We narrowed to 2,493 results for: ERK

1. pCrepusculo

   Plasmid

   #190156

   Purpose

   Regulates bacterial expression of target gene in blue-light-repressed manner. Based on Nakamurella multipartita PAL.

   Depositor

   Andreas Moeglich

   Article

   Ranzani et al ACS Synth Biol. 2022 Oct 21;11(10):3482-

   Type

   Empty backbone

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMSCV MEKK3 PB1

   Plasmid

   #12185

   Depositor

   Gary Johnson

   Article

   Nakamura et al J Biol Chem. 2003 Sep 26. 278(39):36989-

   Insert

   MEKK3 (Map3k3 Mouse)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   PB1 domain: aa 36-127

   Promoter

   Available since

   Sept. 22, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pMH4

   Plasmid

   #52529

   Purpose

   serves as PCR-template to generate HR donors for C-terminal 2xFLAG-tag

   Depositor

   Klaus Foerstemann

   Article

   Bottcher et al Nucleic Acids Res. 2014 Apr 19.

   Insert

   2x FLAG-tag
   

   Use

   Blunt cloning kit

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. tdKatushka2-VSVG-16

   Plasmid

   #56052

   Purpose

   Localization: Golgi/ER Transport, Excitation: 586, Emission: 632

   Depositor

   Michael Davidson

   Article

   Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

   Insert

   VSVG
   

   Use

   Tags

   tdKatushka2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 1, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

5. HS-Utrophin-GFP

   Plasmid

   #105952

   Purpose

   heat shock inducible transgenesis

   Depositor

   Caren Norden

   Article

   Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

   Insert

   Utrophin (Utrn Mouse)
   

   Use

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCin4-3xFLAG-MKL1 S454A

   Plasmid

   #19844

   Depositor

   Ron Prywes

   Article

   Muehlich et al Mol Cell Biol. 2008 Aug 11. ():.

   Insert

   MKL1 S454A (MRTFA Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   changed Serine 454 to Alanine

   Promoter

   Available since

   Dec. 16, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pFA-LEU2-I-SceI

   Plasmid

   #67595

   Purpose

   I-SceI expression regulated by tetracycline inducible system in S. pombe

   Depositor

   Kurt Runge

   Article

   Wang et al Mol Cell Biol. 2018 Jul 16;38(15). pii:

   Inserts

   nmt terminator
   I-SceI
   TetR
   

   Use

   Gene targeting in yeast

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. HS-DN-Plk1

   Plasmid

   #105949

   Purpose

   heat shock inducible transgenesis

   Depositor

   Caren Norden

   Article

   Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

   Insert

   Plk1 (PLK1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Dominant negative K82 R

   Promoter

   Available since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRSFDuet1_CoiA1_CoiB

   Plasmid

   #208761

   Purpose

   Precursor peptide CoiA1 and synthase CoiB, for the co-expression of CoiA1 in E.coli.

   Depositor

   Wilfred van der Donk

   Article

   Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

   Inserts

   CoiA1
   CoiB
   

   Use

   Tags

   Hisx6 Tag and SUMO

   Expression

   Bacterial

   Mutation

   Changed Ser 20 to Asn, Changed Arg 84 to Lys, bot…

   Promoter

   T7

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAc5.1B-EGFP-DmGW182

    Plasmid

    #22419

    Depositor

    Elisa Izaurralde

    Article

    Behm-Ansmant et al Genes Dev. 2006 Jul 15. 20(14):1885-98.

    Insert

    gawky, variant A (gw Fly)
    

    Use

    Drosophila expression

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAc5.1B-lambdaN-HA-DmStaufen_B

    Plasmid

    #145947

    Purpose

    Insect Expression of DmStaufen

    Depositor

    Elisa Izaurralde

    Article

    Behm-Ansmant et al Genes Dev. 2006 Jul 15. 20(14):1885-98.

