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Showing: 1551 - 1600 of 2791 results

  2. pHL 100

    Plasmid
    #52989
    Purpose
    pLlac0-1:OxyS-mCherry, pLtetO-1:fhlA::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    OxyS sRNA
    fhlA
    Use
    Synthetic Biology
    Tags
    GFP and RBS-mCherry
    Expression
    Bacterial
    Mutation
    −309 to +96 bp relative to start codon
    Promoter
    pLlac0-1 and pLtetO-1
    Available since
    Jan. 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  3. pHL 802

    Plasmid
    #52991
    Purpose
    pLtetO-1:OxyS-mCherry, pLlacO-1:ompC::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    OxyS sRNA
    ompC
    Use
    Synthetic Biology
    Tags
    GFP and RBS-mCherry
    Expression
    Bacterial
    Mutation
    contains −81 to +36 bp relative to start codon
    Promoter
    pLlacO-1 and pLtetO-1
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  4. pHL 1148

    Plasmid
    #53006
    Purpose
    pCon:OxyS, pLlacO-1:sodB::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    OxyS sRNA
    sodB
    Use
    Synthetic Biology
    Tags
    GFP
    Expression
    Bacterial
    Mutation
    contains −56 to +141 bp relative to start codon
    Promoter
    pCon and pLlacO-1
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  5. pHL 1070

    Plasmid
    #53002
    Purpose
    pCon:DsrA, pLlacO-1:sodB::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    DsrA sRNA
    sodB
    Use
    Synthetic Biology
    Tags
    GFP
    Expression
    Bacterial
    Mutation
    contains −56 to +141 bp relative to start codon
    Promoter
    pCon and pLlacO-1
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  6. pHL 1057

    Plasmid
    #53001
    Purpose
    pCon:RhyB, pLlacO-1:rpoS::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    RhyB sRNA
    rpoS
    Use
    Synthetic Biology
    Tags
    GFP
    Expression
    Bacterial
    Mutation
    containsn −150 to +30 bp relative to start codon
    Promoter
    pCon and pLlacO-1
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  8. CMV-Hylight

    Plasmid
    #193447
    Purpose
    A fluorescent biosensor for fructose 1,6-bisphosphate (FBP). Changes in FBP concentration strongly correlate with changes in glycolytic flux. (mammalian expression vector)
    Depositor
    Richard Goodman
    Article
    Koberstein et al Proc Natl Acad Sci U S A. 2022 Aug 2;119
    Insert
    Hylight Biosensor
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    193447-plasmid-map-sequence-id-378450

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  14. piRFP

    Plasmid
    #31857
    Purpose
    a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging
    Depositor
    Vladislav Verkhusha
    Article
    Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103
    Has Service
    Cloning Grade DNA
    Insert
    iRFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…
    Promoter
    CMV
    Available since
    Sept. 6, 2011
    Availability
    Academic Institutions and Nonprofits only
    31857-plasmid-map-sequence-id-396432

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  17. Lenti-UbC-FKRP-EF1a-BSD

    Plasmid
    #205150
    Purpose
    Express UbC-driven FKRP and EF-1a driven BSD
    Depositor
    Monkol Lek
    Insert
    Fukutin-related protein (FKRP Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    UbC
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    205150-plasmid-map-sequence-id-405993

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  19. pLV-mRFP1-Human UTROPHIN_1-261

    Plasmid
    #225052
    Purpose
    Express in mammalian cells the mRFP1 fused to residues 1-261 of Human Utrophin to detect filamentous actin in live, fix cels or tissues.
    Depositor
    Pantelis Tsoulfas
    Insert
    Human Utrophin residues 1-261 and RFP1 (UTRN Human, Homo Sapiens)
    Use
    Lentiviral
    Tags
    Human UTROPHIN 1-261 and mRFP1
    Expression
    Mammalian
    Mutation
    Sequence is derived from ADDGENE Plasmid #26739-R…
    Promoter
    CMV promoter and enhancer
    Available since
    Sept. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    225052-plasmid-map-sequence-id-446521

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  20. pLV-EGFP-Human-UTROPHIN_1-261

    Plasmid
    #222393
    Purpose
    Express in mammalian cells the EGFP fused to residues 1-261 of Human Utrophin to detect filamentous actin in live, fix cels or tissues.
    Depositor
    Pantelis Tsoulfas
    Insert
    Human Utrophin residues 1-261 and EGFP (UTRN Human, Homo Sapiens)
    Use
    Lentiviral
    Tags
    EGFP and Human UTROPHIN 1-261
    Expression
    Mammalian
    Mutation
    Sequence is derived from ADDGENE Plasmid #26737-G…
    Promoter
    CMV promoter and enhancer
    Available since
    Sept. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    222393-plasmid-map-sequence-id-446518

