We narrowed to 146,921 results for: ins;

1. pBVboostFGII WPRE 2A-CVB3

   Plasmid

   #202076

   Purpose

   Bacterial expression of 2A protease from coxsackievirus B3 (CVB3). Contains C-terminal His6-tag.

   Depositor

   Vesa Hytönen

   Article

   Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

   Insert

   CVB3 2A protease with HisTag
   

   Use

   Tags

   6xHis-tag

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   polh, CAG, T7

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. sgVRK1-hygro

   Plasmid

   #199640

   Purpose

   sgRNA guide against VRK1

   Depositor

   William Hahn

   Article

   So et al JCI Insight. 2022 Oct 10;7(19):e158755.

   Insert

   N/A (VRK1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET32a-hNRG1a

   Plasmid

   #199235

   Purpose

   Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

   Depositor

   Nilson Zanchin

   Article

   Ferreira et al Mol Biol Rep. 2022 Dec;49(12):11715-1172

   Insert

   Neuregulin (NRG1 Human)
   

   Use

   Tags

   TrxA-6xHis-S-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAc5.1B-EGFP-DmNanos-del50-236_AC

   Plasmid

   #148459

   Purpose

   Insect Expression of DmNanos-del50-236

   Depositor

   Elisa Izaurralde

   Article

   Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

   Insert

   DmNanos-del50-236 (nos Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   sequence contains a deletion of N283 and a mutati…

   Promoter

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGTVL2-BIRC2-BIR3

   Plasmid

   #196367

   Purpose

   protein expression of the GST-tagged BIR3 domain

   Depositor

   Stefan Knapp

   Article

   Schwalm et al Front Cell Dev Biol. 2022 May 26;10:8865

   Insert

   BIRC2 BIR3 domain
   

   Use

   Tags

   His6-GST-TEV

   Expression

   Bacterial

   Mutation

   contains only S260-Y352

   Promoter

   T7

   Available Since

   Feb. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. YW347_Ptbb2UTR-loxP-RFP-miniMOS

   Plasmid

   #196064

   Purpose

   a plasmid modified from miniMOS vector pCFJ910. the modification including insertions of myo2p::TagRFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and tagRFP cassettes.

   Depositor

   Wenxing Yang

   Article

   Li et al PeerJ. 2023 May 17;11:e15433. doi: 10.77

   Inserts

   myo2p driven tagRFP
   NeoR
   Minimal Mos1
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   myo-2

   Available Since

   Jan. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. deltaVP1d63C + VP2C-GFPdeg

   Plasmid

   #182481

   Purpose

   YIp for expressing Murine polyomavirus deltaVP1 with the 63 C-ter residues truncated (GAL1 promoter) and destabilised GFP tagged with VP2C (GAL10 promoter). Contains uracil marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al bioRxiv 2022.11.24.517869

   Inserts

   VP2C-GFPdeg
   deltaVP1d63C
   

   Use

   Synthetic Biology; Metabolic engineering

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1 and GAL10

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Linked VLP NES

   Plasmid

   #182432

   Purpose

   Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al bioRxiv 2022.11.24.517869

   Inserts

   VP2C-FPPS-NES
   deltaVP1
   

   Use

   Synthetic Biology; Metabolic engineering

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1 and GAL10

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Linked VLP NES (M)

   Plasmid

   #182434

   Purpose

   YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al bioRxiv 2022.11.24.517869

   Inserts

   VP2C-FPPS(M)-NES
   deltaVP1
   

   Use

   Synthetic Biology; Metabolic engineering

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1 and GAL10

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Linked VLP NES (G)

    Plasmid

    #182476

    Purpose

    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20(F96C)-AcNES1 (GAL10 promoter). Contains uracil marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    VP2C-FPPS(G)-NES
    deltaVP1
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 and GAL10

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAc5.1/V5-HisA-RvCAHS8-GS-AcGFP1

    Plasmid

    #192470

    Purpose

    Transient expression of RvCAHS8 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cells

    Depositor

    Takekazu Kunieda

    Article

    Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

    Insert

    CAHS8
    

    Use

    Tags

    AcGFP1

    Expression

    Insect

    Mutation

    Promoter

    Ac5 promoter

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAc5.1/V5-HisA-RvCAHS3-GS-AcGFP1

    Plasmid

    #192469

    Purpose

    Transient expression of RvCAHS3 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cells

    Depositor

    Takekazu Kunieda

    Article

    Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

    Insert

    CAHS3
    

    Use

    Tags

    AcGFP1

    Expression

    Insect

    Mutation

    Promoter

    Ac5 promoter

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEE044

    Plasmid

    #176801

    Purpose

    Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

    Insert

    413
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-4T-1-DGCR8-2xdsRBD(+6)

    Plasmid

    #171542

    Purpose

    Encodes a DGCR8 fragment extending from E502 to the C-terminus

    Depositor

    Yan Zeng

    Article

    Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

    Insert

    DGCR8 (DGCR8 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    a six amino acid_TRLTEG_insertion in the L1 region

    Promoter

    Available Since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSUMO FAPP2-GLTPH

    Plasmid

    #170739

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Ochoa-Lizarralde et al J Biol Chem. 2018 Oct 26;293(43):16709-1

    Insert

    PLEKHA8-GLTPH (PLEKHA8 Human)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. VP2C-GFP-MIOX only

    Plasmid

    #166684

    Purpose

    Yeast integrative plasmid for expressing mouse myo-inositol oxygenase fused to VP2C-GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Insert

    VP2C-GFP-MIOX
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. deltaVP1 only

    Plasmid

    #166675

    Purpose

    Yeast integrative plasmid for expressing deltaVP1, an NLS deletion mutant of Murine polyomavirus VP1 (GAL1 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Insert

    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCFJ2560 - pEXP(mosTI, unc-119)[Peft-3 | ceGFP(PATC, NLS) | tbb-2 3'UTR]

    Plasmid

    #159825

    Purpose

    Single copy or array insertion by MosTI using split unc-119 selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    pEXP(mosTI, unc-119)[Peft-3 | ceGFP(PATC, NLS) | tbb-2 3'UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRS314-U6-A34G/A35G/G96C/A97C/U100C/U101C

    Plasmid

    #81239

    Purpose

    U6 telestem mutant (A34G/A35G/G96C/A97C/U100C/U101C) for in vivo studies

    Depositor

    Aaron Hoskins

    Article

    Rodgers et al Nucleic Acids Res. 2016 Dec 15;44(22):10

    Insert

    U6 RNA with A34G/A35G/G96C/A97C/U100C/U101C mutation (snR6 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    U6 telestem mutant (A34G/A35G/G96C/A97C/U100C/U10…

    Promoter

    Available Since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-T7-AcrIIA1(CTD)-NLS(SV40) (KAC438)

    Plasmid

    #133796

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1 (truncated C-terminal domain only) with C-terminal NLS (SV40)

    Depositor

    Benjamin Kleinstiver, Joseph Bondy-Denomy

    Article

    Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

    Insert

    human codon optimized AcrIIA1 (truncated C-terminal domain only)
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    C-terminal domain only

    Promoter

    CMV and T7

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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