We narrowed to 7,351 results for: PAC

1. pACYC-Cas12g1-noncoding

   Plasmid

   #120880

   Purpose

   Expresses concatenated noncoding sequences surrounding the CRISPR-Cas12g1 system in the native genomic loci; expresses the tracrRNA required for activity.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

   Insert

   Noncoding sequences for CRISPR-Cas12g1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pF143 pAcGFP1 SOD1G37R

   Plasmid

   #26404

   Depositor

   Elizabeth Fisher

   Article

   Stevens et al PLoS One. 2010 . 5(3):e9541.

   Insert

   SOD1 (SOD1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   G37R

   Promoter

   Available since

   Nov. 8, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pX459-HypaCas9-TUBA1B_sgRNA

   Plasmid

   #183889

   Purpose

   pX459V2.0-HypaCas9 plasmid with TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   TUBA1B sgRNA spacer (TUBA1B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAC147-pCR8-dCas9VP64

   Plasmid

   #48219

   Purpose

   dCas9VP64 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression 

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9(D10A;H840A) fusion with VP64 activation domain
   

   Use

   CRISPR; Gateway donor

   Tags

   HA Tag and VP64

   Expression

   Mutation

   D10A;H840A

   Promoter

   Available since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. INCTbiosyn-pAct-AtINTBxb1

   Plasmid

   #127536

   Purpose

   Plasmid encodes A. thaliana codon optimized Integrase Bxb1.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   Integrase Bxb1 coding sequence codon optimized for A. thaliana expression.
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. IL2 F42A pAcGP67a

   Plasmid

   #41807

   Depositor

   Chris Garcia

   Article

   Levin et al Nature. 2012 Mar 25;484(7395):529-33. do

   Insert

   IL2 (IL2 Human)
   

   Use

   Tags

   6x His

   Expression

   Insect

   Mutation

   Phenylalanine 42 to Alanine

   Promoter

   Polyhedron

   Available since

   Jan. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pAC-EsaR-V220A

   Plasmid

   #47645

   Purpose

   pAC-EsaR with a c659t mutation in esaR

   Depositor

   Cynthia Collins

   Article

   Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

   Insert

   esaR-V220A
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Changed Valine 220 to Alanine

   Promoter

   Plac

   Available since

   Sept. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pAC8_GWs-Lox (VE5589)

   Plasmid

   #163769

   Purpose

   Transfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette under the pH promoter.

   Depositor

   Arnaud Poterszman

   Article

   Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

   Insert

   no insert
   

   Use

   Tags

   no tag

   Expression

   Insect

   Mutation

   Promoter

   PH

   Available since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAC4 Frk-SH2

   Plasmid

   #46428

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Frk (FRK Human)
   

   Use

   Tags

   AviTag, GST, and Thrombin site

   Expression

   Bacterial

   Mutation

   contains amino acids 108-213

   Promoter

   Trc

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAc5.1B-EGFP-DmDCP1

    Plasmid

    #21684

    Depositor

    Elisa Izaurralde

    Article

    Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

    Insert

    Decapping protein 1 (Dcp1 Fly)
    

    Use

    Drosophila expression

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pACEBac1-beta-actin

    Plasmid

    #178076

    Purpose

    Insect cell expression of beta-actin

    Depositor

    Tarun Kapoor

    Article

    Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

    Insert

    beta-actin (ACTB Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pACYC-Cas12c2-noncoding

    Plasmid

    #120877

    Purpose

    Expresses concatenated noncoding sequences surrounding the CRISPR-Cas12c2 system in the native genomic loci; expresses the tracrRNA required for activity.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

    Insert

    Noncoding sequences for CRISPR-Cas12c2
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPD288 ZF43x4-Compact_EYFP

    Plasmid

    #138899

    Purpose

    EYFP reporter vector for the ZF43x4-Compact promoter

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x4-Compact
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ZF43x4-Compact

    Available since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEJS1026-pMCSG7-HpaCas9

    Plasmid

    #121540

    Purpose

    Expresses a 6X-His tagged type II-C Cas9 from H. parainfluenzae in bacterial cells

    Depositor

    Erik Sontheimer

    Article

    Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

    Insert

    HpaCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAC92-pmax-dCas9VP96

    Plasmid

    #48224

    Purpose

    dCas9VP96 on pmax expression vector

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP96 activation domain
    

    Use

    CRISPR

    Tags

    HA Tag and VP96

    Expression

    Mammalian

    Mutation

    D10A;H840A

    Promoter

    CAGGS

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAC1809_pmax-RBM38-FRB

    Plasmid

    #118642

    Purpose

    Transient transfection; Expresses RBM38-FRB; CAGGS promoter

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    RBM38-FRB
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAc5.1B-EGFP-DmGW182

    Plasmid

    #22419

    Depositor

    Elisa Izaurralde

    Article

    Behm-Ansmant et al Genes Dev. 2006 Jul 15. 20(14):1885-98.

    Insert

    gawky, variant A (gw Fly)
    

    Use

    Drosophila expression

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. Cip-pAct1-Mnase

    Plasmid

    #162556

    Purpose

    NheI-MluI-digested 3xFLAG-MNase-SV40 fragment was cloned into the CIp-pACT1-CYC vector to ensure constitutive expression of MNase in C. albicans.

    Depositor

    Adnane Sellam

    Article

    Tebbji et al mSphere. 2020 Oct 14;5(5). pii: 5/5/e006

    Insert

    3FLAG-Mnase-SV40
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Actine

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAc5.1B-lambdaN-HA-DmStaufen_B

    Plasmid

    #145947

    Purpose

    Insect Expression of DmStaufen

    Depositor

    Elisa Izaurralde

    Article

    Behm-Ansmant et al Genes Dev. 2006 Jul 15. 20(14):1885-98.

    Insert

    DmStaufen (stau Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAcGFP1-N1-Dsup-deltaC

    Plasmid

    #90023

    Purpose

    To express GFP-tagged Dsup lacking C-terminal region in mammalian cells transiently

    Depositor

    Takekazu Kunieda

    Article

    Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

    Insert

    Dsup
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Deletion of C-terminal region

    Promoter

    CMV

    Available since

    Aug. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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