    Insert

    DmStaufen (stau Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pXWJ109As-gfp

    Plasmid

    #123143

    Purpose

    An arsenic sensor with optimized intracellular receptor density. pSB3K3 carrying J109-30arsR-t-ParsR-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-30arsR-t-ParsR-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J109, ParsR

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAc5.1B-lambdaN-HA-DmGW182

    Plasmid

    #22406

    Depositor

    Elisa Izaurralde

    Article

    Behm-Ansmant et al Genes Dev. 2006 Jul 15. 20(14):1885-98.

    Insert

    gawky, variant A (gw Fly)
    

    Use

    Drosophila expression

    Tags

    lambdaN-HA

    Expression

    Mutation

    Promoter

    Available since

    Nov. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pIW1

    Plasmid

    #52530

    Purpose

    serves as PCR-template to generate HR donors for C-terminal Twin-Strep tag

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    Twin-STREP-tag
    

    Use

    Blunt cloning kit

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHR-MCS-YFP-NLS

    Plasmid

    #145274

    Purpose

    Mammalian Expression of Nuclear YFP with N-terminal MCS and SV40 NLS

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    MCS and YFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p3xFLAG-MKL1 D301-380

    Plasmid

    #19852

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Aug 11. ():.

    Insert

    MKL1 (MRTFA Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    deleted amino acids 301 to 380

    Promoter

    Available since

    Dec. 16, 2008

    Availability

    Academic Institutions and Nonprofits only

17. pRB20

    Plasmid

    #52559

    Purpose

    serves as PCR-template to generate HR donors for C-terminal YFP-tag

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    eYFP and Blasticigin resistance cassette
    

    Use

    Blunt cloning kit

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCin4-3xFLAG-MKL1 T450A

    Plasmid

    #19843

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Aug 11. ():.

    Insert

    MKL1 T450A (MRTFA Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed Threonine 450 to Alanine

    Promoter

    Available since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

19. p3xFLAG-MKL1 D381-506

    Plasmid

    #19853

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Aug 11. ():.

    Insert

    MKL1 (MRTFA Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    deleted amino acids 381 to 506

    Promoter

    Available since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

20. 4E2H.210_LUMABS

    Plasmid

    #155209

    Purpose

    Expresses 4E2H.210_LUMABS sensor in E.coli

    Depositor

    Bruno Correia

    Article

    Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

    Insert

    4E2H.210_LUMABS
    

    Use

    Tags

    6x His Tag

    Expression

    Mutation

    Promoter

    Available since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. tdKatushka2-CytERM-N-17

    Plasmid

    #56037

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 586, Emission: 632

    Depositor

    Michael Davidson

    Article

    Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

    Insert

    CytERM
    

    Use

    Tags

    tdKatushka2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

22. psecBFP2

    Plasmid

    #62230

    Purpose

    encodes an inert blue fluorescent protein for use in oxidizing cellular environments including the periplasm and mammalian secretory pathway.

    Depositor

    Erik Snapp

    Article

    Costantini et al Biochem Biophys Res Commun. 2013 Jan 18;

    Insert

    secBFP2
    

    Use

    Tags

    secbfp2

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

23. As5 (pBW414J105-arsR)

    Plasmid

    #123162

    Purpose

    Arsenic sensor used in the sensor cell array. pSB3K3 carrying J105-30arsR-t-ParsR-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J105-30arsR-t-ParsR-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J105, ParsR

    Available since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCin4-3xFLAG-MKL1 L272A

    Plasmid

    #19850

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Aug 11. ():.

    Insert

    MKL1 L272A (MRTFA Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed Leucine 272 to Alanine

    Promoter

    Available since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

25. pMSCV MEKK2 PB1

    Plasmid

    #12184

    Depositor

    Gary Johnson

    Article

    Nakamura et al J Biol Chem. 2003 Sep 26. 278(39):36989-

    Insert

    MEKK2 (Map3k2 Mouse)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    PB1 domain: aa 35-126

    Promoter

    Available since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

26. pKF251

    Plasmid

    #52532

    Purpose

    Double-GFP reporter for DNA cleavage

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    Double-GFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    ubiquitin

    Available since

    April 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCin4-3xFLAG-MKL1 S449A

    Plasmid

    #19842

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Aug 11. ():.

