_RBM45_WT_V5
Plasmid#82968PurposeGateway Donor vector containing RBM45, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_STC2_WT_V5
Plasmid#82957PurposeGateway Donor vector containing STC2, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCEFL GAL4dbd-KLF4 1-156
Plasmid#140147PurposeGAL4-DNA binding domain fusion protein with the 156 N-terminal amino acids of human KLF4 (activation domain)DepositorInsertKLF4 aa 1-156 (KLF4 Human)
TagsGAL4dbdExpressionMammalianMutationDeleted amino acids 157 to 479PromoterEF1aAvailable SinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_NOSIP_WT_V5
Plasmid#82970PurposeGateway Donor vector containing NOSIP, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_PSMD9_WT_V5
Plasmid#82950PurposeGateway Donor vector containing PSMD9, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBABE-hygro-BAP1-G312G-HA
Plasmid#154022PurposeMammalian Expression of HA-tagged BAP1 with the Synonymous Mutation p.G312GDepositorInsertBAP1 (BAP1 Human)
UseRetroviralTagsHemagglutinin (HA)ExpressionMammalianMutationc.936T>G, p.G312G (Synonymous Mutation) by Sit…Available SinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.S72A
Plasmid#106185PurposeWeak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas ServiceAAV1InsertSF-Venus-iGluSnFR.S72A
UseAAVMutationSFGFP: T203Y, F46L, T65G, S72A GltI: S72APromoterhSynapsinAvailable SinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPHR-EYFP-hPMLIII
Plasmid#103825PurposeSoluble BLInCR effector that is recruited to 'localizer' sites upon blue light illuminationDepositorAvailable SinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX RASGAP(C)-SH2
Plasmid#46431DepositorInsertGap/RASGAP(C) (RASA1 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 339-452PromoterTacAvailable SinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_BRP44_WT_V5
Plasmid#82976PurposeGateway Donor vector containing BRP44, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO-Tet-On-PLXNB2-shRNA2
Plasmid#98400PurposeLentivirus for expression of shRNA2 against human PLEXIN-B2 (Dox-inducible)DepositorInsertPlexin-B2 (PLXNB2 Human)
UseLentiviralAvailable SinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX RASGAP(NC)-SH2
Plasmid#46433DepositorInsertGap/RASGAP(NC) (RASA1 Human)
TagsGSTExpressionBacterialMutationcontains amino acids 171-448PromoterTacAvailable SinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
W118-1_hRSK3(RPS6KA2)_K91A_K444A
Plasmid#180385Purposelentiviral transduction of human RSK3 (RPS6KA2) gene with point mutations (K91A and K444A) that inactivate kinase activityDepositorInsertRPS6KA2_K91A/K444A (RPS6KA2 Human)
UseLentiviralExpressionMammalianMutationK91A/K444APromoterCMVAvailable SinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_RBM45_p.D434Y
Plasmid#82758PurposeGateway Donor vector containing RBM45 , part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_RBM45_p.M126I
Plasmid#82759PurposeGateway Donor vector containing RBM45 , part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only