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Addgene

We narrowed to 1,159 results for: smo

Showing: 931 - 960 of 1159 results
  1. iCyt_F_SnFR

    Plasmid
    #177739
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Insert
    iCyt_F_SnFR
    Use
    Tags
    HA, His, and Myc
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. pCDNA3_PKA-Cα(S11D)-mPAGFP

    Plasmid
    #114135
    Purpose
    Mammalian expression of the S11D mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.
    Depositor
    Insert
    the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with S11D mutation (Prkaca Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
  3. pCAGGS_m-AKAR5

    Plasmid
    #114139
    Purpose
    Mammalian expression of the AKAR5 sensor C-terminally fused to the C-terminus of KRas (membrane targeting) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)
    Depositor
    Insert
    AKAR5 C-terminally tagged by KRas C tail
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
  4. pCAGGS_cyt-AKAR5

    Plasmid
    #114138
    Purpose
    Mammalian expression of the AKAR5 sensor tagged with nucleus export signal (NES) for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)
    Depositor
    Insert
    AKAR5 with C-terminal nuclear export signal (NES)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
  5. pCDNA3_PKA-Cα(S11A)-mPAGFP

    Plasmid
    #114134
    Purpose
    Mammalian expression of the S11A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.
    Depositor
    Insert
    the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with S11A mutation (Prkaca Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
  6. pCDNA3_PKA-CαN-mPAGFP

    Plasmid
    #114114
    Purpose
    Mammalian expression of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.
    Depositor
    Insert
    the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP (Prkaca Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
  7. pCDNA3-EMTB-TurboID-V5

    Plasmid
    #190736
    Purpose
    Mammalian expression of EMTB fusion to V5-tagged TurboID on microtubules (EMTB as the microtubule-targeting tag)
    Depositor
    Insert
    ensconsin (MAP7 Human)
    Use
    Tags
    Myc, TurboID, and V5
    Expression
    Mammalian
    Mutation
    Microtubule binding-domain of ensconsin (amino ac…
    Promoter
    CMV
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
  8. pCDH-DCX-TurboID-V5-puro

    Plasmid
    #190742
    Purpose
    A lentiviral plasmid encoding DCX fusion to V5-tagged TurboID on microtubules (DCX as the microtubule-targeting tag)
    Depositor
    Insert
    DCX-TurboID-V5 (DCX Human, DCX is human doublecortin; TurboID is engineered BirA from E.coli)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
  9. pDiCre-mCherry

    Plasmid
    #164572
    Purpose
    Integration of a DiCre cassette in PbGFP parasite genome
    Depositor
    Inserts
    FKBP12-CRE59
    FRB-CRE60
    mCherry
    Bifunctional dihydrofolate reductase-thymidylate synthase (TGME49_249180 Toxoplasma gondii)
    Use
    Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Bidirectional PbEF1alpha promoter (PBANKA_1133300…
    Available since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 931 - 960 of 1159 results