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gag-PE

Plasmid

#211371

Purpose

Packaging plasmid for v3 PE-eVLP production

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

MMLVgag-PE

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET21a-MTF-His

Plasmid

#124133

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Methionyl-tRNA formyltransferase (fmt Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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p4027 GST-ubiquitin

Plasmid

#10861

Depositor

Peter Howley

Article

Scheffner et al Cell. 1993 Nov 5. 75(3):495-505.

Insert

ubiquitin (UBI4 Budding Yeast)

Tags

GST

Expression

Bacterial

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
p4RTK GL4.10 (Luc2)

Plasmid

#16057

Depositor

Robert Benezra

Article

Tournay et al Mol Cell Biol. 1996 May . 16(5):2418-30.

Insert

Multimerized E-box

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3 MLL2 653

Plasmid

#11017

Depositor

Matthew Meyerson

Article

Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

Insert

MLL2 (KMT2B Human)

Tags

FLAG

Expression

Mammalian

Mutation

truncation of MLL2, last 653 amino acids, from pr…

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pet-15b-ultraID

Plasmid

#172879

Purpose

expresses ultraID in E. coli

Depositor

Julien Béthune

Article

Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

Insert

ultraID

Tags

myc

Expression

Bacterial

Mutation

fragment ([aa2-171]) of aquifex aeolicus BirA wit…

Promoter

T7

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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p3x-FLAG-hnRNPF

Plasmid

#21926

Depositor

Mariano Garcia-Blanco

Article

Mauger et al Mol Cell Biol. 2008 Sep . 28(17):5403-19

Insert

hnRNPF (HNRNPF Human)

Tags

3 Flags

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2009

Availability

Academic Institutions and Nonprofits only

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pJMP1

Plasmid

#79873

Purpose

Bacillus subtilis dCas9 expression vector; integrates into lacA/ganA

Depositor

Carol Gross, Jason Peters, Stanley Qi

Article

Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

Insert

dCas9

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

xylA

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/FLAG/HA-DEST TNRC6A

Plasmid

#19883

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

Trinucleotide repeat containing 6A (TNRC6A Human)

Tags

FLAG/HA

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pBT374

Plasmid

#125615

Purpose

Expresses evoFERNY-BE4max-NG in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

evoFERNY-BE4max-NG

Expression

Mammalian

Mutation

H102P D104N

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO V5

Plasmid

#19445

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

V5

Expression

Mammalian

Mutation

V5 tag inserted to create new vector

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 NSP10

Plasmid

#141264

Purpose

Gateway-compatible Entry vector, with insert of NSP10 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP10 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

April 3, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV-2 ORF6

Plasmid

#141275

Purpose

Gateway-compatible Entry vector, with insert of ORF6 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF6 (ORF6 SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR4_G3BP2

Plasmid

#127105

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

G3BP2 (G3BP2 Human)

Use

Gateway Cloning

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR207 SARS-CoV-2 ORF7A

Plasmid

#141276

Purpose

Gateway-compatible Entry vector, with insert of ORF7A gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

ORF7A (ORF7a SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pEVS104

Plasmid

#207393

Purpose

Conjugal helper plasmid

Depositor

Jason Peters, Edward Ruby, Eric Stabb

Article

Hall et al MicroPubl Biol. 2023 Jul 13;2023:10.1791

Type

Empty backbone

Expression

Bacterial

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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HA-Vps34

Plasmid

#86749

Purpose

N-terminal HA-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

Insert

human Vps34 (PIK3C3 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pNICE-NL2(-)

Plasmid

#15246

Depositor

Peter Scheiffele

Article

Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

Insert

Neuroligin 2(-) (Nlgn2 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Jan. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV enAsCas12f-HKRA-sgRNA_v7

Plasmid

#208357

Purpose

Expression of enAsCas12f-HKRA and sgRNA_v7 in mammalian cells

Depositor

Atsushi Hoshino

Article

Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

Insert

enAsCas12f-HKRA

Expression

Mammalian

Mutation

I123H/D195K/D208R/V232A

Promoter

CMV

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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ACTN2-pEGFP

Plasmid

#52669

Purpose

Overexpression of GFP-tagged α-actinin-2

Depositor

Johannes Hell

Article

Hall et al Neuron. 2013 May 8;78(3):483-97. doi: 10

Insert

actinin 2 (ACTN2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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