We narrowed to 154,128 results for: ins;
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Plasmid#74981PurposeConstitutive ETV1 ORF, blasticidin selectionDepositorAvailable SinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only
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pRDB_053
Plasmid#216083Purposefor transgenic flies; gypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31, destination vectorDepositorHas ServiceCloning Grade DNAInsertgypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31
UseDestinationExpressionInsectAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG-LoxP-GFP-LoxP-SNAP-TERT
Plasmid#71391PurposeHomologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes GFP marker flanked by LoxP sites for selection.DepositorAvailable SinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
CD-MPR-mCherry-RUSH
Plasmid#202798PurposeRUSH cargo: Str-KDEL_IRES_SBP-mCherry-CD-MPRDepositorInsertM6PR (M6PR Human)
TagsmCherryExpressionMammalianMutationChanged 40 K (AAA) with R (AGA)PromoterCMVAvailable SinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro-CAG-fl-STOP-fl-ZEB2-GFP-Flag-WPRE-poly(A)
Plasmid#165456PurposeInducible ZEB2 expression from AAVS1 locusDepositorAvailable SinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX:PGRMC1
Plasmid#136062PurposeBacterial expression of pGEX:PGRMC1 to perform a GST-pulldown assay and identify new proteins that interact with GST-PGRMC1 in the human endometrial stroll cells.DepositorInsertProgesterone receptor membrane component 1 (PGRMC1 Human)
TagsGST-PGRMC1ExpressionBacterialAvailable SinceMay 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIDK-HDAC1fl
Plasmid#109158PurposeEncodes human full-length HDAC1 to be expressed in a baculovirus/insect cell expression systemDepositorInserthuman full-length HDAC1, sequence-optimized for insect cells (HDAC1 Human)
ExpressionInsectPromoterp10Available SinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRRL.SIN.EF1A.JAG1-B5.GS.CAR-3G
Plasmid#194464PurposeCAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.B5 in VH-VL order & (GGGGS)3 linker (scFv). GFP-Zeo for selection & monitoring.DepositorInsertAnti-JAG1 CAR
UseLentiviralAvailable SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
Gene Trap - 24XPP7 Lentiviral Vector
Plasmid#174199PurposeLentiviral expression vector coding for Blasticidin, iRFP and 24X PP7 hairpins. All inserts are cloned in on the -strand to allow for splicing in when targeted into an intron of transcribed genes.DepositorInsertiRFP
UseLentiviral; VectorTags24X PP7 hairpins, Blasticidin, and T2AExpressionMammalianAvailable SinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTarget
Plasmid#214052PurposeExpresses target sequence for Haliangium type III CRISPR-Cas complex; identical to pNon-target (Addgene plasmid # 214053) but contains a protospacer.DepositorInsertTarget sequence
UseTarget rna plasmidExpressionBacterialPromotertrc promoterAvailable SinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHochTypeIII
Plasmid#214039PurposeBacterial expression plasmid for Haliangium ochraceum type III CRISPR-Cas complex; contains csb2, a minimal CRISPR array with a pTarget spacer, cmr1-6DepositorInsertCas10
UseCRISPRMutationWTPromoterT7 promoterAvailable SinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hHBEGF
Plasmid#199234PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertProheparin-binding EGF-like growth factor (HBEGF Human)
TagsTrxA-6xHis-S-tagExpressionBacterialPromoterT7Available SinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mVenus-PA-Rac1
Plasmid#22007PurposeExpression of photoactivatable Rac1DepositorInsertPA-Rac1 (RAC1 Human, Avena sativa (oat))
Tags6X His and mVenusExpressionBacterial, Insect, and Mamm…MutationLOV(Leu404-Leu546), Rac1 starts at I4 and contain…PromoterCMV, p10Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pB80-KIF1A(1-365)-VVDfast-mVenus-SSPB(micro)_P2A_iLID-mCherry-RAB11
Plasmid#174644PurposeOptogenetic coupling of RAB11 to opto-kinesin to induce anterograde transport of recycling endosomesDepositorInsertKIF1A(1-365)-VVDfast-mVenus-SSPB(micro)-P2A-iLID-mCherry-RAB11
TagsVenus-SSPB and iLID-mCherryExpressionMammalianMutationmmKIF1A(aa1-365): Pro202Ala; mVenus: Met1Del, Thr…PromoterChicken beta-actinAvailable SinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX Emt-SH2
Plasmid#46424DepositorInsertEmt (ITK Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 223-346PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT_KCNQ3
Plasmid#173163PurposeTo generate Flp-In CHO-KCNQ3 stable cellsDepositorAvailable SinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX Src-SH2
Plasmid#46510DepositorInsertSrc (SRC Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 142-252PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+) NRP1 b1 (273-427)
Plasmid#162734PurposeBacterial expression of the b1 domain from the human Neuropilin 1 receptor (NRP1). With an N-terminal 6His tag and thrombin cleavage site.DepositorInsertHuman NRP1 b1 domain residues 273-427
ExpressionBacterialMutationCodon optimisedAvailable SinceApril 27, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
EBA175-bio
Plasmid#47743PurposeExpresses enzymatically monobiotinylated full-length EBA175 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.DepositorInsertCodon-optimised EBA175
Tagsenzymatic biotinylation sequence and rat Cd4 d3+4ExpressionMammalianMutationExogenous signal peptide of mouse origin, changed…PromoterCMVAvailable SinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
eGFP-PRM-5R
Plasmid#112155PurposeMammalian expression plasmid for PRM-5R in a vector expressing eGFP.DepositorInsertPRM-5R (ABL1 Human)
ExpressionMammalianMutationcontains only the PRM domain of Abl1PromoterCMVAvailable SinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only