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pHiUGE V5-epitope donor ORF2

Plasmid

#200379

Purpose

Tagging endogenously expressed proteins with V5 epitope, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

V5-epitope

Expression

Mammalian

Mutation

NA

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pDGB3_omega2

Plasmid

#68239

Purpose

GoldenBraid level 1 Omega plant expression vector

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-Rap1b E63

Plasmid

#118328

Purpose

Expression of bovine Rap1b

Depositor

Philip Stork

Article

Takahashi et al J Biol Chem. 2013 Sep 27;288(39):27712-2

Insert

Rap1b E63 (RAP1B Bovine)

Tags

GFP

Expression

Mammalian

Mutation

Q63E

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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His6_BcLOV4_Q355N_mCherry_BamUK

Plasmid

#119762

Purpose

Encodes mCherry-tagged BcLOV4 permanently-"lit" mutant with Q355N mutation for expression in bacterial cell

Depositor

Brian Chow

Article

Glantz et al Methods Enzymol. 2019;622:249-270. doi:

Insert

BcLOV4-Q355N_mCherry

Tags

mCherry

Expression

Bacterial

Mutation

Mutated glutamine at position 355 to asparagine

Promoter

T7

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR221-SARS-CoV-2 S1 RBD

Plasmid

#167013

Purpose

Gateway-compatible Entry vector encoding spike (S1) RBD domain from SARS-CoV-2

Depositor

Spyros Petrakis

Article

Gkekas et al J Chem Inf Model. 2024 Nov 13. doi: 10.1

Insert

S (S SARS-CoV-2 isolate Wuhan-Hu-1)

Expression

Mammalian

Mutation

No stop codon

Available Since

April 5, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pLNHA-C1-HsGIGYF2_AE

Plasmid

#148550

Purpose

Mammalian Expression of HsGIGYF2

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF2 (GIGYF2 Human)

Expression

Mammalian

Mutation

one silent mutation and one deletion of Q1211 com…

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pZP06C

Plasmid

#232322

Purpose

pZP06C was constructed by adding the mCherry reporter gene driven by the rpsJ promoter into pZP06B (containing PrpsJ-sacB) to facilitate the screening of positive clones through red fluorescence.

Depositor

Chenggang Wu

Insert

mCherry

Expression

Bacterial

Promoter

rpsJ

Available Since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

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pDGB3_omega1

Plasmid

#68238

Purpose

GoldenBraid level 1 Omega plant expression vector

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha2

Plasmid

#68229

Purpose

GoldenBraid level 1 Alpha plant expression vector.

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 26, 2015

Availability

Academic Institutions and Nonprofits only

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R989-X27-566: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12C

Plasmid

#159547

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12C

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-188) G12C (KRAS Human)

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

G12C

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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