59,541 results

1. pINDUCER-21-RUNX1

   Plasmid

   #97043

   Purpose

   Expresses RUNX1 in mammalian cells

   Depositor

   George Daley

   Article

   Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

   Insert

   Runt Related Transcription Factor 1 (RUNX1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Tet on

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

2. pAAV-GFAP-hChR2(H134R)-mCherry

   Plasmid

   #27055

   Purpose

   AAV expression of humanized ChR2(H134R) fused to mCherry, for optogenetic excitation

   Depositor

   Karl Deisseroth

   Insert

   hChR2(H134R)
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   GFAP

   Available Since

   Jan. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. RNAPII_CTD27-52

   Plasmid

   #98678

   Purpose

   Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

   Depositor

   Nicolas Fawzi

   Article

   Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

   Insert

   RNA polymerase II C-terminal domain (POLR2A Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCherry-Calnexin-N-14

   Plasmid

   #55005

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Calnexin (CANX Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   I474N in Calnexin

   Promoter

   CMV

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. Stx5S-mCherry

   Plasmid

   #165087

   Purpose

   Expresses Stx5S-mCherry in mammalian cells

   Depositor

   Geert van den Bogaart

   Article

   Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

   Insert

   Stx5 (Short isoform) (STX5 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Lacks amino acids 1-54, enocdes short isoform of …

   Promoter

   CMV

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pC-KIT8

   Plasmid

   #118990

   Purpose

   Mutational dissection of the G rich region of the c-kit promoter

   Depositor

   Bjorn Hogberg

   Article

   Ducani et al J Am Chem Soc. 2019 Jul 3;141(26):10205-

   Insert

   c-kit proto-oncogene {promoter} (KIT Human)
   

   Expression

   Mammalian

   Mutation

   mutK2-mutSP-mutK1

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221_SLC16A9

   Plasmid

   #131876

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC16A9 (SLC16A9 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 9, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. AAV-U6-dgRNA-CAG-MPH

   Plasmid

   #106259

   Purpose

   Expresses MPH co-activator from the CAG promoter and one U6-driven dgRNA in AAV backbone

   Depositor

   Juan Belmonte

   Article

   Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

   Insert

   MS2-P65-HSF1 (HSF1 Synthetic, Human)
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-miniDq-P2A-mCherry

   Plasmid

   #204357

   Purpose

   AAV vector for miniDq expression under the control of human synapsin promoter

   Depositor

   Masao Doi

   Article

   Miyake et al Cell Rep Methods. 2024 Oct 21;4(10):1008

   Insert

   miniDq-P2A-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Mutation

   The third intracellular loop (ICL3) of hM3Dq was …

   Promoter

   hSyn

   Available Since

   Oct. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEF1a_CD WT TET1

    Plasmid

    #124082

    Purpose

    Expression of wild-type catalytic domain only form of TET1

    Depositor

    Silvia Monticelli

    Article

    Vincenzetti et al Eur J Immunol. 2019 Jan 30. doi: 10.1002

    Insert

    TET1 catalytic domain (TET1 Human)
    

    Tags

    Flag and HA

    Expression

    Mammalian

    Promoter

    EF1 alpha

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-TO-NGN2-EMX1 BSD-mApple

    Plasmid

    #182311

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 and EMX1 expression, bsd selection, mApple

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Inserts

    hNGN2-P2A-EMX1 (NEUROG2, EMX1 Human)
    bsd-T2A-mycNLS-mApple
    

    Tags

    T2A-mycNLS-mApple

    Expression

    Mammalian

    Promoter

    EF1a and TRE3G

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFETCh_KLF9

    Plasmid

    #86286

    Purpose

    Donor vector for 3' FLAG tag of human KLF9

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    KLF9 homology arms (KLF9 Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. PX458_KLF9_2

    Plasmid

    #86347

    Purpose

    Encodes gRNA for 3' target of human KLF9

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against KLF9 (KLF9 Human)
    

    Use

    CRISPR

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. PX458_KLF9_1

    Plasmid

    #86346

    Purpose

    Encodes gRNA for 3' target of human KLF9

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against KLF9 (KLF9 Human)
    

    Use

    CRISPR

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pnEA-vH_His-TEV-SEPT2-msfGFP

    Plasmid

    #174492

    Purpose

    bacterial expression of human SEPT2 fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Insert

    SEPTIN2 (SEPTIN2 Human)
    

    Tags

    His6-TEV and msfGFP

    Expression

    Bacterial

    Available Since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pACS129

    Plasmid

    #204594

    Purpose

    Histone H3.1-V5

    Depositor

    Tom Misteli

    Article

    Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

    Insert

    histone H3.1 (H3C3 Human)
    

    Use

    Lentiviral

    Tags

    human histone H3.1-V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL3-sgSEC61B.N1-CAG-Cas9-T2A-mCherry-P2A-Puro

    Plasmid

    #216251

    Purpose

    Express Cas9 with CAG promoter to improve the expression in hES cells and sgSEC61B.N1 to tag endogenous SEC61B N-terminus

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    Cas9 and sgSEC61B.N1 (SEC61B Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. AIMTOR A757

    Plasmid

    #140829

    Purpose

    AIMTOR BRET Biosensor containing a mutated non-phosphorylable ULK1 peptide to use as a control constuct in parrallel with AIMTOR T757

    Depositor

    Anne Bonnieu

    Article

    Bouquier et al BMC Biol. 2020 Jul 3;18(1):81. doi: 10.1

    Insert

    AIMTOR A757 (ULK1 Human)
    

    Tags

    NES Nuclear export signal

    Expression

    Mammalian

    Mutation

    The insert comprises only a small sequence of hum…

    Available Since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAMS823 His6-MBP-3C-ORF2p (238-1061, ORFeus-Hs) in pET41

    Plasmid

    #213024

    Purpose

    Expresses human LINE-1 ORF2p Core (238-1061) in E. coli as an N-MBP fusion

    Depositor

    Martin S. Taylor, Oliver Weichenrieder

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    LINE-1 ORF2p (238-1061)
    

    Tags

    His6-MBP-3C

    Expression

    Bacterial

    Promoter

    T7 / Lac

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a(+)_6xHIS-hHLA-A2 (MHC Class I heavy)-C-Tag

    Plasmid

    #187356

    Purpose

    Bacterial expression and refolding of gp100 pMHC

    Depositor

    Simon Davis

    Article

    Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

    Insert

    hHLA-A2 (MHC Class I heavy) with N-terminal 6xHIS tag, and C-terminal C-Tag
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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