We narrowed to 15,944 results for: POR
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Plasmid#138203PurposeEncodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.DepositorInsertPKAsub-YPet-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Myc-CD4-mCherry-HA-FRB
Plasmid#100740PurposeTo express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosisDepositorInsertCD4
UseTagsFRB, HA, Myc, and mCherryExpressionMammalianMutationEngineered to remove large part of extracellular …PromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pU6_T7_INT[6xminimal_Puf1]
Plasmid#107608PurposeTransient expression of an "INT" construct [bearing six minimal Puf1 stem-loops; 'INT' design: Shechner et al., Nature Methods 2015], in mammalian cells, U6 promoterDepositorInsert6x minimal Puf1 motif
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterU6Available sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIhh742bp-Luc
Plasmid#53605PurposeReporter construct containing a 742 bp promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assaysDepositorInsertIhh promoter
UseLuciferase; ReporterTagsExpressionMutationPromoterAvailable sinceJune 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-spider-monkey-retroCHMP3-HA
Plasmid#154179Purposeexpresses spider monkey retroCHMP3 in mammalian cellsDepositorInsertretroCHMP3
UseTagsHAExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3-JNKsubDD-YPet-NES
Plasmid#84633PurposeEncodes C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertJNKsubDD-YPet-NES
UseTags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMIII-Neo-E2Crimson
Plasmid#153425PurposeRetroviral (MSCV) expression of E2-Crimson fluorescent proteinDepositorInsertE2-Crimson
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-mU6-Luc2GO-PGK-Neo
Plasmid#136905PurposeLentivirus for base editing activatable Luciferase2 expression in mammalian cells. All in one vector with sgLuc2GO.DepositorInsertLuc2CyGo
UseLentiviralTagsExpressionMutationATG>ACGPromoterSFFVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSK226
Plasmid#129268Purposedual yTRAP, RNQ1 fusion to aTF2 reporting on [RNQ+] state with mNeonGreen reporterDepositorInsertRnq1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
XW20
Plasmid#65836PurposePunc-4-QF-3'UTR-unc-54 (The endogenous SphI, KpnI and SacI sites in QF are removed, and no SL2-mcherry)DepositorInsertOR74A quinic acid utilization activator (qa-1F)
UseTagsExpressionWormMutationThe endogenous SphI, KpnI and SacI sites in QF ar…PromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
FLAG-SLC30A10-E25A
Plasmid#82345PurposeExpresses FLAG-tagged SLC30A10-E25A in mammalian cellsDepositorInsertSLC30A10 (SLC30A10 Human)
UseTags3xFLAGExpressionMammalianMutationE25APromoterCMVAvailable sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-owl-monkey-retroCHMP3(148)-HA
Plasmid#154252Purposeexpresses prematurely truncated owl monkey retroCHMP3 in mammalian cellsDepositorInsertretroCHMP3
UseTagsHAExpressionMammalianMutationC-terminal truncation of retroCHMP3 at amino acid…PromoterEF1-alphaAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-h-mmPtch1-B
Plasmid#120888PurposeExpresses Ptch1-B for function assaysDepositorInsertPtch1 (Ptch1 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJL2
Plasmid#184130PurposeFor rescuing null mrp-1 mutation and labelling mrp-1 protein. Translational fusion construct. pPD95.75_Pmrp-1::mrp-1(isoform C cDNA)::gfp.DepositorInsertMRP-1 (mrp-1 Nematode)
UseTagsGFPExpressionWormMutationPromoterPmrp-1Available sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJL1
Plasmid#184129PurposeFor rescuing null mrp-1 mutation. pPD95.77_Pmrp-1::mrp-1(isoform C cDNA)::SL2::gfp. SL2 prevents fusion of MRP-1 and GFP.DepositorInsertMRP-1 (mrp-1 Nematode)
UseTagsGFPExpressionWormMutationPromoterPmrp-1 (3kb)Available sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWZLblasti-CTR1M154A
Plasmid#53158PurposeExpresses human CTR1 M154A from blasticidin resistance retroviral vectorDepositorInsertCTR1 (SLC31A1 Human)
UseRetroviralTagsExpressionMammalianMutationM154APromoterAvailable sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGREAT17
Plasmid#170905PurposeGoldenBraid Double Transcriptional Unit - p35SoTMV_E-Luc_AtHSP18.2t+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…Promoterp35SoTMVAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-CmTMC1truncated
Plasmid#154908PurposeFor the expression and purification of the truncated TMC1 protein from Chelonia mydas. The protease cleavage site was replaced with the HRV 3C site.DepositorInsertGFP-CmTMC1truncated
UseTags8histidine-EGFPExpressionInsectMutationTruncations of the residues 1-243 and 843-969PromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-MuTMC2truncated
Plasmid#154909PurposeFor the expression and purification of the truncated TMC2 protein from Melopsittacus undulates. The protease cleavage site was replaced with the HRV 3C site.DepositorInsertGFP-MuTMC2truncated
UseTags8histidine-EGFPExpressionInsectMutationTruncations of the residues 1-179 and 691-716PromoterAvailable sinceJuly 27, 2020AvailabilityAcademic Institutions and Nonprofits only