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pFLIP42

Plasmid

#65617

Purpose

FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

Depositor

Wolf Frommer

Article

Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

Type

Empty backbone

Tags

6His-Cit-attR1 and attR2-mCer-cMyc

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-cyEGFP-6XDSR

Plasmid

#49865

Purpose

for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with 6 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

yEGFP

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-cyEGFP-3XDSR

Plasmid

#49863

Purpose

for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with 3 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

yEGFP

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-c3HA-spo5DSR

Plasmid

#49872

Purpose

for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

3XHA

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-c3HA-6XDSR

Plasmid

#49870

Purpose

for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 6 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

3XHA

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-c3HA-3XDSR

Plasmid

#49868

Purpose

for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 3 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

3XHA

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-cyEGFP-8XDSR

Plasmid

#49866

Purpose

for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with 8 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

yEGFP

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-cyEGFP-4XDSR

Plasmid

#49864

Purpose

for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with 4 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

yEGFP

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-c3HA-8XDSR

Plasmid

#49871

Purpose

for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 8 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

3XHA

Available Since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-c3HA-4XDSR

Plasmid

#49869

Purpose

for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 4 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

3XHA

Available Since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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KaiC CII cat-/EE (E318Q; S431E; T432E)

Plasmid

#42498

Depositor

Michael Rust

Article

Phong et al Proc Natl Acad Sci U S A. 2013 Jan 15;11

Insert

KaiC CII cat-/EE (E318Q; S431E; T432E)

Expression

Bacterial

Mutation

E318Q; S431E; T432E

Promoter

t7

Available Since

March 4, 2013

Availability

Academic Institutions and Nonprofits only
KaiC CI cat- (E77Q;E78Q)

Plasmid

#41886

Depositor

Michael Rust

Article

Phong et al Proc Natl Acad Sci U S A. 2013 Jan 15;11

Insert

KaiC CI cat -(E77Q;E78Q)

Expression

Bacterial

Mutation

changed glutamic acid 77 and 78 to glutamine

Promoter

t7 promoter

Available Since

March 4, 2013

Availability

Academic Institutions and Nonprofits only
KaiC CII cat- (E318Q)

Plasmid

#41887

Depositor

Michael Rust

Article

Phong et al Proc Natl Acad Sci U S A. 2013 Jan 15;11

Insert

Kai C CII cat (E318Q)

Use

Bl de3 21

Expression

Bacterial

Mutation

changed amino acid 318 E to Q

Promoter

t7

Available Since

March 4, 2013

Availability

Academic Institutions and Nonprofits only
pBS-L1PA1-CH-mneo

Plasmid

#51288

Purpose

contains the fully codon optimized ORF1 and ORF2 of the human L1RP

Depositor

Astrid Roy-Engel

Article

Wagstaff et al PLoS One. 2011 May 10;6(5):e19672. doi:

Insert

L1 (L1RE1 Human)

Tags

mneoI indicator cassette containing an inverted n…

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pRDA_887

Plasmid

#201164

Purpose

Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65 and NLS

Depositor

John Doench

Article

Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

Insert

Puromycin resistance

Use

CRISPR and Lentiviral

Promoter

EFS

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCI SMN2

Plasmid

#72287

Purpose

SMN2 exon 7 splicing cassette

Depositor

Elliot Androphy

Article

Lorson et al Proc Natl Acad Sci U S A. 1999 May 25;96

Insert

SMN2 (SMN2 Human)

Expression

Mammalian

Mutation

contains exons 6-7-8 including introns, multiple …

Promoter

CMV

Available Since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pLP2

Plasmid

#209989

Purpose

Entry vector to store Level 0 resistance cassette parts and E. coli shuttle part (containing no E. coli ori)

Depositor

Georg Fritz, Thorsten Mascher

Article

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pMo130-TelR

Plasmid

#50799

Purpose

Suicide vector for generating chromosomal deletions in multidrug-resistant bacteria

Depositor

Kim Lee Chua

Article

Amin et al BMC Microbiol. 2013 Jul 13;13:158. doi:

Insert

tellurite-resistance cassette

Use

Suicide vector

Promoter

PCs12

Available Since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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pKVL2-U6gRNA_SAM(BbsI)-PGKpuroBFP-W

Plasmid

#112925

Purpose

Lentiviral vector for SAM-CRISPRa gRNA expression with puroBFP

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

hU6 promoter, gRNA-SAM expression cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMS82

Plasmid

#68110

Purpose

Integration vector derived from Streptomyces phage phiBT1. Hygromycin resistance gene, oriT, ColEi origin of replication

Depositor

Maggie Smith

Article

Gregory et al J Bacteriol. 2003 Sep;185(17):5320-3.

Inserts

aac3(IV)
Hyg
oriT
attP
int

Use

Streptomyces integrating vector

Mutation

Fragment

Available Since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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