We narrowed to 59,259 results for: cloning

1. pD(Nt)_His6-GST-TEV

   Plasmid

   #223878

   Purpose

   4G-cloning donor for N-terminal His6-GST-TEV tag

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   His6-GST-TEV
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pD(Nt)_His6-MBP-TEV

   Plasmid

   #223877

   Purpose

   4G-cloning donor for N-terminal His6-MBP-TEV tag

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   His6-MBP-TEV
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pD(Nt)_His6-HA2-TEV

   Plasmid

   #223876

   Purpose

   4G-cloning donor for N-terminal His6-HA2-TEV tag

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   His6-HA2-TEV
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pD(Nt)_His6-FLAG3-TEV

   Plasmid

   #223875

   Purpose

   4G-cloning donor for N-terminal His6-FLAG3-TEV tag

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   His6-FLAG3-TEV
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pD(P+T)_phC

   Plasmid

   #223859

   Purpose

   4G-cloning donor for insect promoter and terminator, C-terminal tag added

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   polyhedrin promoter and SV40 polyA
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pD(P+T)_T7N

   Plasmid

   #223855

   Purpose

   4G-cloning donor for bacterial promoter and terminator, N-terminal tag added

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   T7 promoter and terminator
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pD(P+T)_T7C

   Plasmid

   #223856

   Purpose

   4G-cloning donor for bacterial promoter and terminator, C-terminal tag added

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   T7 promoter and terminator
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pD(Nt)_His10-TS-3C

   Plasmid

   #223884

   Purpose

   4G-cloning donor for N-terminal His10-TwinStrep-3C tag

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   His10-TwinStrep-3C
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pD(Ct)_3C-Venus-His8

   Plasmid

   #223889

   Purpose

   4G-cloning donor for C-terminal 3C-Venus-His8 tag

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   3C-Venus-His8
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pD(P+T)_CMVC

    Plasmid

    #223862

    Purpose

    4G-cloning donor for mammalian promoter and terminator, C-terminal tag added

    Depositor

    Stephan Gruber

    Article

    Taschner et al bioRxiv 2024.06.17.599261

    Insert

    CMV promoter and bGH polyA
    

    Use

    Golden-gate donor

    Available Since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. p667-UBC-STK17A-V5-miniTurbo_IDG-K

    Plasmid

    #214466

    Purpose

    Gateway destination clone of STK17A (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    STK17A-V5-miniTurbo (STK17A Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. YIp LYS2-MET17

    Plasmid

    #122910

    Purpose

    LYS2-MET17 integrating (YIp) vector for stable genomic integration in the BY4741 background.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Expression

    Yeast

    Availability

    Academic Institutions and Nonprofits only

    

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13. YIp LYS2-URA3

    Plasmid

    #122911

    Purpose

    LYS2-URA3 integrating (YIp) vector for stable genomic integration in the BY4741 background.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Expression

    Yeast

    Availability

    Academic Institutions and Nonprofits only

    

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14. YIp TRP1-LEU2

    Plasmid

    #122913

    Purpose

    TRP1-LEU2 integrating (YIp) vector for stable genomic integration in the BY4741 background.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Expression

    Yeast

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. YIp TRP1-MET17

    Plasmid

    #122914

    Purpose

    TRP1-MET17 integrating (YIp) vector for stable genomic integration in the BY4741 background.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Expression

    Yeast

    Availability

    Academic Institutions and Nonprofits only

    

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16. YIp LYS2-KanMx

    Plasmid

    #122920

    Purpose

    LYS2-KanMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Expression

    Yeast

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLEX_307

    Plasmid

    #41392

    Purpose

    Constitutive lentiviral expression, SV40-puro; EF1a-gateway-V5 tag

    Depositor

    David Root

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Nov. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. R777-E185 Hs.PLXNB1

    Plasmid

    #70469

    Purpose

    Gateway ORF clone of human PLXNB1 [NM_001130082.2] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    PLXNB1 (PLXNB1 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. R777-E221 Hs.RALGDS

    Plasmid

    #70505

    Purpose

    Gateway ORF clone of human RALGDS [NM_006266.3] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    RALGDS (RALGDS Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDH-UBC-irfp luc-EF1 Hygro

    Plasmid

    #129438

    Purpose

    express irfp-luc chimera

    Depositor

    Oskar Laur

    Insert

    irfp-luc
    

    Use

    Lentiviral

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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