We narrowed to 15,600 results for: LEA

1. pDONR221-Ctdnep1_Cter

   Plasmid

   #196522

   Purpose

   Entry vector for Gateway with Ctdnep1_Cter

   Depositor

   Edgar Gomes

   Article

   Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

   Insert

   Ctdnep1-Cter (CTDNEP1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   No transmembrane domain

   Promoter

   CMV promoter; T7 promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR201-Ctdnep1

   Plasmid

   #196520

   Purpose

   Entry vector for Gateway with Ctdnep1

   Depositor

   Edgar Gomes

   Article

   Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

   Insert

   Ctdnep1-N1 (CTDNEP1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   CMV promoter; T7 promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMT-flk1-cytoBirA-2A-mCherry_Ras

   Plasmid

   #80056

   Purpose

   Flk1/Kdrl promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Tags

   3x HA

   Expression

   Bacterial

   Mutation

   Promoter

   flk1

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. HP1-ECFP-SNAP

   Plasmid

   #187966

   Purpose

   expresses HP1 with C-terminal tags ECFP and SNAP

   Depositor

   Markus Wahl

   Article

   Vester et al Commun Biol. 2022 Jul 22;5(1):736. doi:

   Insert

   HP1 (CBX5 Human)
   

   Use

   Tags

   ECFP-SNAP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTER E2F3shRNA human

   Plasmid

   #66885

   Purpose

   Doxycycline-regulated mammalian expression vector for expressing shRNA against E2F3

   Depositor

   Maria Alvarado-Kristensson

   Article

   Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

   Insert

   E2F transcription factor 3 (E2F3 Human)
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1 Tet

   Available since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pCAG-F-API5

   Plasmid

   #157662

   Purpose

   Expresses API5 in mammalian cell

   Depositor

   Byung Il Lee

   Article

   Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

   Insert

   Apoptosis inhibitor 5 (API5 Human)
   

   Use

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMKO.1-shBirc5

   Plasmid

   #160949

   Purpose

   Birc5 shRNA in pMKO.1 retroviral vector

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Birc5 shRNA (Birc5 Mouse)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFPN1-N-gamma-tubulin (1-333)

   Plasmid

   #87858

   Purpose

   Fluorescent fragment of gamma-tubulin that lacks gamma-tubulin DNA binding domain and NLS

   Depositor

   Maria Alvarado-Kristensson

   Article

   Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

   Insert

   gamma-tubulin 1 (TUBG1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Fragment from aa 1 to 333

   Promoter

   Available since

   May 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. MO70 - Orai1 EC-Flag

   Plasmid

   #79590

   Purpose

   Transient expression and retroviral Expression

   Depositor

   Yousang Gwack

   Article

   Srikanth et al J Biol Chem. 2010 Feb 12;285(7):5066-75.

   Insert

   Orai1 (ORAI1 Human)
   

   Use

   Retroviral

   Tags

   FLAG (inserted between TM3 and TM4 of the protein…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pCAG-lox-inv[Talpha1-iCre-pA]-lox-Lyn-EGFP

    Plasmid

    #196875

    Purpose

    Neuron-specific expression of LynGFP reporter

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    LynEGFP
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSOMA-T679A-NES

    Plasmid

    #118940

    Purpose

    Used to express the T679A mutant form of the SOMA FRET sensor with a nuclear exclusion signal in Arabidopsis thaliana

    Depositor

    Patrick Krysan

    Article

    Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

    Insert

    SOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Exclusion Signal
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pClneoMyc MOB1 T74A

    Plasmid

    #47939

    Purpose

    expresses Myc tagged human MOB1 containing T74A mutation

    Depositor

    Yutaka Hata

    Article

    Bao et al Genes Cells. 2009 Dec;14(12):1369-81. do

    Insert

    MOB1A (MOB1A Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    T74A

    Promoter

    CMV

    Available since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pGBT9-Hook2

    Plasmid

    #198524

    Purpose

    Expression of GAL4 DNA-binding domain (BD)-Hook2 fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

    Insert

    Hook2 (HOOK2 Human)
    

    Use

    Tags

    GAL4-DNA binding domain fragment

    Expression

    Yeast

    Mutation

    His488Gln substitution

    Promoter

    ADH1

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28b-API5

    Plasmid

    #157655

    Purpose

    Expresses API5 in E.coli cell

    Depositor

    Byung Il Lee

    Article

    Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

    Insert

    Apoptosis inhibitor 5 (API5 Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. sTR056

    Plasmid

    #177369

    Purpose

    Toehold switch, with an unpaired toehold region at the 5′-end that interacts with the trigger RNA (sTR060, Addgene plasmid # 177370) to unfold the switch.

    Depositor

    Jerome Bonnet, Jean-Loup Faulon

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Insert

    sfGFP
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. sTR060

    Plasmid

    #177370

    Purpose

    Trigger RNA (GCAGGGATAAACGAGATAGATAAGATAAGA) that pairs with the toehold region in sTR056 (Addgene plasmid #177369) to restore translational activity.

    Depositor

    Jerome Bonnet, Jean-Loup Faulon

    Article

    Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

    Insert

    Trigger RNA
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCS2+ oDam_f_GFPoNLS

    Plasmid

    #85819

    Purpose

    iDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

    Depositor

    Joachim Wittbrodt

    Article

    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

    Insert

    oDam_f_GFPoNLS
    

    Use

    Tags

    oNLS (optimized nuclear localization signal) C te…

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    L122A. Codon optimization compatible to work in M…

    Promoter

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. p3xFLAG-MKL1 D301-380

    Plasmid

    #19852

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1 (MRTFA Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    deleted amino acids 301 to 380

    Promoter

    Available since

    Dec. 16, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pEGFP-YOD1-MutS2

    Plasmid

    #85667

    Purpose

    Expression of human YOD1 I292Q V295Q mutant with N-terminal GFP tag

    Depositor

    Hemmo Meyer

    Article

    Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

    Insert

    YOD1 (YOD1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    I292Q and V295Q

    Promoter

    CMV

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-EF1a-V5-Emerin

    Plasmid

    #120246

    Purpose

    Expresses V5-tagged Emerin in lentiviral vector

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Insert

    Emerin (Emd Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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