We narrowed to 3,042 results for: NAK

1. pDonor-tBFP-NLS-Neo

   Plasmid

   #80766

   Purpose

   Customizable donor vector for CRISPR/Cas9-mediated homology-independent knock-in system.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   TagBFP2-3×NLS

   Expression

   Mammalian

   Available Since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX6P1-mClover3-anti-rabbit-IgG(Fc)-nanobody

   Plasmid

   #138129

   Purpose

   Expresses GST-tagged mClover3-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

   Insert

   mClover3-anti-rabbit-IgG(Fc)-nanobody
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMy-BirA-T2A-eGFP

   Plasmid

   #50565

   Purpose

   Retroviral vector encoding for GFP and the biotinylating enzyme BirA

   Depositor

   Rafael Casellas

   Article

   Nakahashi et al Cell Rep. 2013 May 30;3(5):1678-89. doi:

   Insert

   Ampicilin
   

   Use

   Retroviral

   Available Since

   Feb. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-C1-hArfaptin2

   Plasmid

   #206405

   Purpose

   Mammalian expression of hArfaptin2

   Depositor

   Hye-Won Shin, Kazuhisa Nakayama

   Article

   Shin et al J Cell Sci. 2005 Sep 1;118(Pt 17):4039-4

   Insert

   Arfaptin2 (ARFIP2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Oct. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-hARFGAP1-HA

   Plasmid

   #206409

   Purpose

   Mammalian expression of hARFGAP1

   Depositor

   Hye-Won Shin, Kazuhisa Nakayama

   Article

   Saitoh et al J Biol Chem. 2009 May 15;284(20):13948-1

   Insert

   ARFGAP1 (ARFGAP1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. ORAI1-hBACCS2

   Plasmid

   #67626

   Purpose

   Mammalian expression of ORAI1-hBACCS2, a fusion protein of ORAI1 and human blue light-activated Ca2+ channel switch

   Depositor

   Takao Nakata

   Article

   Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

   Insert

   ORAI1-hBACCS2
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMXs-Klf4-IP

   Plasmid

   #15920

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

   Insert

   Kruppel-like factor 4 (gut) (Klf4 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Feb. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXs-Sox2-IP

   Plasmid

   #15919

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

   Insert

   SRY-box containing gene 2 (Sox2 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Feb. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. DCX-RFP

   Plasmid

   #32851

   Depositor

   Joseph Gleeson

   Article

   Tanaka et al J Cell Biol. 2004 Jun 7. 165(5):709-21.

   Insert

   DCX (DCX Human)
   

   Tags

   DsRed2

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Nov. 15, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pCAP01

    Plasmid

    #59981

    Purpose

    The function of this material is to facilitate the capture and expression of bacterial gene clusters.

    Depositor

    Bradley Moore

    Article

    Yamanaka et al Proc Natl Acad Sci U S A. 2014 Feb 4;111

    Type

    Empty backbone

    Use

    Synthetic Biology; Transformation-associated reco…

    Tags

    no

    Expression

    Bacterial and Yeast

    Promoter

    no

    Available Since

    Nov. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMXS-hs-3xHA-LIN-41

    Plasmid

    #52717

    Purpose

    retroviral expression of human LIN-41 / TRIM71 with 3 N-terminal HA tags

    Depositor

    Shinya Yamanaka

    Article

    Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

    Insert

    LIN-41 (TRIM71 Human)
    

    Use

    Retroviral

    Tags

    3xHA

    Expression

    Mammalian

    Available Since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMXs-hs-LIN-41

    Plasmid

    #52716

    Purpose

    retroviral expression of human LIN-41 / TRIM71

    Depositor

    Shinya Yamanaka

    Article

    Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

    Insert

    LIN-41 (TRIM71 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. YFP-SspBx6

    Plasmid

    #103778

    Purpose

    expression of iPOLYMER component in mammalian cells

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

    Insert

    YFP-SspBx6
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3/hArf5(WT)-HA

    Plasmid

    #79427

    Purpose

    Expresses C-terminally HA-tagged Arf5(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Hanai et al J Biochem. 2016 Feb;159(2):201-8. doi: 1

    Insert

    Arf5 (ARF5 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSN838

    Plasmid

    #184826

    Purpose

    Expresses human KIF5A(∆exon27) in mammalian cells

    Depositor

    Kyoko Chiba

    Article

    Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g

    Insert

    KIF5A (∆exon27) (KIF5A Human)
    

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    ∆exon27 form

    Promoter

    CMV

    Available Since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-4T-2/hGGA3(GAT)

    Plasmid

    #79436

    Purpose

    Expresses GST-tagged GAT domain of GGA3 (107-286 aa) in E.coli

    Depositor

    Kazuhisa Nakayama

    Article

    Takatsu et al Biochem J. 2002 Jul 15;365(Pt 2):369-78.

