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Plasmid#103059Purposemedium copy plasmid (pBR322 backbone) expressing the unstable GFP variant GFP-OVA under control of the nitric oxide-inducible promoter hmpApDepositorInsertGFP-OVA
UseTagsnoneExpressionBacterialMutationPromoterhmpApAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNB23
Plasmid#103058Purposemedium copy plasmid (pBR322 backbone) expressing the unstable GFP variant GFP-OVA under control of the hydrogen peroxide-inducible promoter katGpDepositorInsertGFP-OVA
UseTagsExpressionBacterialMutationPromoterhydrogen peroxide-inducible promoter katGpAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
KBB439
Plasmid#185093PurposeNMA111-GFP wild type under control of GAL1 promoter (complements nma111 deletion)DepositorInsertNMA111
UseTagsGFPExpressionYeastMutationGAL1::NMA111-GFPPromoterGAL1Available sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB462
Plasmid#185095PurposeNMA111-GFP with NLS1 and NLS2 mutated under control of GAL1 promoterDepositorInsertNMA111
UseTagsGFPExpressionYeastMutationGAL1::nma111Dnls1Dnls2-GFPPromoterGAL1Available sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
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eDrTrkA
Plasmid#183996PurposeOptically controlled eDrTrkA expression in mammalian cellsDepositorInserteDrTrkA (NTRK1 Human)
UseTagsIg kappa secretory signal, mCherry, Deinococcus r…ExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
eDrTrkB
Plasmid#183997PurposeOptically controlled eDrTrkB expression in mammalian cellsDepositorInserteDrTrkB (NTRK2 Human)
UseTagsIg kappa secretory signal, mCherry, Deinococcus r…ExpressionMammalianMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NES-SspB-MCS-P2A-iLIDcaax
Plasmid#173869PurposeMammalian expression of mCherry-NES-SspB-P2A-iLIDcaax (opto-control) containing multiple cloning sites (MCS)DepositorInsertmCherry-NES-SspB-MCS-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mCherryExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRC072
Plasmid#225982PurposeJ23110_buj_sfGFP_buj_mRFPDepositorInsertsfGFP (with buj RBS), mRFP (with buj RBS)
UseTagsExpressionBacterialMutationPromoterJ23110Available sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOD1988-epiDEG
Plasmid#89357PurposeMosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pdpy-7 (epidermis specific).DepositorInsertvhhGFP4-ZIF-1 (zif-1 Nematode, Synthetic)
UseTargeting vector for mos1 transposon mediated sin…TagsvhhGFP4 (GFP nanobody)ExpressionMutationPromoterPdpy-7Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOD2046-bwmDEG
Plasmid#89367PurposeMosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pmyo-3 (body wall muscle specific).DepositorInsertvhhGFP4-ZIF-1 (zif-1 Nematode, Synthetic)
UseTargeting vector for mos1 transposon mediated sin…TagsvhhGFP4 (GFP nanobody)ExpressionMutationPromoterPmyo-3Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOD2044-intDEG
Plasmid#89366PurposeMosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pelt-2 (intestine specific).DepositorInsertvhhGFP4-ZIF-1 (zif-1 Nematode, Synthetic)
UseTargeting vector for mos1 transposon mediated sin…TagsvhhGFP4 (GFP nanobody)ExpressionMutationPromoterPelt-2Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOD2048-csnDEG
Plasmid#89368PurposeMosSCI targeting vector expressing a fusion between a GFP nanobody and ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression controlled by Posm-6 (ciliated sensory neuron specific)DepositorInsertvhhGFP4-ZIF-1 (zif-1 Nematode, Synthetic)
UseTargeting vector for mos1 transposon mediated sin…TagsvhhGFP4 (GFP nanobody)ExpressionMutationPromoterPosm-6Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOD2087-trnDEG
Plasmid#89369PurposeMosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pmec-18 (touch neuron specific).DepositorInsertvhhGFP4-ZIF-1 (zif-1 Nematode, Synthetic)
UseTargeting vector for mos1 transposon mediated sin…TagsvhhGFP4 (GFP nanobody)ExpressionMutationPromoterPmec-18Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAT-YFP-TetR
Plasmid#59018PurposeExpresses a fusion of yellow fluorescent protein (YFP) to the N-terminus of the Escherichia coli Tn10 tet repressor (TetR) driven by the alpha tubulin promoter for use in T. gondiiDepositorInsertYFP-TetR
UseToxoplasma expressionTagsExpressionMutationPromoteralpha-tubulinAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFS462
Plasmid#89065Purposehis7 integration plasmid with Z3EVpr:GFPDepositorInsertZ3EV promoter GFP-NLS leu1 C-terminal 300 nt
