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FUW-tetO-hOCT4

Plasmid

#20726

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Cell Stem Cell. 2008 Sep 11. 3(3):346-53

Insert

OCT4 (POU5F1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 13, 2009

Availability

Academic Institutions and Nonprofits only

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Fz4-GFP

Plasmid

#42197

Depositor

Robert Lefkowitz, Jeremy Nathans

Article

Chen et al Science. 2003 Sep 5;301(5638):1391-4.

Insert

Frizzled 4 (Fzd4 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc3-bTrCP

Plasmid

#20718

Depositor

Yue Xiong

Article

Ohta et al Cancer Res. 2001 Feb 15. 61(4):1347-53.

Insert

beta-transducin repeat containing (BTRC) (Btrc Mouse)

Tags

Myc3

Expression

Mammalian

Mutation

*

Available Since

May 1, 2009

Availability

Academic Institutions and Nonprofits only

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UBC plasmid

Plasmid

#169746

Purpose

Unregulated "open-loop" UBC promoter plasmid. This plasmid encodes eGFP to report circuit output levels.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

EGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

UBC

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pKOR1

Plasmid

#133446

Purpose

Escherichia coli/S. aureus shuttle vector, permits rapid cloning via lambda recombination and ccdB selection

Depositor

Taeok Bae, Olaf Schneewind

Article

Bae et al Plasmid. 2006 Jan;55(1):58-63. doi: 10.1

Type

Empty backbone

Use

E.coli/s.aureus shuttle vector

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pSL-MS2-6X

Plasmid

#27118

Depositor

Robert Singer

Article

Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

Insert

MS2 binding sites (6)

Expression

Bacterial

Available Since

Dec. 20, 2010

Availability

Academic Institutions and Nonprofits only

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pTNT-B18R-6His

Plasmid

#58979

Purpose

expresses His-tagged B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNs

Depositor

Steven Dowdy

Article

Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

Insert

B18R

Tags

His

Expression

Bacterial

Promoter

T7/Sp6

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti-DsRed_IRES_EGFP

Plasmid

#92194

Purpose

Lentiviral overexpression vector to make stable bicistronic cell line for control (EGFP) screen

Depositor

Huda Zoghbi

Article

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

DsRed-IRES-EGFP

Use

Lentiviral

Expression

Mammalian

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

Plasmid

#60231

Purpose

Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

Depositor

Feng Zhang

Article

Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

Inserts

sgRNA
Cre recombinase
EGFP

Use

AAV, CRISPR, Cre/Lox, and Mouse Targeting

Tags

Cre-HA-P2A and EGFP-KASH

Expression

Mammalian

Promoter

U6 and hSyn

Available Since

Oct. 9, 2014

Availability

Academic Institutions and Nonprofits only

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Flag Nprl2 pRK5

Plasmid

#46333

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Nprl2 (NPRL2 Human)

Tags

Flag

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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mEmerald-GPAT4152-208

Plasmid

#134468

Purpose

GPAT4152-208 expression

Depositor

Jennifer Lippincott-Schwartz

Article

Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

Insert

GPAT4 (GPAT4 Human)

Expression

Mammalian

Mutation

GPAT4, amino acids 152-208

Promoter

CMV

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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EFSp-GFP-Empty

Plasmid

#174171

Purpose

Control lentiviral vector expressing GFP under control of the EFS promoter.

Depositor

Rod Bremner

Article

Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

EFS

Available Since

Aug. 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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6xHis-MBP-BrCas12b-RFND

Plasmid

#195339

Purpose

Expresses Engineered BrCas12b in Bacteria

Depositor

Piyush Jain

Article

Nguyen et al Cell Rep Med. 2023 May 16;4(5):101037. d

Insert

BrCas12b

Use

CRISPR and Synthetic Biology

Tags

6xHis and 6xHis, MBP, TEV cleavage site

Expression

Bacterial

Mutation

R160E, F208W, N524V, D868V

Promoter

T7

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-rGBD

Plasmid

#26732

Depositor

William Bement

Article

Benink et al J Cell Biol. 2005 Jan 31. 168(3):429-39.

Insert

rhotekin (rtkn2 Mouse)

Use

Reporter for rho

Tags

GFP

Expression

Mammalian

Mutation

RhoA binding domain of rhotekin

Available Since

Feb. 25, 2011

Availability

Academic Institutions and Nonprofits only

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SypHer

Plasmid

#48250

Purpose

pH sensor

Depositor

Nicolas Demaurex

Article

Poburko et al J Biol Chem. 2011 Apr 1;286(13):11672-84

Insert

SypHer

Expression

Mammalian

Mutation

C199S

Promoter

CMV

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only
p3xFLAG-TYR

Plasmid

#32780

Depositor

Ruth Halaban

Article

Halaban et al J Invest Dermatol. 2002 Aug;119(2):481-8

Insert

Tyrosinase (TYR Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2011

Availability

Academic Institutions and Nonprofits only
gRNA Cloning Vector Bbs I ver. 2

Plasmid

#85586

Purpose

An empty sgRNA expression vector. sgRNA sequence can be cloned into its Bbs I site. Its gRNA scaffold has efficient ver. 2 structure.

Depositor

Hodaka Fujii

Article

Fujita et al Sci Rep. 2016 Jul 28;6:30485. doi: 10.10

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pmScarlet-i_Giantin_C1

Plasmid

#85050

Purpose

In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Giantin (GOLGB1 Human)

Expression

Mammalian

Mutation

aa 3131-3259

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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CMV-R-GECO1

Plasmid

#32444

Purpose

Mammalian expression of Red fluorescent genetically encoded Ca2+ indicator for optical imaging

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

R-GECO1.0

Expression

Mammalian

Mutation

Substitutions relative to the mApple-derived anal…

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pLPCX roGFP2-Orp1

Plasmid

#64991

Purpose

Mammalian expression of cytosolic roGFP2-Orp1 (retroviral vector)

Depositor

Tobias Dick

Article

Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

Insert

Orp1 (HYR1 Budding Yeast)

Use

Retroviral

Tags

roGFP2

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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