    Insert

    MKL1 S449A (MRTFA Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed Serine 449 to Alanine

    Promoter

    Available since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

28. mKate1.31-CytERM-N-17

    Plasmid

    #56080

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 588, Emission: 635

    Depositor

    Michael Davidson

    Article

    Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

    Insert

    CytERM
    

    Use

    Tags

    mKate1.31

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 13, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pAc5.1A-Dm-miR-9b

    Plasmid

    #22710

    Depositor

    Elisa Izaurralde

    Article

    Eulalio et al Genes Dev. 2007 Oct 15. 21(20):2558-70.

    Insert

    micro RNA 9b (mir-9b Fly, primary transcript)
    

    Use

    Drosophila expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAc5.1A-Dm-miR-92b

    Plasmid

    #22709

    Depositor

    Elisa Izaurralde

    Article

    Eulalio et al Genes Dev. 2007 Oct 15. 21(20):2558-70.

    Insert

    micro-RNA-92b (mir-92b Fly, primary transcript)
    

    Use

    Drosophila expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. B8-pVTCB052

    Plasmid

    #231302

    Purpose

    Plasmid used for expression of AI-designed GFP protein B8 in E. coli

    Depositor

    Alexander Rives

    Insert

    B8
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. YIplac204-TDH3p-CDC19-CYC1t

    Plasmid

    #216122

    Purpose

    Yeast integrative vector for constitutive expression of CDC19 at the TRP1 locus

    Depositor

    Yvonne Nygård

    Article

    Choi et al Metab Eng. 2024 Jul;84:23-33. doi: 10.10

    Insert

    Pyruvate kinase 1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3 promoter

    Available since

    Dec. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pPI161

    Plasmid

    #207439

    Purpose

    Shuttle vector LifeAct-mCherry

    Depositor

    Rob Kay

    Article

    Nichols et al Dev Cell. 2019 Feb 25;48(4):491-505.e9.

    Insert

    LifeAct-mCherry
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. GFP hSmad4-HomDonor

    Plasmid

    #206044

    Purpose

    Donor plasmid for CRISPR knock-in

    Depositor

    Ali Hemmati Brivanlou

    Article

    Nemashkalo et al Development. 2017 Sep 1;144(17):3042-305

    Insert

    Smad4 (SMAD4 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET_4E1H.95

    Plasmid

    #155213

    Purpose

    Expresses 4E1H.95 de novo protein in E.coli

    Depositor

    Bruno Correia

    Article

    Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

    Insert

    4E1H.95
    

    Use

    Tags

    6x His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET_4H.01

    Plasmid

    #155210

    Purpose

    Expresses 4H.01 de novo designed protein in E.coli

    Depositor

    Bruno Correia

    Article

    Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

    Insert

    4H.01
    

    Use

    Tags

    6x His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. 4E1H.95_LUMABS

    Plasmid

    #155208

    Purpose

    Expresses 4E1H.95_LUMABS sensor in E.coli

    Depositor

    Bruno Correia

    Article

    Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

    Insert

    4E1H.95_LUMABS
    

    Use

    Tags

    6x His Tag

    Expression

    Mutation

    Promoter

    Available since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET_3E2H.37

    Plasmid

    #155211

    Purpose

    Expresses 3E2H.37 de novo designed protein in E.coli

    Depositor

    Bruno Correia

    Article

    Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

    Insert

    3E2H.37
    

    Use

    Tags

    6x His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET_3H1L_02.395

    Plasmid

    #155198

    Purpose

    Expresses 3H1L_02.395 in E.coli

    Depositor

    Bruno Correia

    Article

    Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

    Insert

    3H1L_02.395
    

    Use

    Tags

    6x His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. p35S::AtSOBIR1-YFPc

    Plasmid

    #86162

    Purpose

    SOBIR1 split YFP

    Depositor

    Silke Robatzek

    Article

    Postma et al New Phytol. 2016 Apr;210(2):627-42. doi:

    Insert

    SOBIR1
    

    Use

    Tags

    YFPc

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge