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pNCS YFP

Plasmid

#91759

Purpose

Bacterial expression of a fluorescent protein, Yellow Fluorescent Protein (YFP)

Depositor

Erik Rodriguez, Roger Tsien

Article

Cubitt et al Trends Biochem Sci. 1995 Nov;20(11):448-

Insert

Venus YFP (A206K)

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Promoter

T7

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pPGxRF3

Plasmid

#138384

Purpose

T2A-GAL4 knock-in cassette

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

T2A-GAL4

Expression

Insect

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g5

Plasmid

#80788

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 5/15/25 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Promoter

U6

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pSEED-sfGFP:ALFA

Plasmid

#219642

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP:ALFA using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

sfGFP:ALFA SEED Cassette

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pBT378_pattB-pCA-GFP-pA-attB

Plasmid

#52554

Purpose

for PhiC31 integrase-mediated cassette exchange

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP

Expression

Mammalian

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g6

Plasmid

#80789

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 6/16/26 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Promoter

U6

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pDZ416 (24PP7SL loxP-Kan-loxP)

Plasmid

#45163

Depositor

Robert Singer, Daniel Zenklusen

Article

Hocine et al Nat Methods. 2013 Feb;10(2):119-21. doi:

Insert

24xPP7 Stem Loop Cassette

Use

Cre/Lox

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g7

Plasmid

#80790

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 7/17/27 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Promoter

U6

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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PCT62

Plasmid

#170391

Purpose

expresses three sgRNA cassettes

Depositor

Jonathan Weissman

Article

Quinn et al Science. 2021 Feb 26;371(6532). pii: sci

Insert

PCT62-targetsite

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g9

Plasmid

#80792

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 9/19/29 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Promoter

U6

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pMA-SpCas9-g8

Plasmid

#80791

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 8/18/28 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Promoter

U6

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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EC70364

Plasmid

#209515

Purpose

Cloning in complete guide expression cassettes, with Cas9

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pET-HA-MBP-CatN-TEV-CfaC-eGFP-FLAG

Plasmid

#241423

Purpose

Protein Transposition Cassette Containing CatN and CfaC

Depositor

Thomas Muir

Article

Hua et al Science. 2025 Apr 4;388(6742):68-74. doi

Insert

His5-Sumo-HA-MBP-CatN-TEV-CfaC-eGFP-FLAG

Tags

His5, HA

Expression

Bacterial

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pJH136

Plasmid

#47554

Purpose

pJH136 carries a URA3 cassette flanked by PCR priming sites U2 and D2. URA3 was derived from S288c and extends from 243 bp upstream of the start codon to 79 bp downstream of the stop codon.

Depositor

Ronald Davis

Article

Horecka et al Yeast. 2014 Mar;31(3):103-12. doi: 10.10

Insert

URA3

Use

Pcr template

Promoter

Native URA3 promoter, -243 to -1.

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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EC67841

Plasmid

#209512

Purpose

Cloning in complete guide expression cassettes, with Cas12a (D156R)

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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EC64434

Plasmid

#209518

Purpose

Cloning in complete guide expression cassettes, with Cas9 & Cas12a(D156R)

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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LentiGuide-BC-EF1a-Puro

Plasmid

#127170

Purpose

PCR template for EF1a-puro resistance cassette

Depositor

Paul Blainey

Article

Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

Insert

None

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pEM351

Plasmid

#248763

Purpose

80xTetO Sponge Plasmid with BsaI sites flanking sponge casette for Golden Gate Assembly

Depositor

Carolina Tropini

Article

McCallum et al Cell. 2026 Feb 19;189(4):1245-1261.e21.

Insert

T7Te-rrnBT1-80xTetO

Expression

Bacterial

Available Since

Jan. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pEM414

Plasmid

#248766

Purpose

20xTetO Sponge Plasmid with BsaI sites flanking sponge casette for Golden Gate Assembly

Depositor

Carolina Tropini

Article

McCallum et al Cell. 2026 Feb 19;189(4):1245-1261.e21.

Insert

T7Te-rrnBT1-20xTetO

Expression

Bacterial

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pHMattR3-attR4-GFP

Plasmid

#196247

Purpose

pHM1 derived plasmid containing the Gateway cassette attR3-attR4 and GFP gene

Depositor

Bing Yang

Article

Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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