    Insert

    GGA3 (GGA3 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    amino acids 107-286

    Available Since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMy-CTCF-GFP

    Plasmid

    #50574

    Purpose

    Retroviral vector endoding for mouse CTCF fused to GFP

    Depositor

    Rafael Casellas

    Article

    Nakahashi et al Cell Rep. 2013 May 30;3(5):1678-89. doi:

    Insert

    Ampicilin
    

    Use

    Retroviral

    Available Since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. dmBACCS2-IRES-dOrai

    Plasmid

    #67627

    Purpose

    Mammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, and dOrai

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2-IRES-dOrai
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. CAG-lox-CAT-lox-unique Bam cloning site-hGH polyA (Clone 17)

    Plasmid

    #53959

    Purpose

    allows Cre-mediated expression of transgene

    Depositor

    Jeffrey Robbins

    Article

    Nakamura et al Proc Natl Acad Sci U S A. 2009 Sep 8;106

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Lox-CAT-Lox

    Expression

    Mammalian

    Promoter

    CMV enhancer, chicken actin promoter

    Available Since

    June 30, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pAH243

    Plasmid

    #121147

    Purpose

    Expression of both nmt41p-cas9 and rrk1p-gRNA (ura4 marker) for CRISPR genome editing in fission yeast

    Depositor

    Katsunori Tanaka

    Article

    Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

    Inserts

    humanized Streptococcus pyogenes Cas9
    gRNA expression module
    

    Use

    CRISPR

    Tags

    Flag

    Expression

    Yeast

    Promoter

    S.pombe rrk1 and nmt41

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. CFP-FRBx5-His

    Plasmid

    #103784

    Purpose

    expresses His-tagged iPOLYMER component in E. coli for protein purification

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

    Insert

    CFP-FRBx5-His
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. YFP-FKBPx5

    Plasmid

    #103775

    Purpose

    expresses iPOLYMER component in mammalian cells

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

    Insert

    YFP-FKBPx5
    

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. YFP-FKBPx5-His

    Plasmid

    #103785

    Purpose

    expresses His-tagged iPOLYMER component in E. coli for protein purification

    Depositor

    Takanari Inoue

    Article

    Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

    Insert

    YFP-FKBPx5-His
    

    Tags

    EYFP, His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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24. POLArIS_NbVim-cp10GFP

    Plasmid

    #171020

    Purpose

    Expresses NbVim-cp10GFP#2 in mammalian cells

    Depositor

    Sumio Terada

    Article

    Nakai et al Biochem Biophys Res Commun. 2021 Jun 2;5

    Insert

    anti-vimentin-nanobody VB6 (NbVim) tagged with circularly permutated superfolderGFP(cp10GFP)
    

    Tags

    cp10GFP

    Expression

    Bacterial and Mammalian

    Promoter

    CMV promoter

    Available Since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pG23A

    Plasmid

    #102884

    Purpose

    Plasmid used for the C-terminal tagging of Cdc14 with mCherry and the auxin-inducible degron in yeast.

    Depositor

    Matthias Heinemann

    Article

    Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

    Insert

    Cdc14/mCherry-AID(71-114)-NatMX (CDC14 Budding Yeast)
    

    Tags

    AID(71-114) and mCherry

    Expression

    Yeast

    Promoter

    Native promoter

    Available Since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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26. pRvCAHS3-mEGFP

    Plasmid

    #205008

    Purpose

    The vector contains 1kbp of the upstream and downstream regions of the cahs3 gene from Ramazzottius varieornatus, with the mEGFP gene positioned in between.

    Depositor

    Kazuharu Arakawa

    Article

    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

    Insert

    mEGFP flanked by 1kbp upstream and downstream of the cahs3 gene from Ramazzottius varieornatus
    

    Use

    Tardigrade expression

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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27. pRvACT-mCherry

    Plasmid

    #205002

    Purpose

    The vector contains 1kbp of the upstream and downstream regions of the actin gene from Ramazzottius varieornatus, with the mCherry gene with NLS sequence positioned in between.

    Depositor

    Kazuharu Arakawa

    Article

    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

    Insert

    mCherry-NLS flanked by 1kbp upstream and downstream of the actin gene from R. varieornatus
    

    Use

    Tardigrade expression

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGEX-4T-2/hGGA1(GAT)

    Plasmid

    #79435

    Purpose

    Expresses GST-tagged GAT domain of GGA1 (141-327 aa) in E.coli

    Depositor

    Kazuhisa Nakayama

    Article

    Takatsu et al Biochem J. 2002 Jul 15;365(Pt 2):369-78.

    Insert

    GGA1 (GGA1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    amino acids 141-327

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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29. pRVdG-4RFP

    Plasmid

    #52489

    Purpose

    Expresses TagRFP-T

    Depositor

    Sebastian Seung

    Article

    Nakashiba et al Cell. 2012 Mar 30;149(1):188-201. doi: 1

    Insert

    TagRFP-T
    

    Use

    Rabies virus

    Promoter

    CMV

    Available Since

    Aug. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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30. pcDNA3 WT MYOC eGLuc2 FLAG

    Plasmid

    #192820

    Purpose

    Expresses wild-type myocilin with a C-terminal enhanced Gaussia luciferase and FLAG tag

    Depositor

    John Hulleman

    Article

    Nakahara et al Curr Eye Res. 2022 Jun;47(6):918-922. do

    Insert

    myocilin (MYOC Human)
    

    Tags

    FLAG and enhanced Gaussia luciferase (M43I/M110I)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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