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  23. pSG5-ERRbeta2

    Plasmid
    #52186
    Purpose
    Expresses human ERRbeta2 splice variant in mammalian cells
    Depositor
    Rebecca Riggins
    Article
    Heckler et al Cell Cycle. 2014 Dec 12:0.
    Insert
    Estrogen-related receptor beta (ESRRB Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    wild type sequence; codon optimized to exclude Ec…
    Promoter
    SV40
    Available since
    April 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    52186-plasmid-map-sequence-id-117816

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  36. pUC57 APEX

    Plasmid
    #40306
    Purpose
    A monomeric and improved-activity variant of pea ascorbate peroxidase. Used for electron microscopy and live-cell proteomic mapping. This construct has been replaced by APEX2 (construct 49386).
    Depositor
    Alice Ting
    Article
    Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103
    Insert
    pea APEX (Synthetic)
    Use
    Unspecified
    Tags
    Expression
    Mutation
    K14D, W41F, E112K (relative to wild-type)
    Promoter
    Available since
    Sept. 5, 2012
    Availability
    Academic Institutions and Nonprofits only
    40306-plasmid-map-sequence-id-115990

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  39. pYFAC-CH4

    Plasmid
    #168980
    Purpose
    AMA1 based expression in filamentous fungi.pYFAC-pyroA carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA
    Depositor
    Yit Heng Chooi
    Article
    Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d
    Type
    Empty backbone
    Use
    Synthetic Biology; Heterologous expression in asp…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    168980-plasmid-map-sequence-id-350001

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  42. pYFAC-CH2

    Plasmid
    #168978
    Purpose
    AMA1 based expression in filamentous fungi pYFAC-pyrG carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA
    Depositor
    Yit Heng Chooi
    Article
    Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d
    Type
    Empty backbone
    Use
    Synthetic Biology; Heterologous expression in asp…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    168978-plasmid-map-sequence-id-353766

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  43. pDR-GFPuniv

    Plasmid
    #46085
    Purpose
    In vivo recombination assay fluorescent reporter
    Depositor
    Raymond Monnat
    Article
    Li et al Nucleic Acids Res. 2012 Mar;40(6):2587-9
    Insert
    DR-GFPuniv reporter
    Use
    Recombination reporter plasmid
    Tags
    Expression
    Mammalian
    Mutation
    E5G and K163R mutations in EGFP relative to wild-…
    Promoter
    Available since
    May 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    46085-plasmid-map-sequence-id-64906

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  46. pcDNA3.1-Pert Heavy chain-SpyTag003

    Plasmid
    #216309
    Purpose
    Expresses anti-HER2 Pert Heavy chain-SpyTag003 in mammalian cells. To be paired with Pert light chain to form the anti-HER2 Pert Fab (related to Pertuzumab)
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    H2-18 Heavy chain-SpyTag003
    Use
    Affinity Reagent/ Antibody
    Tags
    His6 tag, Signal peptide, and SpyTag003
    Expression
    Mammalian
    Mutation
    Promoter
    T7 promoter
    Available since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216309-plasmid-map-sequence-id-433372

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  47. LRP6-m5-pCS2-VSVG

    Plasmid
    #27283
    Depositor
    Xi He
    Article
    Tamai et al Mol Cell. 2004 Jan 16. 13(1):149-56.
    Insert
    low density lipoprotein receptor-related protein 6 (LRP6 Human)
    Use
    Xenopus injection
    Tags
    VSVG
    Expression
    Mutation
    Changed S/T of all five PPP(S/T)P motifs to Alani…
    Promoter
    Available since
    March 3, 2011
    Availability
    Academic Institutions and Nonprofits only

  50. pYFAC-CH3

    Plasmid
    #168979
    Purpose
    AMA1 based expression in filamentous fungi pYFAC-riboB carrying PalcA-T1-PalcS/M-T2-PaldA expression cassette ; unique restriction site: PacI for PalcA, NotI for PalcS, AsiSI for PalcM, AscI for PaldA
    Depositor
    Yit Heng Chooi
    Article
    Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d
    Type
    Empty backbone
    Use
    Synthetic Biology; Heterologous expression in asp…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    168979-plasmid-map-sequence-id-350204

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Showing: 1551 - 1600 of 2791 results