19,658 results

1. pHAGE-EGFR-L858R

   Plasmid

   #116276

   Purpose

   Lentiviral expression of EGFR L858R

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   EGFR (EGFR Human)
   

   Use

   Lentiviral

   Mutation

   L858R

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-beta-HA-BRCA2

   Plasmid

   #133553

   Purpose

   Mammalian expression vector with CMV promoter driving beta globin intron, HA tag, BRCA2 ORF

   Depositor

   David Livingston

   Article

   Chen et al Mol Cell. 1998 Sep;2(3):317-28.

   Insert

   BRCA2 (BRCA2 Human)
   

   Expression

   Mammalian

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. KIF1A(1-393)-GCN4-2xmCherry-EF(C)

   Plasmid

   #61665

   Purpose

   Tandem mCherry-labeled, forced-dimeric, truncated kinesin-3 fused to EF Hand linker

   Depositor

   Kristen Verhey

   Article

   Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

   Insert

   KIF1A(1-393)-GCN4-2xmCherry-EF(C) (KIF1A Human)
   

   Expression

   Mammalian

   Available Since

   March 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA

   Plasmid

   #86613

   Purpose

   Vector for tandem expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

   Insert

   ATP1A1 G3 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
   

   Use

   CRISPR; Co-selection via hdr using ouabain

   Expression

   Mammalian

   Mutation

   K848A, K1003A, & R1060A

   Promoter

   CBh

   Available Since

   March 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTRC-APEX2

   Plasmid

   #72558

   Purpose

   Expression of cytosolic APEX2 in bacteria

   Depositor

   Alice Ting

   Article

   Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

   Insert

   APEX2
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   A134P relative to APEX

   Available Since

   May 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLentiCas9-BFP

   Plasmid

   #78545

   Purpose

   Fusion protein Cas9+BFP

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   BFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Fusion protein with Cas9

   Available Since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP

   Plasmid

   #165830

   Purpose

   EGFP green fluorescent protein CDS

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   EGFP
   

   Expression

   Insect

   Available Since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT7-V5-SBP-C1-HshnRNPK

   Plasmid

   #64923

   Purpose

   Expresses V5- and SBP-tagged hnRNPK in mammalian cells

   Depositor

   Elisa Izaurralde

   Insert

   hnRNPK (HNRNPK Human)
   

   Tags

   V5 and SBP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUCDM

   Plasmid

   #110742

   Purpose

   Plasmid for the insect cell expression system, donor for plasmid fusions, two promoters, Multiplication Module M, LoxP site

   Depositor

   Tim Richmond

   Article

   Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

   Type

   Empty backbone

   Expression

   Insect

   Promoter

   Polyhedrin and p10 promoters

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-FLAG-KLF5

    Plasmid

    #40918

    Depositor

    Jin-Tang Dong

    Article

    Zhao et al Biochem J. 2011 Jul 15;437(2):323-33.

    Insert

    Kruppel-like factor 5 (KLF5 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-U6gRNA1-U6gRNA2-TnT-Cre

    Plasmid

    #87682

    Purpose

    AAV vector for U6 driven expression of two gRNAs, and cardiomyocyte specific expression of Cre recombinase.

    Depositor

    William Pu

    Article

    Guo et al Circ Res. 2017 Mar 29. pii: CIRCRESAHA.1

    Inserts

    gRNA1
    gRNA2
    Cre
    

    Use

    AAV

    Promoter

    U6 and cTnT

    Available Since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE-CTCF

    Plasmid

    #116728

    Purpose

    Lentiviral expression of CTCF

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    CTCF (CTCF Human)
    

    Use

    Lentiviral

    Mutation

    WT

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Synapsin-pm-iATPSnFR1.0

    Plasmid

    #102555

    Purpose

    expresses membrane displayed ATP sensor in neurons

    Depositor

    Baljit Khakh

    Article

    Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

    Insert

    iATPSnFR1.0
    

    Use

    AAV

    Available Since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEB2-mKate2

    Plasmid

    #104009

    Purpose

    Plasmid encoding mKate2

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mKate2
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Mutations relative to eqFP578 (R32G, K42R, V45A, …

    Promoter

    proC

    Available Since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDEST/N1-hBIN1-GFP

    Plasmid

    #27305

    Depositor

    Robin Shaw

    Article

    Hong et al PLoS Biol. 2010 Feb . 8(2):e1000312.

    Insert

    bridging integrator 1 (BIN1), transcript variant 8 (BIN1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pINDUCER21-RORA

    Plasmid

    #51303

    Purpose

    Expression of RORA after doxycycline administration

    Depositor

    George Daley

    Article

    Doulatov et al Cell Stem Cell. 2013 Oct 3;13(4):459-70.

    Insert

    RAR-related orphan receptor A (RORA Human)
    

    Use

    Lentiviral

    Tags

    n/a

    Mutation

    full length ORF

    Available Since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCherry-VP40

    Plasmid

    #74421

    Purpose

    Mammalian expression of Ebolavirus matrix protein VP40 with an mCherry fusion

    Depositor

    Judith White

    Article

    Shoemaker et al PLoS One. 2013;8(2):e56265. doi: 10.1371

    Insert

    VP40
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pgRNAtet-lvaA

    Plasmid

    #106397

    Purpose

    Guide RNA plasmid targeting lvaA on BBR1-UP origin

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    sgRNA towards lvaA in Pseudomonas putida
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR1A no ccDB (w48-1)

    Plasmid

    #17398

    Purpose

    Gateway entry 1A vector, no ccDB gene

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Entry vector

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV-FLIM-AKAR

    Plasmid

    #63058

    Purpose

    Expresses the Fluorescence Lifetime Imaging Microscopy (FLIM)-compatible PKA activity sensor FLIM-AKAR in an AAV backbone

    Depositor

    Bernardo Sabatini

    Article

    Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

    Insert

    FLIM-AKAR
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    EF-1α

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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