UseS. pombe his7 integration vectorTagsExpressionMutationPromoterZ3EVAvailable sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_mitoLAMA-F98
Plasmid#130704PurposePlasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusionDepositorInsertNTOM20-SNAP-Tag-LAMA-F98
UseTagsNTOM20 and SNAP-TagExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_mitoLAMA-G97
Plasmid#130705PurposePlasmid encoding outer mitochondrial localization of LAMA-G97 for GFP as a SNAP-Tag-fusionDepositorInsertNTOM20-SNAP-Tag-LAMA-G97
UseTagsNTOM20 and SNAP-TagExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPU-Empty-miR30-L1221
Plasmid#132940PurposeAll-in-one control rescue-control shRNA knockdown vectorDepositorInsertGFP
UseLentiviral and RNAiTagsHAExpressionMammalianMutationPromoterSFFVAvailable sinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJ02B2Gm_AE
Plasmid#66063PurposeMoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Kanamycin plasmid [A:J23102:B:BCD2:C:E0040m:D:B0015:E]DepositorInsertFACS Controls
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJ02B2Gm_EF
Plasmid#66064PurposeMoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Kanamycin plasmid [E:J23102:B:BCD2:C:E0040m:D:B0015:F]DepositorInsertFACS Controls
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_nucLAMA-G97 IRES mYFP
Plasmid#130713PurposePlasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and mYFPDepositorInsertSNAP-Tag-LAMA-G97-NLS IRES mYFP
UseTagsNLS and SNAP-TagExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_nucLAMA-F98 IRES mYFP
Plasmid#130712PurposePlasmid encoding nuclear localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and mYFPDepositorInsertSNAP-Tag-LAMA-F98-NLS IRES mYFP
UseTagsNLS and SNAP-TagExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAHW/Joseph2-Multitagged Plasmid
Plasmid#226725PurposeValidates the expression and detection of epitope-tagged proteins of interest in Drosophila cells.DepositorInsertpAHW/Joseph2
UseDrosophila s2 cell expressionTagsHA, Myc, GFP, Flag, V5, 6xHisExpressionInsectMutationPromoterAct5CAvailable sinceJan. 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJ02B2Gm_AE
Plasmid#66057PurposeMoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Ampicillin plasmid [A:J23102:B:BCD2:C:E0040m:D:B0015:E]DepositorInsertFACS Controls
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJ02B2Gm_EF
Plasmid#66058PurposeMoClo Transcriptional Unit: FACS Standard Color Controls - High GFP expression cassette - Ampicillin plasmid [E:J23102:B:BCD2:C:E0040m:D:B0015:F]DepositorInsertFACS Controls
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
mVenus-NES-SspB-MCS-P2A-iLIDcaax
Plasmid#173864PurposeMammalian expression of mVenus-NES-SspB-P2A-iLIDcaax (opto-control) containing multiple cloning sites (MCS)DepositorInsertmVenus-NES-SspB-MCS-P2A-iLIDcaax
UseTagsC-terminal CAAX-box and mVenusExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
eDrIR1
Plasmid#183998PurposeOptically controlled eDrIR1 expression in mammalian cellsDepositorInserteDrIR1 (INSR Human)
UseTagsIg kappa secretory signal, mCherry, Deinococcus r…ExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRC03
Plasmid#114682PurposeDHFR-sfGFP.Strand11DepositorInsertDHFR-sfGFP.Strand11
UseTagsGSTExpressionMutationPromoterT7 PromoterAvailable sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAM1956
Plasmid#40251DepositorInsertGFPmut2
UseCyanobacteria cloning vectorTagsExpressionMutationPromotern/aAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAM1954
Plasmid#40250DepositorInsertGFPmut2
UseCyanobacteria cloning vectorTagsExpressionMutationPromotern/aAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
Plas-gRNA-Z3EV
Plasmid#157659PurposeGFP gene and gRNA under Z3EV expression control (estradiol sensor), with Z3EV transcription factor to insert in the genome.DepositorInsertGFP-gRNA NatMX Z3EV
UseInsert storage (replicative in e. coli)TagsExpressionMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
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ChR2-2A-Halo
Plasmid#22047PurposeExpression of ChR2-GFP and Halo-YFP for bi-directional control of neuronal activityDepositorInsertChR2-GFP-2A-Halo-YFP
UseTagsGFP and YFPExpressionMammalianMutationNote that there is a S147R mut in YFP. This mutat…PromoterCMVAvailable sinceSept. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
Banshee-ShCit-A
