We narrowed to 162,999 results for: addgene

1. pBA407

   Plasmid

   #85970

   Purpose

   UPRE-mCh-Ubc-Neo

   Depositor

   Jonathan Weissman

   Article

   Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

   Inserts

   mCherry
   Neo
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-sgRNA(lib)-puro

   Plasmid

   #119976

   Purpose

   Lenti sgRNA cloning backbone with CMV-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Wensheng Wei

   Article

   Liu et al Nat Biotechnol. 2018 Nov 5. pii: nbt.428

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-EF1a-double floxed-Gat1-HA-WPRE-HGHpA

   Plasmid

   #184635

   Purpose

   Expresses the GABA membrane transporter Gat1 fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

   Depositor

   Nicolas Tritsch

   Article

   Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

   Insert

   Gat1 (Slc6a1 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. IFNAR1_pCSdest

   Plasmid

   #53881

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   IFNAR1 (IFNAR1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SP6 and CMV

   Available since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pFETCh_AHR

   Plasmid

   #101071

   Purpose

   Donor vector for 3' FLAG tag of human AHR

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   AHR homology arms (AHR Human)
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6-sgRNA Ef1alpha Puro-T2A-GFP

   Plasmid

   #111596

   Purpose

   sgRNA Ef1alpha Puro-T2A-GFP

   Depositor

   Jonathan Weissman

   Article

   Horlbeck et al Cell. 2018 Aug 9;174(4):953-967.e22. doi

   Insert

   mU6-sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-SFFV-RTN3-WPRE-UbC-Emerald

   Plasmid

   #233467

   Purpose

   Lentiviral vector plasmid expressing human reticulon 3 (RTN3) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Bart Nieuwenhuis, Richard Eva, Evan Reid

   Inserts

   RTN3 (RTN3 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV and UbC

   Available since

   March 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDH-EF1-FHC-POLQ

   Plasmid

   #64875

   Purpose

   lentiviral expression of human POLQ

   Depositor

   Richard Wood

   Article

   Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

   Insert

   POLQ (POLQ Human)
   

   Use

   Lentiviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Aug. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBA409

   Plasmid

   #85971

   Purpose

   EF1a-sfGFP-Ubc-Neo

   Depositor

   Jonathan Weissman

   Article

   Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

   Inserts

   sfGFP
   Neo
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLIK Tgfbr3-HA neo

    Plasmid

    #58508

    Purpose

    Lentiviral expression vector for an inducible HA-tagged mouse Tgfbr3

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    Tgfbr3_HA (Tgfbr3 Mouse)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

11. mCherry-DRP1 K38A

    Plasmid

    #232350

    Purpose

    Expresses K38A variant of human DRP1 with mCherry tag

    Depositor

    Samantha Lewis

    Article

    Begeman et al bioRxiv [Preprint]. 2024 Aug 17:2024.08.

    Insert

    DRP1 (DNM1L Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    K38A

    Promoter

    CMV

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. qTAG-AAVS1-Ef1a-Puro-moxGFP

    Plasmid

    #227273

    Purpose

    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-moxGFP cassette (AAVS1 Synthetic)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAVS1-Puro CAG PSMA nanobody T-CAR

    Plasmid

    #171962

    Purpose

    Donor plasmid for PSMA nanobody T-CAR expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Harris et al J Immunol Regen Med. 2023 May;20:100074.

    Insert

    Synthetic anti-PSMA nanobody CAR
    

    Use

    CRISPR, Synthetic Biology, and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. plRL2

    Plasmid

    #163631

    Purpose

    Optimized M. tuberculosis CRISPRi plasmid; L5 integrating.

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tet-On

    Available since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNS35 (multiAsCas12a piggyBac)

    Plasmid

    #217336

    Purpose

    piggyBac expression of multiAsCas12a

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    multiAsCas12a
    

    Use

    CRISPR

    Tags

    6xMycNLS and HA-SV40NLS-P2A-TagBFP2

    Expression

    Mammalian

    Mutation

    R1226A/E174R/S542R/K548R

    Promoter

    CAG

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLX302 MX1-V5 puro

    Plasmid

    #158640

    Purpose

    Lentiviral expression vector for constitutive expression of V5-tagged MX1.

    Depositor

    Kevin Janes

    Article

    Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

    Insert

    Mx1 (MX1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-mPGK-eGFP (beta-globin intron)

    Plasmid

    #162513

    Purpose

    AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the mouse phosphoglycerate kinase (mPGK) promoter that is upstream of a beta-globin intron

    Depositor

    Bart Nieuwenhuis

    Article

    Nieuwenhuis et al Gene Ther. 2020 Jun 23. pii: 10.1038/s41

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mPGK

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAC154-dual-dCas9VP160-sgExpression

    Plasmid

    #48240

    Purpose

    Dual expression construct expressing both dCas9VP160 and sgRNA from separate promoters

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    HA-Tag, HA-tag, and VP160

    Expression

    Mammalian

    Mutation

    D10A H840A

    Promoter

    Available since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT3TS-Cavin4b-TurboID

    Plasmid

    #163851

    Purpose

    In vitro transcription of Cavin4b-TurboID. Parton lab clone JTA

    Depositor

    Rob Parton

    Article

    Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

    Insert

    Cavin4b (cavin4b Zebrafish)
    

    Use

    In vitro transcription of rna

    Tags

    TurboID

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. QPM-sgR (pTaU3)

    Plasmid

    #140448

    Purpose

    For sgRNA expression in monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Lin et al Nat Biotechnol. 2020 May;38(5):582-585.

    Insert

    TaU3-sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    TaU3

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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