We narrowed to 15,723 results for: LEA

1. mEmerald-Actin-C-18

   Plasmid

   #53978

   Purpose

   Localization: Actin, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Article

   Burnette et al Nat Cell Biol. 2011 Apr;13(4):371-81. do

   Insert

   Actin (ACTB Human)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

2. MDH1-miR-221-PGK-GFP-Blast

   Plasmid

   #31519

   Depositor

   Chang-Zheng Chen

   Article

   Arnold et al Genome Res. 2011 May . 21(5):798-810.

   Insert

   miR-221 (Mir221 Mouse)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pAAV-EF1a-DIO-GRAB_g5-HT3.0mut

   Plasmid

   #208724

   Purpose

   Expresses the green 5-HT sensor GRAB_g5-HT3.0mut in neurons in the presence of Cre recombinase

   Depositor

   Yulong Li

   Article

   Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

   Insert

   Green fluorescent 5-HT sensor GRAB_g5-HT3.0mut
   

   Use

   AAV

   Promoter

   EF1a

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. FUW-TetO-Tfap2c

   Plasmid

   #128826

   Purpose

   dox-dependent expression of mouse Tfap2c

   Depositor

   Yossi Buganim

   Article

   Benchetrit et al Cell Stem Cell. 2015 Nov 5;17(5):543-56.

   Insert

   Tfap2c (Tfap2c Mouse)
   

   Use

   Lentiviral

   Available Since

   Aug. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Polymerase expression - pCMV-Phi29 (LM2708)

   Plasmid

   #208965

   Purpose

   Unfused Phi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   Phi29-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   Phi29(-exo;D169A)

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Nrf1-Myc pcDNA3.1(+)-C-Myc (NAWLVH106-AAAAAA)

   Plasmid

   #169126

   Purpose

   expression of protein

   Depositor

   Barbara Dirac-Svejstrup

   Article

   Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

   Insert

   hNrf1 (NFE2L1 Human)
   

   Expression

   Mammalian

   Mutation

   NAWLVH106-AAAAAA

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. shFGF2#2

   Plasmid

   #157668

   Purpose

   Tet inducible knockdown of FGF2

   Depositor

   Byung Il Lee

   Article

   Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

   Insert

   Fibroblast growth factor 2 (FGF2 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. mito-yHS1-M7A

   Plasmid

   #159164

   Purpose

   Mitochondrial labile heme reporter

   Depositor

   Amit Reddi

   Article

   Martinez-Guzman et al J Cell Sci. 2020 May 20;133(10). pii: jc

   Insert

   mito-yHS1-M7A
   

   Tags

   COX4 pre-sequence

   Expression

   Yeast

   Mutation

   Mutated Met 7 of the cyt b562 module of mito-yHS1…

   Promoter

   GPD

   Available Since

   Sept. 21, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pYPQ239

   Plasmid

   #108859

   Purpose

   FnCpf1 Gateway entry plasmid

   Depositor

   Yiping Qi

   Article

   Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

   Insert

   FnCpf1
   

   Use

   CRISPR; Gateway compatible cpf1 entry clone

   Tags

   NLS

   Expression

   Plant

   Mutation

   Cpf1 is rice codon optimized

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. nLuc_AP10_PPVs_P9mS

    Plasmid

    #119302

    Purpose

    Expresses N-terminus of split luciferase fused to antiparallel coiled-coil AP10 connected with autoinhibitory coil P9mS and PPVs cleavage site in antiparallel harpin linker/for SPOC logic

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split nLuc fused to antiparallel Coiled-coil AP10 connected with P9mS via PPV cleavable linker
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330-NQL005-SOX2-sgRNA

    Plasmid

    #175553

    Purpose

    For transient expression of spCas9-nuclease and a sgRNA targeting the mouse SOX2 locus.

    Depositor

    Elzo de Wit

    Article

    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

    Insert

    spCas9-nuclease and sgRNA against mouse SOX2 STOP Codon
    

    Use

    Mouse Targeting

    Expression

    Mammalian

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-YOD1-C160S

    Plasmid

    #85666

    Purpose

    Expression of human YOD1 C160S mutant with N-terminal GFP tag

    Depositor

    Hemmo Meyer

    Article

    Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

    Insert

    YOD1 (YOD1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    C160S

    Promoter

    CMV

    Available Since

    Feb. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn-NOS-IN133.3xFLAG.mCherry-WPRE

    Plasmid

    #127868

    Purpose

    pAAV plasmid expressing an NOS-IN133.3xFLAG.mCherry fusion protein under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

    Insert

    Nos1 (Nos1 Mouse)
    

    Use

    AAV

    Tags

    3xFLAG and mCherry

    Mutation

    Amino acids 1-133 only

    Promoter

    hSyn

    Available Since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. PUC57_BAG3P209L-eGFP

    Plasmid

    #98183

    Purpose

    Codes for full length, GFP tagged mutant Bag3

    Depositor

    Robert Bryson-Richardson

    Article

    Ruparelia et al Acta Neuropathol. 2014 Dec;128(6):821-33

    Insert

    BAG3P209L-eGFP (BAG3 Human)
    

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    P209L

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. LRRK2-K1906M

    Plasmid

    #35946

    Depositor

    Zhaoyang Feng

    Article

    Yuan et al PLoS One. 2011;6(8):e22354. Epub 2011 Au

    Insert

    LRRK2 (LRRK2 Human)
    

    Tags

    His and Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pFSynW Substance P-pHluorin IRES mRuby3

    Plasmid

    #195699

    Purpose

    Lentiviral plasmid encoding first 68 residues of mouse preprotachykinin with a C-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoter

    Depositor

    Edwin Chapman

    Article

    Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

    Insert

    Tac1 (Tac1 Mouse)
    

    Use

    Lentiviral

    Tags

    pHluorin

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBactin-Tubg1-mNeonGreen

    Plasmid

    #196869

    Purpose

    Expression of the gamma-tubulin fused to mNeonGreen

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Tubg1-mNeonGreen (Tubg1 Mouse)
    

    Expression

    Mammalian

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-EF1a-Itprip-V5

    Plasmid

    #120241

    Purpose

    Expresses V5-tagged Itprip in lentiviral vector

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Insert

    Itprip (ITPRIP Human)
    

    Use

    Lentiviral

    Tags

    V5

    Promoter

    EF1a

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSOMA-T679A-NLS

    Plasmid

    #118938

    Purpose

    Used to express the T679A mutant form of the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thaliana

    Depositor

    Patrick Krysan

    Article

    Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

    Insert

    SOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Localization Signal
    

    Expression

    Plant

    Available Since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR-TAF15N-miRFP670-Cry2WT

    Plasmid

    #122440

    Purpose

    Expresses disordered protein TAF15(1-208) fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

    Insert

    TAF15 (TAF15 Human)
    

    Use

    Lentiviral

    Tags

    Cry2WT and miRFP670

    Expression

    Mammalian

    Mutation

    Contains only amino acids 1-208

    Promoter

    SFFV

    Available Since

    March 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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