Plasmid#85216PurposeShort hairpin RNAs were designed to target murine Cit (encoding Citron Rho-interacting kinase) and were subcloned into the Banshee-GFP vector for expression under control of the H1 promoter.DepositorInsertshCit-A
UseRetroviralTagsExpressionMammalianMutationPromoterH1Available sinceFeb. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
Banshee-ShCit-B
Plasmid#85217PurposeShort hairpin RNAs were designed to target murine Cit (encoding Citron Rho-interacting kinase) and were subcloned into the Banshee-GFP vector for expression under control of the H1 promoter.DepositorInsertshCit-B
UseRetroviralTagsExpressionMammalianMutationPromoterH1Available sinceFeb. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDUP1
Plasmid#223516Purposeinactive cogfp under control of Ptet, cogfp under control of PtacDepositorArticleInsertsCoGFP
CoGFP inactivated
UseTagsHis tagExpressionBacterialMutationanti-dimerization mutations S129G, 22 C154S, D156…PromoterPtac, Ptac and Ptet, and PtetAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDUP2
Plasmid#223517Purposecogfp copy 1 under control of Ptet, cogfp copy 2 under control of PtacDepositorArticleInsertsCoGFP
CoGFP (second copy)
UseTagsHis tagExpressionBacterialMutationanti-dimerization mutations S129G, 22 C154S, D156…PromoterPtac, Ptac and Ptet, and PtetAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAP-U2-3
Plasmid#236474PurposePlasmid for integration at the native URA3 locus with 3xmCherry under control of the ScACT1 promoter and 3xGFP under control of the ApACT1 promoter.DepositorInsertscACT1p-3xmCherry apACT1p-3xGFP
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceApril 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAP-U2-4
Plasmid#236475PurposePlasmid for integration at the native URA3 locus with 3xmCherry under control of the ScACT1 promoter and 3xGFP under control of the ApTUB1 promoter.DepositorInsertscACT1p-3xmCherry apTUB1p-3xGFP
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAP-U2-2
Plasmid#236473PurposePlasmid for integration at the native URA3 locus with 3xmCherry under control of the SpH2B promoter and 3xGFP under control of the ScACT1 promoter.DepositorInsertspH2Bp-3xmCherry scACT1p-3xGFP
UseTagsExpressionYeastMutationCodon optimized for A. pullulansPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
Z-lock cofilin
Plasmid#141137Purposecontrolling the actin-severing ability of cofilin with lightDepositorInsertZdk2, Cofilin, LOV2
UseTagsExpressionMammalianMutationmCherry, Zdk2(132F), Cofilin S3A, LOV WTPromoterAvailable sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
Mammalian expression: Z-lock cofilin dark state mutant
Plasmid#141136Purposea control for optogenetic cofilin analogDepositorInsertZdk2, Cofilin, LOV2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
eDrEGFR
Plasmid#184000PurposeOptically controlled eDrEGFR expression in mammalian cellsDepositorInserteDrEGFR (EGFR Human)
UseTagsIg kappa secretory signal, mCherry, Deinococcus r…ExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
eDrcKIT
Plasmid#184001PurposeOptically controlled eDrcKIT expression in mammalian cellsDepositorInserteDrcKIT (KIT Human)
UseTagsIg kappa secretory signal, mCherry, Deinococcus r…ExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
AktPH-mCherry-T2A-PHR-T2A-CIBNcaax
Plasmid#173861PurposeMammalian expression of Akt PH-mCherry-T2A-PHR-T2A-CIBNcaax (PIP3 marker and opto-control)DepositorInsertAktPH-mCherry-T2A-PHR-T2A-CIBNcaax
UseTagsC-terminal CAAX-box and mCherryExpressionMammalianMutationPromoterCAGAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
eDrMet
Plasmid#183999PurposeOptically controlled eDrcMet expression in mammalian cellsDepositorInserteDrMet (MET Human)
UseTagsIg kappa secretory signal, mCherry, Deinococcus r…ExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRC01
Plasmid#114680PurposesfGFP.Strands 1-9DepositorInsertsfGFP Strands 1-9
UseTagsGSTExpressionBacterialMutationPromoterT7Available sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRC02
Plasmid#114681PurposesfGFP.Strand10-HaloenzymeDepositorInsertsfGFP.Strand10-Haloenzyme
UseTagsHis10ExpressionBacterialMutationPromoterT7Available sinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
908H
Plasmid#160240PurposeTetO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attBDepositorInsertsTet Operator (2x)
hsp70 minimal promoter
p-element promoter
Firefly luciferase
Renilla luciferase
Ubiquitin promoter
UseTagsT2A GFPExpressionInsectMutationPromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only