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pICSL4723-P1

Plasmid

#191786

Purpose

Level P Golden gate accepter

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Type

Empty backbone

Expression

Plant

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pAC-Luc

Plasmid

#33154

Depositor

Michael Rosbash

Article

Kadener et al Genes Dev. 2007 Jul 1;21(13):1675-86. Ep

Type

Empty backbone

Use

Luciferase

Tags

Luciferase

Expression

Insect

Promoter

Actin 5C

Available Since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
pHygRR2

Plasmid

#200075

Purpose

hygR selection mScarlet 2X marked rapid RMCE integration vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

Type

Empty backbone

Use

Recombination mediated cassette exchange

Expression

Worm

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pJMP2846

Plasmid

#160676

Purpose

Mobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAs

Depositor

Jason Peters

Article

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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moxVenus

Plasmid

#68068

Purpose

C-terminal fusion to moxVenus

Depositor

Erik Snapp

Article

Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

Type

Empty backbone

Tags

moxVenus

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pJH2971

Plasmid

#100955

Purpose

Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. KANMX marker

Depositor

James Haber

Article

Ranjith et al Protocol Exchange (2017)

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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MG

Plasmid

#26528

Depositor

David Baltimore

Article

O'Connell et al J Exp Med. 2008 Mar 17. 205(3):585-94.

Type

Empty backbone

Use

Retroviral

Tags

eGFP

Expression

Mammalian

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

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Sticky-355-3X

Plasmid

#159644

Purpose

Non-coding DNA sequence for dinucleosome preparation

Depositor

Curt Davey

Article

Adhireksan et al Nat Commun. 2020 Sep 21;11(1):4747. doi:

Type

Empty backbone

Use

Bacterial vector for dna production

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAK501

Plasmid

#48107

Purpose

lacZ promoter-probe plasmid with pVS1 origin of replication and chloramphenicol resistance

Depositor

Julia Vorholt

Article

Kaczmarczyk et al Appl Environ Microbiol. 2013 Nov;79(21):

Type

Empty backbone

Expression

Bacterial

Available Since

Dec. 8, 2013

Availability

Academic Institutions and Nonprofits only

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pYZ155

Plasmid

#98411

Purpose

Pombe Expression Vector - nmt1 promoter with Sp.ura4 marker

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pG20_mCherry_Hyg

Plasmid

#159701

Purpose

pGREEN-based cloning construct for expression in planta

Depositor

Hans-Henning Kunz

Article

Pratt et al MicroPubl Biol. 2020 Oct 7;2020. doi: 10

Type

Empty backbone

Tags

mCherry

Expression

Plant

Available Since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pBABE-EGFP

Plasmid

#36999

Depositor

Debu Chakravarti

Article

Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

May 21, 2012

Availability

Academic Institutions and Nonprofits only
pQCH

Plasmid

#164160

Purpose

IncQ BHR plasmid

Depositor

Calvin Henard

Article

Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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psi-CHECK3

Plasmid

#136010

Purpose

psi-CHECK2 plasmid modified so both Renilla and Firefly luciferase contain introns

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Type

Empty backbone

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pQFT

Plasmid

#203674

Purpose

pQF derivative with promoter PQ5 exchanged for promoter PQJ

Depositor

Urs Jenal

Article

Klotz et al Appl Environ Microbiol. 2023 May 18:e002

Type

Empty backbone

Tags

3xFLAG

Expression

Bacterial

Promoter

PQJ

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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p354_DG

Plasmid

#234697

Purpose

Golden gate domesticated pET28b for scarless cloning with His10-TEV

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

His10-TEV

Expression

Bacterial

Mutation

No

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pSC27

Plasmid

#117478

Purpose

mobilizable, SRB-stable, kanamycin-resistant cloning vector

Depositor

Judy Wall

Article

Rousset et al Plasmid. 1998;39(2):114-22. doi: 10.1006

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP×3-N1

Plasmid

#86776

Purpose

A mammalian expression vector with triple EGFP at C-terminus.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2017

Availability

Academic Institutions and Nonprofits only

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pIYC04

Plasmid

#64741

Purpose

Yeast expression plasmid

Depositor

Jens Nielsen

Article

Chen et al Metab Eng. 2013 Jan;15:48-54. doi: 10.10

Type

Empty backbone

Expression

Yeast

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pPD95.75-wVenus

Plasmid

#37464

Depositor

Hitoshi Sawa

Article

Yamamoto et al PLoS Genet. 2011 Oct;7(10):e1002308. Epu

Type

Empty backbone

Expression

Worm

Promoter

none

Available Since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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mCerulean-pBAD

Plasmid

#54666

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Type

Empty backbone

Tags

6xHis and mCerulean

Expression

Bacterial

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only
pV1393:SpRY

Plasmid

#172189

Purpose

Genome editing of C. albicans

Depositor

Doug Bernstein

Article

Evans et al mSphere. 2021 May 19;6(3). pii: 6/3/e003

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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HyGCaMP6s

Plasmid

#102558

Purpose

Expression of GCaMP6s in Hydra

Depositor

Rafael Yuste

Article

Dupre et al Curr Biol. 2017 Apr 24;27(8):1085-1097.

Insert

GCaMP6s

Use

Hydra expression

Tags

Actin/GCaMP6s fusion protein (C terminal on backb…

Available Since

Oct. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pSEVAT7K1A

Plasmid

#58302

Purpose

GeneGuard vector cassette: thyA, ColE2 COR, Kid toxin; with ampR as cargo.

Depositor

Tom Ellis

Article

Wright et al ACS Synth Biol. 2014 May 13.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pIB170

Plasmid

#90193

Purpose

E. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pveg promoter

Depositor

Indranil Biswas

Article

Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

Type

Empty backbone

Tags

None

Expression

Bacterial

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pIB165

Plasmid

#90188

Purpose

E. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoter

Depositor

Indranil Biswas

Article

Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

Type

Empty backbone

Tags

His-tag and Strep-tag

Expression

Bacterial

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pNIC-HIS10-ZBASIC-TEV-GG

Plasmid

#215797

Purpose

Cloning of target gene with N-terminal His10 Zbasic tag and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available Since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pRoRL

Plasmid

#213132

Purpose

Riboptoregulator circuit RoRL for blue-light-activated gene expression

Depositor

Andreas Moeglich

Article

Ranzani et al Nucleic Acids Res. 2024 Aug 10:gkae678.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pZC06 (pFA6a-PSc-6xGly-PrtA-KanMX)

Plasmid

#44090

Depositor

Tim Formosa

Type

Empty backbone

Use

Pcr-based yeast c-terminal tagging

Tags

6xGly linker, ADH1 transcription terminator, KanM…

Available Since

April 18, 2013

Availability

Academic Institutions and Nonprofits only

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pJMP2844

Plasmid

#160675

Purpose

Mobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAs

Depositor

Jason Peters

Article

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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