We narrowed to 10,197 results for: otos
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Plasmid#224495PurposeGateway compatible middle entry clone with Actin nanobody fused to sfGFP (GFP actin reporter)DepositorInsertActin-Vhh-sfGFP
UseGateway cloningAvailable SinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME_Golgi-BFP_P2A_H2A_iRFP (JDW 1007)
Plasmid#224490PurposeGateway compatible middle entry clone containing GalT-mTagBFP-HA-P2A-H2A-iRFP (BFP golgi reporter and infrared RFP nuclear reporter)DepositorInsertGolgi-mTagBFP-P2A-H2A-iRFP
UseGateway cloningAvailable SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-flex-GFP-shRNA-GluN1
Plasmid#182502PurposeExpresses shRNA against mouse NMDA receptor NR1 subunit. Flexed cassette driven by hdc (pan neuronal) gene promoterDepositorInsertpan neuronal promoter (hdc basal promoter) driving flexed-GFP-mir30-shRNA NR1 cassette
UseAAV, Cre/Lox, and RNAiExpressionMammalianPromoterfragment of histidine decarboxylase gene promoter…Available SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
HA-YAP-wt
Plasmid#229412PurposeLentiviral or overpexression of cDNADepositorAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK092
Plasmid#183146PurposepET-6xHis-MBP-TEV-CrtTIR-APAZ/CrtAgo - Heterologous CrtSPARTA expressionDepositorInsertCrtSPARTA operon (6xHis-MBP-CrtTIR-APAZ and CrtAgo)
Tags6xHis and MBPExpressionBacterialPromoterT7Available SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBK086
Plasmid#183145PurposepET-6xHis-MBP-TEV-MapTIR-APAZ/MapAgo - Heterolgous MapSPARTA expressionDepositorInsertMapSPARTA operon (6xHis-MBP-MapTIR-APAZ and MapAgo)
Tags6xHis and MBPExpressionBacterialPromoterT7Available SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-MYO10-HMM
Plasmid#87256PurposeExpression plasmid to perform NanoSPD assays in mammalian cells (generates EGFP-tagged bait).DepositorInsertMYO10 (Myo10 Mouse)
ExpressionMammalianMutationMyo10 is truncated to contain aa.1-941PromoterCMVAvailable SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS0045
Plasmid#131124PurposeMammalian Target-ACE expression vectorDepositorInsertTadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nCas9-PmCDA1-UGI
UseCRISPRTagsPmCDA1, TadA, and UGIExpressionMammalianMutationA262T, R324L, S409I, E480K, E543D, M694I, E1219V …PromoterCMV promoterAvailable SinceSept. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYI2165
Plasmid#228347Purpose2,4-Diacetylphloroglucinol (DAPG)-regulatable ALX-0171 expressionDepositorInsertMFalpha(2xAdv)-LE-ALX-0171-3A-FLAG-His
UseAffinity Reagent/ AntibodyTagsAAA linker followed by FLAG-His6 and MF_ secretio…ExpressionYeastPromoter2,4-Diacetylphloroglucinol-regulatable synthetic …Available SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
OMM-Flag-APEX2-SOPP3
Plasmid#229073PurposeExpresses APEX2-SOPP3 targeted to outer mitochondrial membraneDepositorInsertExpress APEX2-SOPP3 in outer mitochondrial membrane (APEX2 Human)
ExpressionMammalianPromoterCMVAvailable SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-human full-length LIC1
Plasmid#74597Purposeused for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tagDepositorInsertfull length human dynein light intermediate chain 1 (DYNC1LI1 Human)
TagsN-terminal GST and Strep IIExpressionBacterialPromoterT7Available SinceMay 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMRXIB-mRuby3-CERT(PHD)
Plasmid#221762PurposeStable expression of mRuby3-CERT(PHD) in mammalian cellsDepositorInsertCERT (CERT1 Human)
UseRetroviralTagsmRuby3ExpressionMammalianMutationamino acids 1–116Available SinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME Actin Vhh mNeonGreen-HA-pA (JDW 1248)
Plasmid#224500PurposeGateway compatible middle entry clone containing Actin nanobody fused to mNeonGreen with an HA tag (Visualizing actin, actin chromobody)DepositorInsertActin-Vhh-mNeonGreen-HA-pA
UseGateway cloningAvailable SinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCOTS-pyl-GFP(35TAG)
Plasmid#92047PurposeThis is a S. elongatus (PCC7942) cyanobacterial plasmid that encodes the pylRS orthogonal translation system. In addition it encodes for a GFP reporter with TAG mutation at site 35.DepositorInsertsEGFP
Pyrrolysyl tRNA(cua)
Pyrrolysyl tRNA synthetase (methanosarcina mazei)
UseReplicative expression plasmid for cyanobacteria …TagsHistagMutationTyrosine in position 35 of the GFP was mutated f…PromoterLeuP (native S. elongatus promoter), PpsbII, and …Available SinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-REX-GECO1
Plasmid#61248PurposeExpresses REX-GECO1 in neuronsDepositorInsertREX-GECO1
UseAAVExpressionMammalianMutationSubstitutions relative to R-GECO1: P60R, V61W, R6…PromoterhSynAvailable SinceMarch 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-eEF2K(S441A/S445A)
Plasmid#110161PurposeExpression of human eEF2K (S441A/S445A) in mammalian cellsDepositorInserteEF2K (eukaryotic elongation factor 2 kinase) (EEF2K Human)
TagsHAExpressionMammalianMutationS441A/S445APromoterCMVAvailable SinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFIN-RK-GFP-WPRE
Plasmid#44358DepositorInsertRK-GFP (MAPK14 Synthetic, Human)
UseLentiviralPromoterhuman rhodopsin kinase (pos 2557-2853)Available SinceMay 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHX_HDR_hPAX7_IRES-EGFP-PGK-Neo
Plasmid#160458PurposeHomology-directed recombination donor vector for EGFP reporter knock-in to human PAX7 3' UTRDepositorUseInsertion of a fluorescent reporter through homol…Available SinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF-DEST51-GOLPH3
Plasmid#21690DepositorInsertGolgi Phosphoprotein 3 (GOLPH3 Human)
UseInvitrogen gateway destination vectorTags6xHIS and V5ExpressionMammalianAvailable SinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLEX-rc[Chronos-GFP]
Plasmid#59056PurposeExpression vector for Chronos-GFP in a floxed/reversed, Cre-dependent manner (Cre turns gene on)DepositorInsertChronos-GFP
TagsGFPExpressionMammalianPromoterCAGAvailable SinceAug. 13, 2014AvailabilityAcademic Institutions and Nonprofits only