We narrowed to 11,247 results for: SHA

1. pFA6a-kanMX6-P41nmt1-GFP

   Plasmid

   #39290

   Depositor

   John Pringle, Jurg Bahler

   Article

   Bahler et al Yeast. 1998 Jul;14(10):943-51.

   Insert

   nmt1 promoter (nmt1 Fission Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Expression

   Mutation

   -1163 to +6 of nmt1 locus with a 4bp deletion (TA…

   Promoter

   Available since

   Aug. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pXPR_BRD003 sgKEAP1-2

   Plasmid

   #74986

   Purpose

   sgKEAP1-2, puromycin selection

   Depositor

   William Hahn

   Article

   Krall et al Elife. 2017 Feb 1;6:e18970. doi: 10.7554

   Insert

   KEAP1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(-)-Gαi3-YFP (Citrine variant, b/c loop insert)

   Plasmid

   #171375

   Purpose

   For the mammalian expression of rat Gαi3 internally tagged with YFP

   Depositor

   Mikel Garcia-Marcos

   Article

   Marivin et al Sci Signal. 2016 Apr 12;9(423):ra37. doi

   Insert

   Gai3-YFP
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGZ110 (pFA6a-3FLAG-MNase-TRP1)

   Plasmid

   #70233

   Purpose

   ChEC-tagging vector encoding a 3xFLAG tag and aa 83-231 of Mnase in pFA6a-3HA-Trp1 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope tag

   Depositor

   Steven Henikoff

   Article

   Zentner et al Nat Commun. 2015 Oct 22;6:8733. doi: 10.

   Insert

   3xFLAG tag and aa 83-231 of Mnase
   

   Use

   Yeast genomic targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDB.His.MBP.3C NLRP1 CARD WT

   Plasmid

   #164008

   Purpose

   Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 CARD

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   His6-MBP-3C

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSCV FLIPi P2G_Thy1.1 p53

   Plasmid

   #19748

   Depositor

   Richard Hynes

   Article

   Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

   Insert

   oligo targeting p53 (Trp53 Mouse)
   

   Use

   Cre/Lox, RNAi, and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pC1300_pUB10_pco-vCASphi_E9t_V2_CmYLCVp_35sT_ribozyme_AtPDS3_gRNA10

   Plasmid

   #197966

   Purpose

   T-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by CmYLCVp, flanked by ribozymes.

   Depositor

   Steven Jacobsen

   Article

   Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

   Inserts

   pco-vCasphi-2
   AtPDS3 gRNA10
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CmYLCVp and pUBQ10

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMM9-MAPKK2-KW71 [delta(48-55)/S222D/S226D]

   Plasmid

   #40806

   Depositor

   Natalie Ahn

   Article

   Whalen et al Mol Cell Biol. 1997 Apr;17(4):1947-58.

   Insert

   MAP2K2 (MAP2K2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta(48-55) + S222D + S226D

   Promoter

   MSV-LTR

   Available since

   Nov. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

9. FLAG-MED8

   Plasmid

   #15406

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Tomomori-Sato et al J Biol Chem. 2004 Feb 13. 279(7):5846-51

   Insert

   MED8 (MED8 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pC010 LshCas13a locus with nontargeting spacer

    Plasmid

    #91901

    Purpose

    LshCas13a locus into pACYC184 with nontargeting spacer

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

    Insert

    LshCas13a locus with non-targeting spacer
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 16, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pUAST-SIK3 T196E

    Plasmid

    #52975

    Purpose

    insect expression of SIK3 T196E constitutively active mutant

    Depositor

    Biao Wang, Marc Montminy

    Article

    Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

    Insert

    SIK3 (Sik3 Fly)
    

    Use

    Tags

    HA

    Expression

    Insect

    Mutation

    T196E constitutively active mutant

    Promoter

    Hsp70

    Available since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pX330-PITCh-H3C2

    Plasmid

    #207780

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H3C2 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of H3C2 (H3C2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. 1M-Tm-antimCherry

    Plasmid

    #206952

    Purpose

    Mammalian expression of 1M-Tm EMcapsulins targeting mCherry

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    MmMT3, TmEnc, antimCherry intrabody (LaM-4)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. 1M-Qt-antiGFP

    Plasmid

    #206951

    Purpose

    Mammalian expression of 1M-Qt EMcapsulins targeting GFP

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    MmMT3, QtEnc, antiGFP-intrabody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAFMW-Nbr-D435A/E437A

    Plasmid

    #40829

    Purpose

    Expresses Flag, Myc tagged mutant Nbr in Drosophila cells

    Depositor

    Phillip Zamore

    Article

    Han et al Curr Biol. 2011 Nov 22;21(22):1878-87. E

    Insert

    Nbr D435A/E437A (Nbr Fly)
    

    Use

    Tags

    3xFLAG and 6xMyc

    Expression

    Insect

    Mutation

    D435A/E437A, part of the DEDD motif; mutations p…

    Promoter

    Actin5C

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pCAG-mCherry-FKBP-GEF(TIAM1)

    Plasmid

    #85156

    Purpose

    acute activation of Rac through rapamycin-induced translocation to membrane-localized FRB

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Insert

    Fusion of mCherry, FKBP, and the DH-domain of human TIAM1 (amino acids 1012-1250)
    

    Use

    Tags

    mCherry-FKBP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. p23-NES-PguCas13b-msfGFP-NES-Flag

    Plasmid

    #165072

    Purpose

    overexpression of PguCas13b in human cells

    Depositor

    Ling-Ling Chen

    Article

    Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

    Insert

    PguCas13b
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJL1-mRFP1 gRNA

    Plasmid

    #117052

    Purpose

    In vitro expression of anti-mRFP1 Cas9 sgRNA from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

    Insert

    anti-mRFP1 SpCas9 gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. YFP-Rpp25-C1

    Plasmid

    #134544

    Purpose

    Expresses YFP-Rpp25 fusion protein

    Depositor

    Susan Janicki

    Article

    Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

    Insert

    Rpp25 (RPP25 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLXSN-FLK1 TM

    Plasmid

    #65249

    Purpose

    Expression of dominant negative FLK1 in mammalian cells

    Depositor

    Axel Ullrich

    Article

    Millauer et al Nature. 1994 Feb 10;367(6463):576-9.

    Insert

    FLK1 TM (Kdr Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    deleted AA 786-1346

    Promoter

    LTR

    Available since

    Dec. 22, 2016

    Availability

    Academic Institutions and Nonprofits only