166,556 results

1. pShuttle-CMV

   Plasmid

   #16403

   Purpose

   Shuttle vector for use in AdEasy System. Used for expression of transgenes under a CMV promoter when no GFP tracer is desired.

   Depositor

   Bert Vogelstein

   Article

   He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Adenoviral

   Expression

   Mammalian

   Available Since

   June 11, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. PCDNA3-b2AR-GFP11-T2A-GFP10-bArrestin1-T2A mCherry

   Plasmid

   #113610

   Purpose

   PPI reporter

   Depositor

   Xiaokun Shu

   Article

   Zhang et al Protein Sci. 2018 Apr;27(4):874-879. doi

   Insert

   beta 2 adrenergic receptor
   

   Expression

   Mammalian

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008)

   Plasmid

   #139997

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. BFP-Sec61 beta

   Plasmid

   #49154

   Purpose

   expression of Sec61 beta fused to BFP, used as a general ER marker

   Depositor

   Gia Voeltz

   Article

   Zurek et al Traffic. 2011 Jan;12(1):28-41. doi: 10.1

   Insert

   Sec61 beta (SEC61B Human)
   

   Tags

   BFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBFC0984

   Plasmid

   #231992

   Purpose

   CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with 2xBsaI spacer cloning site

   Depositor

   Brady Cress

   Article

   Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d

   Inserts

   dRfxCas13d
   crRNA with 2xBsaI spacer
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23119 and pTet

   Available Since

   April 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. L444P GBA pcDNA3.1

   Plasmid

   #188582

   Purpose

   Ectopic expression L444P GBA

   Depositor

   Anthony Schapira

   Article

   Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

   Insert

   GBA (GBA1 Human)
   

   Expression

   Mammalian

   Mutation

   L444P

   Promoter

   CMV

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-luciferase-P2A-Neo

   Plasmid

   #105621

   Purpose

   3rd generation lentiviral plasmid for expression of luciferase with Neo resistance

   Depositor

   Christopher Vakoc

   Article

   Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

   Insert

   Luciferase
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CaMKIIa(0.4)-PdCO-mScarlet-WPRE

   Plasmid

   #198508

   Purpose

   Expresses optimized PdCO in frame with mScarlet under control of minimal CamKIIa promotor.

   Depositor

   Ofer Yizhar

   Article

   Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

   Insert

   PdCO
   

   Use

   AAV

   Tags

   Rho1D4 and mScarlet

   Expression

   Mammalian

   Promoter

   CaMKIIα minimal promotor (0.4kb)

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pK170.AAV-TRE-Cre-WPRE (Supernova)

   Plasmid

   #85040

   Purpose

   For AAV-TRE-Cre based Supernova, pK170 should be used with pK168 etc.

   Depositor

   Takuji Iwasato

   Article

   Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

   Insert

   nlsCre-WPRE
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Nov. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. 7M8

    Plasmid

    #64839

    Purpose

    This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the 7M8 capsid protein.

    Depositor

    John Flannery, David Schaffer

    Article

    Dalkara et al Sci Transl Med. 2013 Jun 12;5(189):189ra

    Insert

    7M8 cap
    

    Use

    AAV

    Mutation

    7mer insertion in the AAV2 cap

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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