We narrowed to 9,442 results for: pho

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pAAV-GFAP104-ChromeT-mCherry

Plasmid

#123126

Purpose

AAV-mediated expression of ChromeT-mCherry under the GFAP promoter.

Depositor

Edward Boyden

Article

Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

Insert

ChromeT-mCherry

Use

AAV

Tags

mCherry

Expression

Mammalian

Mutation

Channelrhodopsin-2 (ChR2) with mutations A71S/ E9…

Promoter

GFAP104

Available Since

April 1, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-mEGFP(A206K)-paCaMKII(I539E)-ShadowG

Plasmid

#167478

Purpose

Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

mEGFP(A206K)-paCaMKII(I539E)-ShadowG

Tags

ShadowG and mEGFP(A206K)

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-mEGFP(A206K)-paCaMKII(C450A)-ShadowG

Plasmid

#167479

Purpose

Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

mEGFP(A206K)-paCaMKII(C450A)-ShadowG

Tags

ShadowG and mEGFP(A206K)

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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pBz172_CsoS2_N1_GFP

Plasmid

#140852

Purpose

His-tagged first N-peptide of NTD fused to Superfolder GFP ([N1]-GFP) used for BLI and MST, pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 NTD repeat 1 fused to GFP

Tags

Hexahistidine tag, Strep-Tag II, and Superfolder …

Expression

Bacterial

Promoter

T7

Available Since

June 19, 2020

Availability

Academic Institutions and Nonprofits only

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pBz175_CsoS2_N4_GFP

Plasmid

#140855

Purpose

His-tagged fourth N-peptide of NTD fused to Superfolder GFP ([N4]-GFP) used for BLI, pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 NTD repeat 4 fused to GFP

Tags

Hexahistidine tag, Strep-Tag II, and Superfolder …

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBz173_CsoS2_N2_GFP

Plasmid

#140853

Purpose

His-tagged second N-peptide of NTD fused to Superfolder GFP ([N2]-GFP) used for BLI, pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 NTD repeat 2 fused to GFP

Tags

Hexahistidine tag, Strep-Tag II, and Superfolder …

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBz174_CsoS2_N3_GFP

Plasmid

#140854

Purpose

His-tagged third N-peptide of NTD fused to Superfolder GFP ([N3]-GFP) used for BLI, pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 NTD repeat 3 fused to GFP

Tags

Hexahistidine tag, Strep-Tag II, and Superfolder …

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLz26_CsoS2_N1-N2_GFP

Plasmid

#140851

Purpose

His-tagged first two N-peptides of NTD fused to Superfolder GFP ([N1-N2]-GFP) used for MST, pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 NTD repeats 1-2 fused to GFP

Tags

Hexahistidine tag, Strep-Tag II, and Superfolder …

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pTAMACHis6_PiuD

Plasmid

#128953

Purpose

expresses PiuD in E.coli

Depositor

James Naismith

Article

Luscher et al Antimicrob Agents Chemother. 2018 May 25

Insert

Putative outer membrane receptor for iron transport (PiuD)

Tags

6xHis and TamA

Expression

Bacterial

Mutation

Signal peptide sequence has been removed (1-105)

Promoter

T7

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pMH-HT_SsCHAD-H29A-R32A-R36A

Plasmid

#127786

Purpose

Expresses a SsCHAD mutant protein in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

SsCHAD (SULM_RS11045 Sulfolobus solfataricus)

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

mutations in His29, Arg32 and Arg36 to Ala

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pMH-HT_CtCHAD-R438A-K441A

Plasmid

#127791

Purpose

Expresses a CtCHAD mutant protein in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

CtCHAD (CT0884 Chlorobium tepidum)

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

Residues 208-522 (CHAD domain), mutations in Arg4…

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pMH-HT_ygiF-TTM

Plasmid

#127794

Purpose

Expresses the TTM/CYTH domain of ygiF in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

ygiF (ygiF Escherichia coli)

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

N-terminal TTM domain of ygiF (residues 1-200)

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3-cytXlATL2-R232Q

Plasmid

#124064

Purpose

Control protein. The R232Q mutation abolishes dimerization activity, so this construct is inactive compared to wild type cytATL

Depositor

Tom Rapoport

Article

Wang et al J Cell Biol. 2013 Dec 9;203(5):801-14. d

Insert

cytXlATL2-R232Q

Expression

Bacterial

Promoter

tac

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pJet-CMV-CD5cmyc2-bglpA

Plasmid

#110645

Purpose

Expression plasmid for c-Myc-tag embedded into human CD52 with short polyA

Depositor

Dmitriy Mazurov

Article

Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

Insert

CMV-CDcmyc2-bglpA

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-mCherry-FHA1-subT391A-mCherry

Plasmid

#104462

Purpose

Cre-dependent, Non-phosphorylatable PKA consensus substrate tagged with mCherry

Depositor

Bernardo Sabatini

Article

Chen et al Neuron. 2017 Dec 6;96(5):1070-1083.e5. d

Insert

mCherry-subT391A-FHA1-mCherry

Use

AAV

Tags

mCherry

Promoter

CAG

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pSC27

Plasmid

#104801

Purpose

CRISPR/Cas9 2xplex gRNA targeting Medtr4g020620 (Pho2ab). Also expresses Cas9 from Gmubi promoter.

Depositor

Nevin Young

Article

Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

Insert

Medtr4g020620

Use

CRISPR

Expression

Plant

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pRSM13

Plasmid

#101644

Purpose

Encodes the fluorescent protein meleCFP for constitutive expression in E. coli.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

meleCFP

Tags

6XHis

Expression

Bacterial

Available Since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pBAD-Tam1

Plasmid

#101639

Purpose

Encodes the fluorescent protein Tam1 for arabinose-inducible expression in E. coli.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

Tam1

Tags

6XHis

Expression

Bacterial

Available Since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pThom77.6

Plasmid

#101637

Purpose

Encodes the fluorescent protein amFP486/K68M under a CMV promoter.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

amFP486/K68M

Expression

Mammalian

Mutation

K68M

Promoter

CMV

Available Since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pThom77.3

Plasmid

#101636

Purpose

Encodes the fluorescent protein amFP486/K68M for constitutive expression in E. coli.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

amFP486/K68M

Tags

6XHis

Expression

Bacterial

Mutation

K68M

Available Since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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