We narrowed to 10,197 results for: otos

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ApoE3_mCh-SspB (pBS1144)

Plasmid

#185326

Purpose

For the mammalian expression of the human protein ApoE3 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE3

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-CCre-MBP4-WPRE

Plasmid

#193919

Purpose

Expresses one of the components of Cre-DOR_N5C4 (RFP-dependent Cre)

Depositor

Tatsushi Onaka

Article

Inutsuka et al Commun Biol. 2022 Sep 16;5(1):979. doi:

Insert

CCre-MBP4

Use

AAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-NCre-MBP5-WPRE

Plasmid

#193918

Purpose

Expresses one of the components of Cre-DOR_N5C4 (RFP-dependent Cre)

Depositor

Tatsushi Onaka

Article

Inutsuka et al Commun Biol. 2022 Sep 16;5(1):979. doi:

Insert

NCre-MBP5

Use

AAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLEX307-HCoV-NL63-3CL-C144A

Plasmid

#160287

Purpose

Allows for constituitive mammalian expression of the HCoV-NL63 3CL protease with C144A mutation via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Mutation

C144A

Promoter

EF1a

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pBabepuro3-p18 F37I

Plasmid

#24936

Depositor

Lynda Chin

Article

Wiedemeyer et al Cancer Cell. 2008 Apr . 13(4):355-64.

Insert

p18 (CDKN2C Human)

Use

Retroviral

Expression

Mammalian

Mutation

F37I

Available Since

June 23, 2010

Availability

Academic Institutions and Nonprofits only
pCI-neo-myc-LC3(deltaC22, G120A)

Plasmid

#45449

Depositor

Tamotsu Yoshimori

Article

Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

myc

Expression

Mammalian

Mutation

Encodes amino acids 1-120, Glycine at position 12…

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA HA-Gnat1 WT-3UTR

Plasmid

#184026

Purpose

Expresses mouse Gnat1 protein with N-terminal HA tag from cDNA that contains the full length 3'-UTR

Depositor

Peter Stoilov

Article

Matalkah et al Commun Biol. 2022 Sep 24;5(1):1011. doi:

Insert

Gnat1 (Gnat1 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pLEX307-MERS-CoV-3CL-C148A

Plasmid

#160283

Purpose

Allows for constituitive mammalian expression of the MERS-CoV 3CL protease with C145A mutation via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Mutation

C148A

Promoter

EF1a

Available Since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pSpCas9_BB_2A-GFP_MAPRE3-gRNA#2

Plasmid

#107730

Purpose

Knock out of EB3 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE3 gRNA #2 (targets Exon 1) (MAPRE3 Human)

Expression

Mammalian

Promoter

U6

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pSpCas9_BB_2A-GFP_MAPRE3-gRNA#1

Plasmid

#107729

Purpose

Knock out of EB3 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE3 gRNA #1 (targets Exon 1) (MAPRE3 Human)

Expression

Mammalian

Promoter

U6

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pET28-His6-Ubc9-C93A

Plasmid

#133910

Purpose

Bacterial expression of His6-Ubc9-C93A

Depositor

Jeffrey Bode

Article

Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

Insert

SUMO-conjugating enzyme UBC9 (UBE2I Human)

Tags

Thrombin-cleavable His6-tag

Expression

Bacterial

Mutation

Cysteine 93 to Alanine

Promoter

T7

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs-hGLIS1GR

Plasmid

#59312

Purpose

Forced expression of human GLIS1GR

Depositor

Shinya Yamanaka

Article

Takahashi et al Nat Commun. 2014 Apr 24;5:3678. doi: 10.

Insert

GLIS1 (GLIS1 Human)

Use

Retroviral

Tags

cGR - hormone binding domain of glucocorticoid re…

Expression

Mammalian

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-RNF8-Middle-part

Plasmid

#64672

Purpose

Expresses only Middle part of RNF8, lacking N and C terminus, amino acids 112-401

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

ring finger protein 8 (RNF8 Human)

Tags

GFP

Expression

Mammalian

Mutation

Containing only middle part of RNF8 amino acids 1…

Promoter

CMV

Available Since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-GABARα1N-HA

Plasmid

#128110

Purpose

Express GABARα1 N terminal domain

Depositor

Huda Zoghbi

Article

Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

Insert

Gabra1 (Gabra1 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX307-IBV-3CL-C143A

Plasmid

#160289

Purpose

Allows for constituitive mammalian expression of the IBV 3CL protease with C143A mutation via transfection or lentivirus

Depositor

Alejandro Chavez

Article

Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

Insert

3CL Protease

Use

Lentiviral

Expression

Mammalian

Mutation

C143A

Promoter

EF1a

Available Since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLR5-CAG-NLS-tdiRFP670-2A-LBR

Plasmid

#82620

Purpose

Expresses NLS-tdiRFP-2A-LBR in mammalian cells

Depositor

Hiroshi Ochiai

Article

Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

Insert

NLS-tdiRFP670-LBR (Lbr Synthetic, Mouse)

Use

Piggybac vector

Expression

Mammalian

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP Kind2 S181E

Plasmid

#105312

Purpose

To study altered downstream functions of phospholylation-site mimetic kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S181E

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP Kind2 S181A

Plasmid

#105307

Purpose

To study altered downstream functions of phospholylation-site null kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S181A

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223-GOLPH3

Plasmid

#21688

Depositor

Lynda Chin

Article

Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

Insert

Golgi Phosphoprotein 3 (GOLPH3 Human)

Use

Gateway entry clone

Mutation

last nucleotide of stop codon removed for c-termi…

Available Since

July 28, 2009

Availability

Academic Institutions and Nonprofits only
pAAV-EF1a-CCre-MBP1-WPRE

Plasmid

#193917

Purpose

Expresses one of the components of Cre-DOR_N6C1 (RFP-dependent Cre)

Depositor

Tatsushi Onaka

Article

Inutsuka et al Commun Biol. 2022 Sep 16;5(1):979. doi:

Insert

CCre-MBP1

Use

AAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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