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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,026 results for: Psp

Showing: 621 - 640 of 1026 results

  1. pDB007ns2a

    Plasmid
    #99214
    Purpose
    Accessory plasmid for PACE of PylRS variants; negative selection
    Depositor
    David Liu
    Article
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); Ptet [SD4] T7RNAP(S12*,S203*)
    Use
    Tags
    Expression
    Mutation
    amber codons at positions 12 and 203 of T7 RNA po…
    Promoter
    Ppsp, PproK, and Ptet
    Available Since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    99214-plasmid-map-sequence-id-192369

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  2. pDB023f1

    Plasmid
    #99218
    Purpose
    Complementary plasmid for aaRS PACE; amber codons in T7 RNAP
    Depositor
    David Liu
    Article
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD4] T7RNAP(S12*,S203*)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    amber codons at positions 12 and 203 of T7 RNA po…
    Promoter
    Ppsp
    Available Since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    99218-plasmid-map-sequence-id-192402

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  3. pDB026e

    Plasmid
    #109261
    Purpose
    Accessory plasmid for PACE of MjTyrRS variants
    Depositor
    David Liu
    Article
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp_pIII(P29*, Y184*); luxAB; PproK_TyrT(Opt, CUA);
    Use
    Tags
    Expression
    Bacterial
    Mutation
    amber codon at position 29 and 184 of gIII
    Promoter
    Ppsp and PproK
    Available Since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    109261-plasmid-map-sequence-id-210479

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  4. pDB023k

    Plasmid
    #99219
    Purpose
    Complementary plasmid for aaRS PACE; amber codons in T7 RNAP
    Depositor
    David Liu
    Article
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD8] T7RNAP(S12*,S203*,S527*)
    Use
    Tags
    Expression
    Mutation
    amber codons at positions 12, 203, and 527 of T7 …
    Promoter
    Ppsp
    Available Since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    99219-plasmid-map-sequence-id-192936

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  5. pDB026g

    Plasmid
    #109263
    Purpose
    Accessory plasmid for PACE of MjTyrRS variants
    Depositor
    David Liu
    Article
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp_pIII(P29*, P83*, T177*, Y184*); luxAB; PproK_TyrT(Opt, CUA);
    Use
    Tags
    Expression
    Bacterial
    Mutation
    amber codon at position 29, 83, 177, and 184 of g…
    Promoter
    Ppsp and PproK
    Available Since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    109263-plasmid-map-sequence-id-210480

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  6. AAA

    Plasmid
    #78487
    Purpose
    2135nt RNA encoded by chimaeric gene cloned in a pSP65A vector that included about 65 dT residues for transcription of a poly(A) tail
    Depositor
    Allan Jacobson
    Article
    Amrani et al Nature. 2008 Jun 26;453(7199):1276-80. d
    Insert
    CAN1-LUC
    Use
    Tags
    Expression
    Bacterial
    Mutation
    CAN1 contains no stop codon
    Promoter
    Available Since
    Aug. 31, 2016
    Availability
    Academic Institutions and Nonprofits only

  10. pC0051-W85X REPAIR targeting guide cloned into pC0043

    Plasmid
    #103867
    Purpose
    Guide RNA expression plasmid for dPspCas13b that can be used to direct ADAR deaminase domain activity to the W85X mutation in the luciferase reporter plasmid pC0038
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Insert
    W85X targeting guide RNA
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    103867-plasmid-map-sequence-id-199148

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  14. pDB036d

    Plasmid
    #99216
    Purpose
    Accessory plasmid for PACE of MjTyrRS variants; negative selection
    Depositor
    David Liu
    Article
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); PproA [SD4] T7RNAP(S12*,S203*)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    amber codons at positions 12 and 203 of T7 RNA po…
    Promoter
    Ppsp, PproK, and PproA
    Available Since
    Nov. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    99216-plasmid-map-sequence-id-198818

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  15. pDB036a

    Plasmid
    #99215
    Purpose
    Accessory plasmid for PACE of MjTyrRS variants; negative selection
    Depositor
    David Liu
    Article
    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038
    Insert
    Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); PproD [SD4] T7RNAP(S12*,S203*)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    amber codons at positions 12 and 203 of T7 RNA po…
    Promoter
    Ppsp, PproK and PproD
    Available Since
    Oct. 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    99215-plasmid-map-sequence-id-192376

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  17. pSLQ10530

    Plasmid
    #239266
    Purpose
    Expresses NES-dCas13b-ABI for CRISPR-TO perturbation in the cytoplasm of cell lines
    Depositor
    Stanley Qi
    Article
    Han et al Nature. 2025 Jul;643(8070):241-251. doi:
    Insert
    NES-dPspCas13b-EGFP-ABI
    Use
    CRISPR and Lentiviral
    Tags
    Flag tag and V5 tag
    Expression
    Mammalian
    Mutation
    H133A and H1058A in PspCas13b
    Promoter
    EF1a
    Available Since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    239266-plasmid-map-sequence-id-474747

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  18. MSCV-BCR-ABL1-p190-IRES-EGFP

    Plasmid
    #219964
    Purpose
    Express human BCR-ABL1-P190 in mammalian cells
    Depositor
    Mohamed Fareh
    Insert
    BCR-ABL1-P190 (ABL1 Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Sept. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    219964-plasmid-map-sequence-id-442484

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  19. pSLQ10625

    Plasmid
    #239268
    Purpose
    Expresses NES-dCas13b-ABI for CRISPR-TO perturbation in primary mouse cortical neurons
    Depositor
    Stanley Qi
    Article
    Han et al Nature. 2025 Jul;643(8070):241-251. doi:
    Insert
    NES-dPspCas13b-EGFP-ABI
    Use
    Tags
    Flag tag and V5 tag
    Expression
    Mammalian
    Mutation
    H133A and H1058A in PspCas13b
    Promoter
    CAG
    Available Since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    239268-plasmid-map-sequence-id-474742

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  20. pSLQ10531

    Plasmid
    #239267
    Purpose
    Expresses NLS-dCas13b-ABI for CRISPR-TO perturbation in the nucleus of cell lines
    Depositor
    Stanley Qi
    Article
    Han et al Nature. 2025 Jul;643(8070):241-251. doi:
    Insert
    NLS-dPspCas13b-EGFP-ABI
    Use
    CRISPR and Lentiviral
    Tags
    Flag tag and V5 tag
    Expression
    Mammalian
    Mutation
    H133A and H1058A in PspCas13b
    Promoter
    EF1a
    Available Since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    239267-plasmid-map-sequence-id-475551

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Showing: 621 - 640 of 1026 results