We narrowed to 8,596 results for: Set

1. pMVV102

   Plasmid

   #215308

   Purpose

   Expresses sfGFP under induction with the IPTG Marionette Cassette (LacIAM + PTac-sfGFP) with a BBR1 backbone.

   Depositor

   Brian Pfleger

   Article

   Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

   Insert

   sfGFP
   

   Use

   Synthetic Biology

   Tags

   None

   Expression

   Bacterial

   Promoter

   PTac

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMVV103

   Plasmid

   #215309

   Purpose

   Expresses sfGFP under induction with the aTc Marionette Cassette (TetR + PTet-sfGFP) with a BBR1 backbone.

   Depositor

   Brian Pfleger

   Article

   Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

   Insert

   sfGFP
   

   Use

   Synthetic Biology

   Tags

   None

   Expression

   Bacterial

   Promoter

   Ptet

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNBU2_erm-TetR-P1T_DP-GH023 - NanoLuc

   Plasmid

   #117728

   Purpose

   Integration vector at an attB site in Bacteroides; contains 1kb cassette of aTC-regulatable promoter (RBS GH023) upstream of the NanoLuc gene

   Depositor

   Andrew Goodman

   Article

   Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

   Insert

   NanoLuc
   

   Use

   Luciferase

   Expression

   Bacterial

   Available Since

   Dec. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. FRP1642_PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1

   Plasmid

   #58442

   Purpose

   Cassette to replace yeast promoters with an inducible promoter containing 4 lexA boxes

   Depositor

   Joerg Stelling

   Article

   Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

   Insert

   PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1
   

   Expression

   Yeast

   Promoter

   PTEF-HygMX-TTEF-insul-(lexA-box)4-PminCYC1

   Available Since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. 5UAS zfSynaptophysin:GFP-5UAS DsRedExpress

   Plasmid

   #74317

   Purpose

   A fusion of zebrafish synaptophysin to GFP and DsRedExpress under the control of separate UAS cassettes; for dual-labeling of retinal ganglion cell (RGC) axon arbors in zebrafish

   Depositor

   Martin Meyer, Stephen Smith

   Article

   Meyer et al J Neurosci. 2006 Mar 29;26(13):3604-14.

   Inserts

   synaptophysin
   DsRedExpress
   

   Tags

   EGFP

   Promoter

   5UAS

   Available Since

   May 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRTC2-puro

   Plasmid

   #190567

   Purpose

   Expresses the full-length human L1 (L1.3) retrotransposon and an engineered neomycin retrotransposition reporter to monitor retrotransposition efficiency in cultured mammalian cells.

   Depositor

   Anthony Furano

   Article

   Cook et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   L1 (L1.3) with reporter cassette
   

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPUR-hU6-Sirius-8XMS2-mU6-Sirius-8XPP7

   Plasmid

   #121944

   Purpose

   CRISPR-Sirius plasmid

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

   Insert

   hU6-Sirius-8XMS2-mU6-Sirius-8XPP7 Cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   no

   Expression

   Mammalian

   Promoter

   U6 promoters

   Available Since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNCS mRFP

   Plasmid

   #91765

   Purpose

   Bacterial expression of a fluorescent protein, monomeric Red Fluorescent Protein1 (mRFP1)

   Depositor

   Erik Rodriguez, Roger Tsien

   Article

   Campbell et al Proc Natl Acad Sci U S A. 2002 Jun 11;99

   Insert

   monomeric Red Fluorescent Protein1
   

   Tags

   Hexa-Histidine tag, Xpress epitope for detection …

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMC_mClover3_BSDminus_F2

   Plasmid

   #184167

   Purpose

   Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.

   Depositor

   Ophir Shalem

   Article

   Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

   Inserts

   mClover3
   bsd
   

   Use

   CRISPR

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMC_mClover3_BSDminus_F1

    Plasmid

    #184166

    Purpose

    Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants.

    Depositor

    Ophir Shalem

    Article

    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

    Inserts

    mClover3
    bsd
    

    Use

    CRISPR

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBBK28 pHomL-TDH3p-GFPdropout-Link-Neon(gfp)-SSA1t-HomR (AmpR)

    Plasmid

    #179012

    Purpose

    Parent vector for markerless yeast integration cassettes with C-terminal Neon(gfp)-tagged genes

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pL1P3 ZmUbiP:GRF-GIF:NosT

    Plasmid

    #198047

    Purpose

    MoClo Golden Gate Level 1 Position 3. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.

    Depositor

    Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing

    Insert

    ZmUbiP::TaGRF4-GIF1::NosT
    

    Use

    Synthetic Biology

    Promoter

    Zea mays (maize) ubiquitin promoter

    Available Since

    March 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEC3105 (pSpy1C-PxylS2_ffluc)

    Plasmid

    #218512

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporter

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Insert

    ffluc
    

    Use

    Luciferase

    Mutation

    deletion of Plac_mrfp cassette

    Promoter

    PxylS2

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAGC-Lb12-P1Bb

    Plasmid

    #231387

    Purpose

    For crRNA cloning with LbCas12a vectors for genome editing in Arabidopsis

    Depositor

    Qi-Jun Chen

    Article

    Xin et al aBIOTECH. 2024 Mar 26;5(2):117-126. doi:

    Insert

    U6-29
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMVV109

    Plasmid

    #215339

    Purpose

    Expresses sfGFP under induction with the 3OC14-HSL Marionette Cassette (CinRAM + PCin-sfGFP) with a BBR1 backbone.

    Depositor

    Brian Pfleger

    Article

    Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Promoter

    PCin

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. PAX6 donor plasmid

    Plasmid

    #105239

    Purpose

    Knock in H2B-GFP into the human PAX6 locus

    Depositor

    Lorenz Studer

    Article

    Tchieu et al Cell Stem Cell. 2017 Sep 7;21(3):399-410

    Insert

    PAX6 Homologous recombination ARMS with a P2A-H2B-GFP and Puromycin selection cassette
    

    Use

    TALEN

    Available Since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFA6a-GFP(S65T)-kanMX6

    Plasmid

    #39292

    Depositor

    Jurg Bahler, John Pringle

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    KanR
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Available Since

    Aug. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOCC151

    Plasmid

    #118899

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal GST, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-GST-3C-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    GST tag, cleavable with 3C protease

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCP-tRNA

    Plasmid

    #133812

    Purpose

    CRISPR-Cas9 system for genetic manipulation of Candida parapsilosis, C. orthopsilosis, and C. metapsilosis

    Depositor

    Geraldine Butler

    Article

    Lombardi et al mSphere. 2019 Mar 13;4(2). pii: 4/2/e001

    Insert

    cassette for the expression of the sgRNA from the C. parapsilosis RNA pol II GAPDH promoter
    

    Use

    CRISPR

    Available Since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. Halo-53BP1 HRD

    Plasmid

    #207087

    Purpose

    Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous 53BP1 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    HaloTag with internal PuroR cassette flanked by human 53BP1 locus sequences
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    Endogenous

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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