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GFP-MUTED

Plasmid

#164629

Purpose

Expresses MUTED in mammalian cells with a GFP tag

Depositor

Mark Cookson

Article

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

MUTED (BLOC1S5 Human)

Tags

cycle 3 GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 12, 2021

Availability

Academic Institutions and Nonprofits only

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pEF-Bos TRAM Flag

Plasmid

#41551

Depositor

Kate Fitzgerald, Doug Golenbock

Article

Fitzgerald et al J Exp Med. 2003 Oct 6;198(7):1043-55. Ep

Insert

TRAM (TICAM2 Human)

Tags

6xHis and Flag

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 14, 2012

Availability

Academic Institutions and Nonprofits only
pCRI006-pYFAC-riboB-PgpdA-dSpCas9-VPR-TtrpC

Plasmid

#140199

Purpose

Episomal expression of dSpCas9-VPR. Filamentous fungi vector with AMA1 and riboB selection marker.

Depositor

Yit Heng Chooi

Article

Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

Insert

dSpCas9-VPR

Use

CRISPR and Synthetic Biology; Expression in asper…

Tags

VPR (VP64-p65-Rta), NLS

Promoter

PgpdA

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCAX-mScarlet-FBP17

Plasmid

#178366

Purpose

Expresses FBP17 c-terminal labeled with mScarelt in mammalian cells

Depositor

Erik Dent

Article

Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

Insert

Formin Binding Protein 17 (FNBP1 Human)

Expression

Mammalian

Mutation

400 Glutamic Acid changed to Aspartic acid (403 A…

Promoter

Chicken Beta Actin

Available Since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pCRI004-pYFAC-riboB-PgpdA-dLbCas12a-VPR-TtrpC

Plasmid

#140197

Purpose

Episomal expression of dLbCas12a-VPR. Filamentous fungi vector with AMA1 and riboB selection marker.

Depositor

Yit Heng Chooi

Article

Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

Insert

dLbCas12a-VPR

Use

CRISPR and Synthetic Biology; Expression in asper…

Tags

VPR (VP64-p65-Rta), NLS

Mutation

D832A DNase deactivated

Promoter

PgpdA

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pSCGB3A2_HL-P2A-TagBFP-PGK-NeoR-SCGB3A2_HR

Plasmid

#126697

Purpose

donor vector for targeting a 2A peptide followed by blue fluorescent reporter to the human SCGB3A2 locus at the end of the endogenous coding sequence

Depositor

Darrell Kotton

Article

McCauley et al Stem Cell Reports. 2018 May 8;10(5):1579

Insert

TagBFP

Expression

Mammalian

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-SCGB3A2_gRNA1-SpCas9-T2A-GFP

Plasmid

#126698

Purpose

to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human SCGB3A2 locus at the end of the endogenous coding sequence

Depositor

Darrell Kotton

Article

McCauley et al Stem Cell Reports. 2018 May 8;10(5):1579

Insert

sgRNA targeting human SCGB3A2 locus

Expression

Mammalian

Promoter

Human U6

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/HIS/FLAG/HA-EDF1

Plasmid

#38079

Depositor

Markus Landthaler

Article

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

EDF1 endothelial differentiation-related factor 1 (EDF1 Human)

Tags

HIS/FLAG/HA

Expression

Mammalian

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pTriEX-Antenna-GDI Rac1

Plasmid

#83359

Purpose

Expression of Antenna-Rac1

Depositor

Klaus Hahn

Article

Hodgson et al Nat Chem Biol. 2016 Aug 8. doi: 10.1038/

Insert

Rac1 (RAC1 Human)

Tags

FRET Antenna (mCerulean-cpVenus) and His

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

March 6, 2017

Availability

Academic Institutions and Nonprofits only

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2xmyc-RAB35

Plasmid

#164632

Purpose

Expresses RAB35 in mammalian cells with a 2xmyc tag

Depositor

Mark Cookson

Article

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

RAB35 (RAB35 Human)

Tags

2xmyc

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCSGFPnERT(#205)

Plasmid

#184065

Purpose

cyclofen-inducible GFP nuclear relocation via mammalian cell transfection or mRNA synthesis

Depositor

Sophie Vriz

Article

Sinha et al Chembiochem. 2010 Mar 22;11(5):653-63. d

Insert

eGFP-nls-ERT2

Use

Synthetic Biology

Expression

Mammalian

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

sCMV+SP6

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+ Flag-RAGB99L

Plasmid

#128101

Purpose

Expresses constitutively GTP-bound RAGB in mammalian cells in a G418-selectable plasmid

Depositor

Chi Van Dang

Article

Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

Insert

Ras related GTP binding B (RRAGB Human)

Tags

Flag

Expression

Mammalian

Mutation

Q99L (CAA to CTA)

Promoter

CMV

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pBabe-PI(WT)-Rac1(WT)-mCherry

Plasmid

#87550

Purpose

PI(WT): Photo-inhibitable/blue light sensitive Rac1

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
Rac1 (RAC1 Human)

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pMXs-puro HA-Atg14*dCC

Plasmid

#38266

Depositor

Noboru Mizushima

Article

Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

Insert

autophagy related genes 14 (ATG14 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

changed A618T, A621T, C624A and T627C for siRNA r…

Promoter

LTR

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
pBabe-PI(WT)-Rac1(Q61L)-mCherry

Plasmid

#87553

Purpose

PI(WT): Photo-inhibitable/blue light sensitive; const. active Rac1

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
Rac1 (RAC1 Human)

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

Q61L

Promoter

CMV

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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ArpC3-FLAG_pLib

Plasmid

#173680

Purpose

ArpC3 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

Depositor

Roberto Dominguez

Article

Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

Insert

ArpC3 (ARPC3 Human)

Tags

TEV cleavage site and FLAG tag

Expression

Insect

Promoter

polyhedrin

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-TRR-C421-Y2404F

Plasmid

#182846

Purpose

mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 2011-2431 aa). Expression in E. coli. by IPTG induction

Depositor

Peter Harte

Article

Tie et al Development. 2014 Mar;141(5):1129-39. do

Insert

TRR 2011-2431 (trr Fly)

Expression

Bacterial

Mutation

mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 20…

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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RCAS-sgATG7

Plasmid

#75228

Purpose

An adaption on the RCAS/tv-a somatic cell gene transfer system, for use in combination with an existing Cas9 background in the cell/mouse of interest. Depositors: Jane Fraser/Noor Gammoh

Depositor

Noor Gammoh

Article

Fraser et al EMBO Rep. 2019 Oct 4;20(10):e47734. doi:

Insert

Autophagy Related 7 (Atg7 Mouse)

Use

Retroviral

Promoter

U6

Available Since

June 2, 2016

Availability

Academic Institutions and Nonprofits only
pMIR-Report-SPRED1-3'UTR (WT - miR-206 site)

Plasmid

#62577

Purpose

Translational Luciferase Reporter containing a fragment of the 3'UTR of SPRED1 containing a miR-206 binding site

Depositor

Michael Ruppert

Article

Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

Insert

SPRED1 (SPRED1 Human)

Use

Luciferase

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pEMS1210

Plasmid

#29107

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple238 (TRH Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Nov. 4, 2011

Availability

Academic Institutions and Nonprofits only

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