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pCDNA5/FRT-ChemoG1

Plasmid

#193806

Purpose

CMV overexpression of ChemoG1 in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG1

Tags

10xHis

Expression

Mammalian

Mutation

EGFP: A206K

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pFS_0351_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_1

Plasmid

#116963

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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py100-sgRNA_hVEGFA

Plasmid

#198282

Purpose

Expression of SpCas9 guide RNA specific for human VEGFA locus

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

py100-sgRNA_hVEGFA

Expression

Mammalian

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pSTAP-seq_human_spike-in

Plasmid

#125152

Purpose

Empty vector to clone and measure the expression of promoter candidates (spike-in) by determining the abundance of transcripts originating from the candidate in human cells

Depositor

Alexander Stark

Article

Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

Type

Empty backbone

Use

Stap-seq screening vector

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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TwinStrep-SUMO-Pnf

Plasmid

#198321

Purpose

For affinity purification of Pnf payload

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

TwinStrep-SUMO-Pnf

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-MoonTag

Plasmid

#198299

Purpose

Produces PVCs retargeted with MoonTag

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-MoonTag

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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nCas9-NG_P2A-mCherry

Plasmid

#219934

Purpose

Mammalian expression of Sp-nCas9-NG with P2A-mCherry marker for use with MOBE systems

Depositor

Alexis Komor

Article

Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

Insert

Sp-nCas9(D10A)-NG_P2A-mCherry

Use

CRISPR

Tags

SV40 bpNLS and SV40 bpNLS-P2A-mCherry

Expression

Mammalian

Mutation

nCas9(D10A)

Promoter

CMV

Available Since

June 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_COX8-CoA-Snifit(G41S)

Plasmid

#197946

Purpose

Expression of CoA-Snifit in mammalian cells

Depositor

Kai Johnsson

Article

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Insert

Halo-GFP-ecPanK

Expression

Mammalian

Mutation

PanK=PanK-G41S, D127A,L277W,F252Y

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pTW194b63

Plasmid

#136930

Purpose

Directed evolution of SpCas10

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

npuC-gIII(C)

Use

CRISPR

Expression

Bacterial

Mutation

none

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pBT138b-pro1

Plasmid

#138513

Purpose

P3h

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

T7-RNAP

Use

Synthetic Biology

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pDESTmycTNRC6C

Plasmid

#19874

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

Trinucleotide repeat containing 6C (TNRC6C Human)

Tags

MYC

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pET-51b(+) ChemoG1

Plasmid

#193799

Purpose

Protein production of ChemoG1 in bacteria

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG1

Tags

10xHis

Expression

Bacterial

Promoter

T7

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pET-51b(+) ChemoG2

Plasmid

#193800

Purpose

Protein production of ChemoG2 in bacteria

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG2

Tags

10xHis

Expression

Bacterial

Mutation

EGFP: A206K

Promoter

T7

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pET-51b(+) ChemoG3

Plasmid

#193801

Purpose

Protein production of ChemoG3 in bacteria

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG3

Tags

10xHis

Expression

Bacterial

Mutation

EGFP:A206K; HT7: L271E

Promoter

T7

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pET-51b(+) ChemoG4

Plasmid

#193804

Purpose

Protein production of ChemoG4 in bacteria

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG4

Tags

10xHis

Expression

Bacterial

Mutation

EGFP:A206K; HT7: E143R, E147R, L271E

Promoter

T7

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pFS_0353_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_2

Plasmid

#116965

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCR1075

Plasmid

#111093

Purpose

Expresses eGFP donor in pUC19

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

eGFP

Expression

Mammalian

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pFS_0143-pET30-Fusicatenibacter_saccharivorans

Plasmid

#116952

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1, compatible with classic acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pBM19-sd4U

Plasmid

#179692

Purpose

Bacterial expression of base editor

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

[sd4U] UGI-TALE4-DddA-G1397-C (T1413I)

Expression

Bacterial

Mutation

T1413I

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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3XFlag-pLPC

Plasmid

#73560

Purpose

vector encoding 3 Flag tags

Depositor

Gerardo Ferbeyre

Article

Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

Insert

3X-Flag

Use

Retroviral

Tags

3X-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only