We narrowed to 8,596 results for: Set

1. pSL3713 (pTnsC_Pse)

   Plasmid

   #200900

   Purpose

   Expresses human codon-optimized Pseudoalteromonas TnsC from a CMV promoter.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   PseTnsC
   

   Tags

   3xNLS

   Expression

   Mammalian

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNCS mHoneydew

   Plasmid

   #91760

   Purpose

   Bacterial expression of a fluorescent protein, monomeric Honeydew (mHoneydew)

   Depositor

   Erik Rodriguez, Roger Tsien

   Article

   Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

   Insert

   monomeric Honeydew
   

   Tags

   no tags

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. EKAREN5-gl

   Plasmid

   #225958

   Purpose

   The YPet and mTurquoise sequence cassettes in EKAREN5(Addgene 167821) were replaced with a synonymous codon variant of YPet and mTurquoise-gl and subcloned into pCSII lentiviral backbone.

   Depositor

   Toru Hiratsuka

   Article

   Weisheng et al Cell Struct Funct. 2024 Dec 18. doi: 10.

   Insert

   EKAREN5-gl
   

   Use

   Lentiviral

   Tags

   nls localization motif

   Expression

   Mammalian

   Promoter

   EF-1-alpha promoter

   Available Since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDGB3_alpha1-pAtUBQ10:HA-LRR-NEL-mcherry-Tnos

   Plasmid

   #211840

   Purpose

   Plant transient expression cassette for the catalytically inactive E3-DART ligase under a UBQ10 promoter.

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Insert

   LRR-NEL
   

   Use

   Synthetic Biology

   Tags

   HA and mCherry

   Expression

   Plant

   Promoter

   UBIQUITIN10

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKVL2-U6gRNA_SAM(BbsI)-PGKpuroBFP-W

   Plasmid

   #112925

   Purpose

   Lentiviral vector for SAM-CRISPRa gRNA expression with puroBFP

   Depositor

   Kosuke Yusa

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   hU6 promoter, gRNA-SAM expression cassette
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-TET

   Plasmid

   #20909

   Purpose

   piggyBac empty vector with Gateway cassette, tet inducible promoter, and linked reporter (IRES-bgeo)

   Depositor

   Andras Nagy

   Article

   Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

   Insert

   Gateway Destination Vector
   

   Use

   piggybac

   Tags

   IRES-bGeo

   Expression

   Mammalian

   Available Since

   May 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. MXS_PGK::ZeoR-bGHpA

   Plasmid

   #62442

   Purpose

   MXS_chaining vector with PGK::ZeoR-bGHpA

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   resistance cassette against Zeocin with PGK Promoter
   

   Use

   Synthetic Biology

   Available Since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMVV104

   Plasmid

   #215310

   Purpose

   Expresses sfGFP under induction with the arabinose Marionette Cassette (AraCAM + araE + PBAD-sfGFP) with a BBR1 backbone.

   Depositor

   Brian Pfleger

   Article

   Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

   Insert

   sfGFP
   

   Use

   Synthetic Biology

   Tags

   None

   Expression

   Bacterial

   Promoter

   PAraBAD

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDGB3_alpha1-pAtUBQ10:HA-LRR-NELC492A-mcherry-Tnos

   Plasmid

   #211843

   Purpose

   Plant transient expression cassette for the E3-DART ligase under a UBQ10 promoter.

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Insert

   LRR-NEL
   

   Use

   Synthetic Biology

   Tags

   HA and mCherry

   Expression

   Plant

   Mutation

   C492A

   Promoter

   UBIQUITIN10

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZH23 Dual G-less containing ySSA1 polyA

    Plasmid

    #231647

    Purpose

    yeast S.cerevisiae SSA1 polyA termination region inserted between the dual G-less cassette

    Depositor

    Jesper Svejstrup

    Article

    Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

    Insert

    SSA1 polyA termination region
    

    Use

    Synthetic Biology

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZH20 Dual G-less containing yCYC1 polyA

    Plasmid

    #231646

    Purpose

    yeast S.cerevisiae CYC1 polyA termination region inserted between the dual G-less cassette

    Depositor

    Jesper Svejstrup

    Article

    Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

    Insert

    CYC1 polyA termination region
    

    Use

    Synthetic Biology

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. Halo-ATG16L1 HRD

    Plasmid

    #207537

    Purpose

    Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG16L1 locus in human cells

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    HaloTag with internal PuroR cassette flanked by human ATG16L1 locus sequences
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    Endogenous

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pC018 - LshCas13a from Leptotrichia shahii

    Plasmid

    #91908

    Purpose

    Expresses LshCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LshCas13a and guide expression cassette
    

    Expression

    Bacterial

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBDK

    Plasmid

    #135190

    Purpose

    Integration and excision of kanamycin cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection

    Depositor

    Hongqing Fang

    Article

    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

    Insert

    KanR
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    cat

    Available Since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. BPK2660

    Plasmid

    #70709

    Purpose

    Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGSKU

    Plasmid

    #72243

    Purpose

    Contains the CORE cassette with convergent KlURA3 and KanMX4 markers and the I-SceI gene under the inducible GAL1 promoter

    Depositor

    Michael Resnick, Francesca Storici

    Article

    Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

    Inserts

    KlURA3
    kanMX4
    I-SceI
    

    Expression

    Bacterial

    Available Since

    Jan. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSLS.492

    Plasmid

    #184957

    Purpose

    Test effect of a1/a2 length on editing rpoB using retron recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 22
    

    Expression

    Bacterial

    Mutation

    rpoB donor T1534C, a1/a2 length extended to 22 bp

    Promoter

    T7/lac

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. miReporter-CAG

    Plasmid

    #82478

    Purpose

    microRNA reporter relying on a bidirectional CAG-based promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi

    Insert

    H2B-mCherry and H2B-Citrine. HygroR
    

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pXL004-ishRNA-scramble

    Plasmid

    #36311

    Depositor

    Sean Palecek

    Article

    Lian et al Proc Natl Acad Sci U S A. 2012 May 29.

    Insert

    shRNA of scramble
    

    Use

    Lentiviral and RNAi

    Tags

    EF1a-TetR-IRES-RFP-Puro

    Expression

    Mammalian

    Promoter

    TetO-H1

    Available Since

    July 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pL1P4 ZmUbiP:GRF-GIF:NosT

    Plasmid

    #198048

    Purpose

    MoClo Golden Gate Level 1 Position 4. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.

    Depositor

    Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing

    Insert

    ZmUbiP::TaGRF4-GIF1::NosT
    

    Use

    Synthetic Biology

    Promoter

    Zea mays (maize) ubiquitin promoter

    Available Since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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